Objective: The expression of the epithelial cell adhesion molecule (EpCAM) is correlated with a poor prognosis, and treatment failure early tumor recurrence of hepatocellular carcinoma (HCC) patients. The tumor microenvironment determine the fate of tumor cancer stem cell-start (CSC); However, very limited studies that seek to evaluate CSC tumorigenesis in the liver microenvironment. Here, we have systematically investigated the role of EpCAM + cancer cells in tumor initiation in an orthotopic model of HCC.
Result: control mice and mice with bland steatosis failed to develop tumors. In rats with steatohepatitis, EpCAM + CSC has demonstrated a significant improvement in terms of the ability of tumor initiation and growth, as compared to non-CSC EpCAM- inoculation (p <0.005). For Hep3B inoculation, high-EpCAM group has demonstrated tumor growth was significantly higher compared with EpCAM-Low (p <0.005). For HepG2 inoculation, both high EpCAM and EpCAM-Low group confirmed the same tumor incidence and growth.
Methods: Diet-induced compromised microenvironments fatty liver was established to mimic the clinical and patient non-alcoholic steatohepatitis (NASH) and the ability of tumorigenic cells Hepa1-6 evaluated. CSC is enriched by the cultural round and labeled with copGFP for EpCAM + CSC and with mCherry for non-CSC. FACS-sorted cells were inoculated into the left lobe of the liver, and the tumor growth was monitored by high frequency ultrasound. The Hep3B and HepG2 cell subpopulations in terms of EpCAM-low and high EpCAM was evaluated in an orthotopic model of athymic mice.
Conclusion: NASH micro EpCAM + CSC began promoting tumorigenesis in a mouse model of immunocompetent. EpCAM showed differential expression of different tumor biology in athymic mouse model.
Multimodal imaging of stem cell-derived bulge of hair follicles in a mouse model of traumatic brain injury.
traumatic brain injury (TBI) is a devastating event therapy is currently limited. Stem cell transplantation may lead to recovery of function through different mechanisms, such as the replacement of cells through differentiation, angiogenesis stimulation and support for micro environment. Adult hair follicle bulge-derived stem cells (HFBSCs) has a capacity of neuronal differentiation, it is easy to harvest and relatively immune-privileged, which makes them potential candidates for autologous stem cell-based therapies.
In this study, we apply the in vivo multimodal techniques of optical and magnetic resonance imaging to investigate the behavior of HFBSCs mouse in a mouse model of TBI. HFBSCs stated Luc2 and copGFP and examined for their differentiation capacity in vitro. Furthermore, HFBSCs transduced, loaded with ferumoxytol, transplanted next TBI lesions (cortical region) in nude mice, two days after the injury. Brains were fixed for immunohistochemistry 58 days after transplantation. Luc2- and copGFP-express, HFBSCs ferumoxytol-loaded show sufficient differentiation potential of neurons in the brain lesions revealed vitro.
Bioluminescence HFBSCs survival and magnetic resonance imaging identified their localization in the field of transplantation. Immunohistochemistry showed that the transplanted cells stained for nestin and Neurofilament protein (NF-Pan). Cells also expressed laminin and fibronectin extracellular matrix but the mass was not detected.
 pCDH-CMV-MCS-t2A-copGFP cDNA Cloning and Expression Vector |
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| CD524A-1 | SBI | 10 ug | EUR 653 |
|
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 pCDH-MCS-T2A-Puro-MSCV cDNA Cloning and Expression Vector |
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| CD522A-1 | SBI | 10 ug | EUR 653 |
|
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 pCDH- EF1- MCS- T2A- copGFP |
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| PVT2336 | Lifescience Market | 2 ug | EUR 325 |
 pCDH-CMV-MCS-EF1-copGFP cDNA Cloning and Expression Vector |
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| CD511B-1 | SBI | 10 ug | EUR 635 |
|
|||
 pCDH-MSCV-MCS-EF1-Puro cDNA Cloning and Expression Vector |
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| CD710B-1 | SBI | 10 ug | EUR 635 |
|
|||
 pCDH-MSCV-MCS-EF1-GFP cDNA Cloning and Expression Vector |
|||
| CD711B-1 | SBI | 10 ug | EUR 635 |
|
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 pCDH-MSCV-MCS-IRES-GFP cDNA Cloning and Expression Vector |
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| CD731B-1 | SBI | 10 ug | EUR 653 |
|
|||
 pCDH-MSCV-MCS-EF1-GFP+Puro cDNA Cloning and Expression Vector |
|||
| CD713B-1 | SBI | 10 ug | EUR 635 |
|
|||
 pCDH- CMV- MCS- EF1- CopGFP- T2A- Puro |
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| PVT2333 | Lifescience Market | 2 ug | EUR 325 |
 Cloning and Expression Vector) pCDH-EF1-MCS-(PGK-GFP-T2A-Puro) Cloning and Expression Vector |
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| CD813A-1 | SBI | 10 ug | EUR 642 |
|
|||
 Cloning and Expression Vector) pCDH-EF1-MCS-T2A-RFP (PGK-Puro) Cloning and Expression Vector |
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| CD822A-1 | SBI | 10 ug | EUR 642 |
|
|||
 Cloning and Expression Vector) pCDH-EF1-MCS-T2A-GFP (PGK-Puro) Cloning and Expression Vector |
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| CD823A-1 | SBI | 10 ug | EUR 642 |
|
|||
 pCDH-CMV-MCS-EF1-copGFP-T2A-Puro plasmid |
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| PVT17271 | Lifescience Market | 2 ug | EUR 474 |
 pCDH-EF1-MCS-T2A-Puro cDNA Cloning and Expression Vector 10 ug |
|||
| CD527A-1 | SBI | 10 ug | EUR 653 |
|
|||
 pCDH-CMV-MCS cDNA Cloning and Expression Vector |
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| CD500B-1 | SBI | 10 ug | EUR 544 |
|
|||
 pCDH-EF1-MCS cDNA Cloning and Expression Vector |
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| CD502A-1 | SBI | 10 ug | EUR 544 |
|
|||
 pCDH-EF1-MCS-BGH-PGK-GFP-T2A-Puro cDNA Cloning and Expression Vector |
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| CD550A-1 | SBI | 10 ug | EUR 625 |
|
|||
 Cloning and Expression Vector) pCDH-EF1-MCS-(PGK-Puro) Cloning and Expression Vector |
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| CD810A-1 | SBI | 10 ug | EUR 642 |
|
|||
 Cloning and Expression Vector) pCDH-EF1-MCS-(PGK-GFP) Cloning and Expression Vector |
|||
| CD811A-1 | SBI | 10 ug | EUR 642 |
|
|||
 pCDH-EF1-Luc-T2A-copGFP |
|||
| PVT17649 | Lifescience Market | 2 ug | EUR 424 |
 PB-MSCV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector |
|||
| PB713B-1 | SBI | 10 ug | EUR 702 |
|
|||
 pCDH-CMV-MCS-EF1-Puro cDNA Cloning and Expression Vector |
|||
| CD510B-1 | SBI | 10 ug | EUR 635 |
|
|||
 pCDH-CMV-MCS-EF1-RFP cDNA Cloning and Expression Vector |
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| CD512B-1 | SBI | 10 ug | EUR 635 |
|
|||
 pCDH-CMV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector |
|||
| CD513B-1 | SBI | 10 ug | EUR 635 |
|
|||
 pCDH-CMV-MCS-EF1-Neo cDNA Cloning and Expression Vector |
|||
| CD514B-1 | SBI | 10 ug | EUR 635 |
|
|||
 pCDH-CMV-MCS-EF1-Hygro cDNA Cloning and Expression Vector |
|||
| CD515B-1 | SBI | 10 ug | EUR 635 |
|
|||
 pCDH-EF1-MCS-IRES-GFP cDNA Cloning and Expression Vector |
|||
| CD530A-2 | SBI | 10 ug | EUR 653 |
|
|||
 pCDH-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector |
|||
| CD531A-2 | SBI | 10 ug | EUR 653 |
|
|||
 pCDH-EF1-MCS-IRES-Puro cDNA Cloning and Expression Vector |
|||
| CD532A-2 | SBI | 10 ug | EUR 653 |
|
|||
 pCDH-EF1-MCS-IRES-Neo cDNA Cloning and Expression Vector |
|||
| CD533A-2 | SBI | 10 ug | EUR 653 |
|
|||
 pCDH-UbC-MCS-EF1-Hygro cDNA Cloning and Expression Vector |
|||
| CD615B-1 | SBI | 10 ug | EUR 635 |
|
|||
 pCDH-UbC-MCS-IRES-GFP cDNA Cloning and Expression Vector |
|||
| CD630A-1 | SBI | 10 ug | EUR 653 |
|
|||
 pCDH-EF1-MCS*-T2A-GFP |
|||
| CD526A-1 | SBI | 10 ug | EUR 653 |
|
|||
 pCDH- CMV- MCS- EF1- copGFP Plasmid |
|||
| PVT2334 | Lifescience Market | 2 ug | EUR 325 |
 pAAVK-EF1? -MCS-T2A-EGFP AAVanced Cloning and Expression Vector |
|||
| AAV526A-1 | SBI | 10 ug | EUR 677 |
|
|||
 pAAVK-EF1? -MCS-T2A-Puro AAVanced Cloning and Expression Vector |
|||
| AAV527A-1 | SBI | 10 ug | EUR 677 |
|
|||
 pAAVK-EF1? ?MCS-T2A-mRFP AAVanced Cloning and Expression Vector |
|||
| AAV528A-1 | SBI | 10 ug | EUR 677 |
|
|||
 pCDH-CMV-MCS-EF1-RFP+Puro cDNA Cloning and Expression Vector |
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| CD516B-2 | SBI | 10 ug | EUR 635 |
|
|||
 XStamp cloning and expression lentivector MSCV-Leader-MCS-C1C2-EF1-Puro |
|||
| XSTP710PA-1 | SBI | 10 ug | EUR 797 |
|
|||
 pCDH- EF1- MCS- T2A- Puro Plasmid |
|||
| PVT2335 | Lifescience Market | 2 ug | EUR 325 |
 pCDH-CMV-Akaluc-EF1-CopGFP-T2A-Puro |
|||
| PVT14490 | Lifescience Market | 2 ug | EUR 599 |
 pCDH-CuO-MCS-T2A-GFP co-inducible T2A lentivector |
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| QM521A-1 | SBI | 10 ug | EUR 679 |
|
|||
 pCDH-CuO-MCS-T2A-RFP co-inducible T2A lentivector |
|||
| QM522A-1 | SBI | 10 ug | EUR 679 |
|
|||
 pCDH-Cuo-MCS-EF1-CymR-T2A-Puro |
|||
| QM800A-1 | SBI | 10 ug | EUR 1310 |
|
|||
 pCDH- CMV- MCS- EF1- turboRFP- T2A- Puro |
|||
| PVT11077 | Lifescience Market | 2 ug | EUR 370 |
 plasmid) pCDH-EF1-MCS-T2A-Puro (CD520A) plasmid |
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| PVT17273 | Lifescience Market | 2 ug | EUR 474 |
 pSIH1-H1-copGFP shRNA Cloning and Expression Vector |
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| SI501A-1 | SBI | 10 ug | EUR 567 |
|
|||
 pCDH-CMV-TXNL1-HA-EF1-CopGFP-T2A-Puro |
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| PVT17693 | Lifescience Market | 2 ug | EUR 258 |
 pCDH-CMV-CCDC68-HA-EF1-CopGFP-T2A-Puro |
|||
| PVT17760 | Lifescience Market | 2 ug | EUR 258 |
 pCDH-CMV-ccl3-m-EF1-CopGFP-T2A-Puro |
|||
| PVT19054 | Lifescience Market | 2 ug | EUR 300 |
 pCDH-CMV-PTX3-EF1-CopGFP-T2A-Puro Plasmid |
|||
| PVT17116 | Lifescience Market | 2 ug | EUR 325 |
 pCDH-CMV-efemp1-m-EF1-CopGFP-T2A-Puro |
|||
| PVT17438 | Lifescience Market | 2 ug | EUR 300 |
 pCDH-CMV-MCS2 cDNA Cloning and Expression Vector |
|||
| CD501A-1 | SBI | 10 ug | EUR 544 |
|
|||
 pAAVK-EF1? -MCS AAVanced Cloning and Expression Vector |
|||
| AAV502A-1 | SBI | 10 ug | EUR 677 |
|
|||
 pCDF1-MCS2-EF1-copGFP cDNA Cloning and Expression Vector |
|||
| CD111B-1 | SBI | 10 ug | EUR 635 |
|
|||
 pCDH-CuO-MCS empty cloning lentivector |
|||
| QM500A-1 | SBI | 10 ug | EUR 597 |
|
|||
 BLIV 2.0 Reporter: MSCV-Luciferase-EF1a-copGFP-T2A-Puro Lentivector Plasmid |
|||
| BLIV713PA-1 | SBI | 10 ug | EUR 803 |
|
|||
 pCDH-CMV-MCS-EF1-copGFP cDNA Clon/Exp Vector [pre-packaged virus] |
|||
| CD511VB-1 | SBI | >1 x 10^6 IFUs | EUR 786 |
|
|||
 pCDH-CMV-MCS-EF1-copGFP+Puro cDNA Clon/Exp Vector [pre-packaged] |
|||
| CD513VB-1 | SBI | >1 x 10^6 IFUs | EUR 786 |
|
|||
) Scramble control hairpin in pCDH-CMV-MCS-EF1-copGFP (CD511B-1) |
|||
| PMIRH000PA-1 | SBI | Bacterial Streak | EUR 656 |
|
|||
 PB-CMV-GreenPuro-H1-MCS shRNA cloning and expression vector |
|||
| PBSI505A-1 | SBI | 10 ug | EUR 702 |
|
|||
 PB-EF1-GreenPuro-H1-MCS shRNA cloning and expression vector |
|||
| PBSI506A-1 | SBI | 10 ug | EUR 702 |
|
|||
 PB-CMV-MCS-EF1-Puro cDNA cloning and expression vector |
|||
| PB510B-1 | SBI | 10 ug | EUR 702 |
|
|||
 PB-CMV-MCS-EF1-GFP cDNA cloning and expression vector |
|||
| PB511B-1 | SBI | 10 ug | EUR 702 |
|
|||
 PB-CMV-MCS-EF1-RFP cDNA cloning and expression vector |
|||
| PB512B-1 | SBI | 10 ug | EUR 702 |
|
|||
 PB-CMV-MCS-EF1-GreenPuro cDNA cloning and expression vector |
|||
| PB513B-1 | SBI | 10 ug | EUR 702 |
|
|||
 PB-CMV-MCS-EF1-RedPuro cDNA Cloning and Expression Vector |
|||
| PB514B-2 | SBI | 10 ug | EUR 702 |
|
|||
 PB-EF1-MCS-IRES-GFP cDNA cloning and expression vector |
|||
| PB530A-2 | SBI | 10 ug | EUR 702 |
|
|||
 PB-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector |
|||
| PB531A-2 | SBI | 10 ug | EUR 702 |
|
|||
 PB-EF1-MCS-IRES-Neo cDNA cloning and expression vector |
|||
| PB533A-2 | SBI | 10 ug | EUR 702 |
|
|||
 XPack MSCV-XP-MCS-EF1-Puro Cloning Lentivector |
|||
| XPAK710PA-1 | SBI | 10 ug | EUR 797 |
|
|||
 BLIV 2.0 Reporter: MSCV-Luciferase-EF1a-copGFP-T2A-Puro Pre-packaged Virus |
|||
| BLIV713VA-1 | SBI | >2 x10^6 IFUs | EUR 803 |
|
|||
 pAAV-MCS Expression Vector |
|||
| VPK-410 | Cell Biolabs | 10 µg | EUR 647 |
|
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV. |
|||
 pscAAV-MCS Expression Vector |
|||
| VPK-430 | Cell Biolabs | 10 µg | EUR 647 |
|
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV. |
|||
 pCDH- CMV- mCherry- EF1- CopGFP- Puro |
|||
| PVT11078 | Lifescience Market | 2 ug | EUR 370 |
 pCDH-CMV-GSTA1-EF1-copGFP Plasmid |
|||
| PVT16093 | Lifescience Market | 2 ug | EUR 325 |
 T2A-GFP Co-Expression HR Targeting Vector [T2A-GFP-pA-loxP-EF1?-RFP-T2A-Puro-pA-LoxP-MCS] |
|||
| HR130PA-1 | SBI | 10 ug | EUR 1023 |
|
|||
 pCDH- CMV- MCS- EF1- puro |
|||
| PVT11075 | Lifescience Market | 2 ug | EUR 370 |
 pCDH- CMV- MCS- EF1- Hygro |
|||
| PVT11076 | Lifescience Market | 2 ug | EUR 370 |
 pCDH-EF1-Luc2-T2A-ZsGreen1 |
|||
| PVT17917 | Lifescience Market | 2 ug | EUR 424 |
 pCDH-CMV-MCS-EF1-copGFP cDNA Clon/Exp Vector [pre-packaged Ultra-High Titer virus] |
|||
| CD511VB-UHT | SBI | >1x10^9 IFUs | EUR 1041 |
|
|||
 pCDH-CMV-exosome booster-EF1-copGFP-Puro vector |
|||
| PVT12109 | Lifescience Market | 2 ug | EUR 958 |
 pAAV-MCS Promoterless Expression Vector |
|||
| VPK-411 | Cell Biolabs | 10 µg | EUR 647 |
|
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV. |
|||
) pMC.CMV-GFP-T2A-Puro-H1-MCS (shRNA expression parental minicircle plasmid) |
|||
| MNSI505A-1 | SBI | 10 ug | EUR 847 |
|
|||
) pMC.EF1-GFP-T2A-Puro-H1-MCS (shRNA expression parental minicircle plasmid) |
|||
| MNSI506A-1 | SBI | 10 ug | EUR 847 |
|
|||
 GFP-T2A-Luciferase Co-Expression HR Targeting Vector [GFP-T2A-Luc-pA-loxP-EF1?-RFP-T2A-Puro-pA-LoxP-MCS] |
|||
| HR150PA-1 | SBI | 10 ug | EUR 1023 |
|
|||
 pCDH- ECMV- MCS- 3*Flag- EF1 |
|||
| PVT11080 | Lifescience Market | 2 ug | EUR 370 |
 pCDH-CMV-MCS-EF1a-GFP+BSD |
|||
| PVT17282 | Lifescience Market | 2 ug | EUR 391 |
 pCDH-CMV-MCS-EF1-EGFP-IRFP |
|||
| PVT17586 | Lifescience Market | 2 ug | EUR 424 |
 pCDH- EF1- luc2- T2A- Tdtomato Plasmid |
|||
| PVT2337 | Lifescience Market | 2 ug | EUR 241 |
) pCDH-EF1-CymR-T2A-Puro (plasmid) |
|||
| QM200PA-2 | SBI | 10 ug | EUR 597 |
|
|||
) pCDH-EF1-CymR-T2A-Puro (virus) |
|||
| QM200VA-2 | SBI | >1 x 10^6 IFUs | EUR 636 |
|
|||
) pCDH-EF1-CymR-T2A-RFP (plasmid) |
|||
| QM300PA-1 | SBI | 10 ug | EUR 597 |
|
|||
) pCDH-EF1-CymR-T2A-RFP (virus) |
|||
| QM300VA-1 | SBI | >1 x 10^6 IFUs | EUR 636 |
|
|||
) pCDH-EF1-CymR-T2A-Neo (plasmid) |
|||
| QM400PA-2 | SBI | 10 ug | EUR 581 |
|
|||
) pCDH-EF1-CymR-T2A-Neo (virus) |
|||
| QM400VA-2 | SBI | >1 x 10^6 IFUs | EUR 620 |
|
|||
 pCDH-NFAT-Luc2-T2A-tdTomato Plasmid |
|||
| PVT16080 | Lifescience Market | 2 ug | EUR 325 |
 pGreen- Puro- pSIH1- H1- copGFP- T2A- Puro |
|||
| PVT11116 | Lifescience Market | 2 ug | EUR 370 |
 PB-Cuo-MCS-IRES-GFP-EF1-CymR-Puro Inducible cDNA Cloning and Expression Vector |
|||
| PBQM812A-1 | SBI | 10 ug | EUR 1096 |
|
|||
 MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid |
|||
| CASLV120PA-1 | SBI | 10 ug | EUR 697 |
|
|||
 pCDH-CuO-MCS-IRES-GFP-EF1-CymR-T2A-Puro All-in-one inducible IRES lentivector |
|||
| QM812B-1 | SBI | 10 ug | EUR 1310 |
|
|||
 plasmid) pCDH-CMV-MCS-EF1-Puro (CD510B) plasmid |
|||
| PVT17272 | Lifescience Market | 2 ug | EUR 474 |
 pCDF1-MCS1 cDNA Cloning and Expression Vector |
|||
| CD100A-1 | SBI | 10 ug | EUR 544 |
|
|||
 pCDH-CMV-GLUT1-EF1-turboRFP-T2A-Puro |
|||
| PVT13588 | Lifescience Market | 2 ug | EUR 599 |
 pCDH-ME-APOE-Luc2-T2A-tdTomato Plasmid |
|||
| PVT17253 | Lifescience Market | 2 ug | EUR 370 |
 pCDH-CuO-MCS-EF1-GFP+Puro dual promoter and dual marker lentivector |
|||
| QM513B-1 | SBI | 10 ug | EUR 679 |
|
|||
 pCDH-CuO-MCS-EF1-RFP+Puro dual promoter and dual marker lentivector |
|||
| QM516B-2 | SBI | 10 ug | EUR 679 |
|
|||
 pCDH-CuO-MCS-EF1-GFP dual promoter lentivector |
|||
| QM511B-1 | SBI | 10 ug | EUR 679 |
|
|||
 pCDH-CuO-MCS-EF1-RFP dual promoter lentivector |
|||
| QM512B-1 | SBI | 10 ug | EUR 679 |
|
|||
 pCDH-CMV-MCS-EF1-Puro-FLAG-HIF2 alpha |
|||
| PVT12166 | Lifescience Market | 2 ug | EUR 370 |
 EEV cloning and expression vector) Constitutive (CAGs promoter) EEV cloning and expression vector |
|||
| EEV600A-1 | SBI | 10 ug | EUR 677 |
|
|||
 EEV cloning and expression vector) Cumate-Inducible (CuO) EEV cloning and expression vector |
|||
| EEV610A-1 | SBI | 10 ug | EUR 677 |
|
|||
 pSIH1-H1-Puro shRNA Cloning and Expression Vector |
|||
| SI500A-1 | SBI | 10 ug | EUR 567 |
|
|||
 pSIH1-H1-H2Kk shRNA Cloning and Expression Vector |
|||
| SI502A-1 | SBI | 10 ug | EUR 567 |
|
|||
) pCDH-Cuo-RFP-T2A-GFP (positive control plasmid) |
|||
| QM350PA-1 | SBI | 10 ug | EUR 597 |
|
|||
) pCDH-Cuo-RFP-T2A-GFP (positive control virus) |
|||
| QM350VA-1 | SBI | >1 x 10^6 IFUs | EUR 636 |
|
|||
 pCDH-CuO-MCS-IRES-GFP co-inducible IRES lentivector |
|||
| QM530A-2 | SBI | 10 ug | EUR 679 |
|
|||
 pCDH-CuO-MCS-IRES-RFP co-inducible IRES lentivector |
|||
| QM531A-2 | SBI | 10 ug | EUR 679 |
|
|||
 XPack MSCV-XP-GFP-EF1-Puro Expression Lentivector |
|||
| XPAK730PA-1 | SBI | 10 ug | EUR 826 |
|
|||
 XPack MSCV-XP-GFP-EF1-Puro Expression Lentivirus |
|||
| XPAK730VA-1 | SBI | >1 x 10^6 IFUs | EUR 826 |
|
|||
 XPack MSCV-XP-Luciferase-EF1-Puro Expression Lentivector |
|||
| XPAK732PA-1 | SBI | 10 ug | EUR 826 |
|
|||
 XPack MSCV-XP-Luciferase-EF1-Puro Expression Lentivirus |
|||
| XPAK732VA-1 | SBI | >1 x 10^6 IFUs | EUR 826 |
|
|||
) pCDH-EF1a-Piranha-T2A-Puro virus (>1x10^6 IFUs) |
|||
| PTPD527VA-1 | SBI | >1x10^6 IFUs | EUR 697 |
 MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
|||
| CASLV120PA-KIT | SBI | 1 Kit | EUR 1132 |
|
|||
-T2A-Puro SmartNickase Lentivector Plasmid) Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid |
|||
| CASLV220PA-1 | SBI | 10 ug | EUR 697 |
|
|||
) Minicircle Dual Reporter: MSCV-RFP-T2A-Luciferase DNA (30 ug) |
|||
| BLIV506MC-1 | SBI | 30 ug | EUR 716 |
|
|||
) Minicircle Dual Reporter: MSCV-GFP-T2A-Luciferase DNA (30 ug) |
|||
| BLIV507MC-1 | SBI | 30 ug | EUR 716 |
|
|||
 pCDH-CMV-MCS-EF1-aRFP cDNA Clon/Exp Vector [pre-packaged] |
|||
| CD512VA-1 | SBI | >1 x 10^6 IFUs | EUR 786 |
|
|||
 pFC-EF1-MCS-pA-PGK-RFP-T2A-Puro PhiC31 Donor Vector |
|||
| FC550A-1 | SBI | 10ug | EUR 773 |
|
|||
 pFC-PGK-MCS-pA-EF1-GFP-T2A-Puro PhiC31 Donor Vector |
|||
| FC551A-1 | SBI | 10ug | EUR 773 |
|
|||
 pAAVK-EF1?-MCS1-CMV-MCS2 AAVanced Cloning and Expression Vector |
|||
| AAV503A-1 | SBI | 10 ug | EUR 677 |
|
|||
 pAAVK-EF1?-MCS1-CMV-EGFP AAVanced Cloning and Expression Vector |
|||
| AAV536A-1 | SBI | 10 ug | EUR 677 |
|
|||
 pAAVK-EF1?-MCS1-CMV-Puro AAVanced Cloning and Expression Vector |
|||
| AAV537A-1 | SBI | 10 ug | EUR 677 |
|
|||
 pAAVK-EF1?-MCS1-CMV-mRFP AAVanced Cloning and Expression Vector |
|||
| AAV538A-1 | SBI | 10 ug | EUR 677 |
|
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) Minicircle Dual Reporter: MSCV-RFP-T2A-Luciferase Parental Plasmid (10 ug) |
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| BLIV506MN-1 | SBI | 10 ug | EUR 972 |
|
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) Minicircle Dual Reporter: MSCV-GFP-T2A-Luciferase Parental Plasmid (10 ug) |
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| BLIV507MN-1 | SBI | 10 ug | EUR 972 |
|
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 pCDH-CMV-MCS-EF1-RFP+Puro cDNA Clon/Exp Vector [pre-packaged] |
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| CD516VB-2 | SBI | >1 x 10^6 IFUs | EUR 772 |
|
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 pCDF1-MCS2-EF1-Puro cDNA Cloning and Expression Lentivector |
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| CD110B-1 | SBI | 10 ug | EUR 635 |
|
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 MSCV-hspCas9-T2A-Puro SmartNuclease Pre-Packaged Lentiviral Particles [>10^6 IFUs] |
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| CASLV120VA-1 | SBI | 2 x 25ul | EUR 697 |
|
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) pCDH-CMV-Piranha-EF1-GFP-T2A-Puro virus (>1x10^6 IFUs) |
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| PTPD513VA-1 | SBI | >1x10^6 IFUs | EUR 697 |
 pCDH-CMV-MCS-EF1-Puro cDNA Vector cDNA Clon/Exp Vector [pre-packaged virus] |
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| CD510VB-1 | SBI | >1 x 10^6 IFUs | EUR 786 |
|
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 PCDH |
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| EF007042 | Lifescience Market | 96 Tests | EUR 689 |
PCDH |
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| EF007043 | Lifescience Market | 96 Tests | EUR 689 |
) pMC.CMV-GFP-H1-MCS (shRNA expression parental minicircle plasmid) |
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| MNSI500A-1 | SBI | 10 ug | EUR 847 |
|
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-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit) Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV220PA-KIT | SBI | 1 Kit | EUR 1132 |
|
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 MSCV-hspCas9-T2A-Puro-H1-gRNA linearized all-in-one SmartNuclease Lentivector Plasmid |
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| CASLV320PA-1 | SBI | 10 rxn | EUR 805 |
|
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) MSCV-Mouse Foxp3-EF1-GFP-T2A-Puro Stable Jurkat cell line (Puro resistant) |
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| TCL110C-1 | SBI | 5 x 10^6 cells/ml | EUR 1682 |
|
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) MSCV-Human Foxp3-EF1-GFP-T2A-Puro Stable Jurkat cell line (Puro resistant) |
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| TCL210C-1 | SBI | 5 x 10^6 cells/ml | EUR 1682 |
|
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) MSCV-Mouse RORgt-EF1-GFP-T2A-Puro Stable Jurkat cell line (Puro resistant) |
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| TCL310C-1 | SBI | 5 x 10^6 cells/ml | EUR 1682 |
|
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) MSCV-Human RORgt-EF1-GFP-T2A-Puro Stable Jurkat cell line (Puro resistant) |
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| TCL410C-1 | SBI | 5 x 10^6 cells/ml | EUR 1682 |
|
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 MSCV JMJD3 Plasmid |
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| PVT17098 | Lifescience Market | 2 ug | EUR 325 |
 XPack CMV-XP-MCS-EF1-Puro Cloning Lentivector |
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| XPAK510PA-1 | SBI | 10 ug | EUR 797 |
|
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 GFP-Fusion HR Targeting Vector [GFP-pA-LoxP-EF1?-RFP-T2A-Puro-pA-LoxP-MCS] |
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| HR120PA-1 | SBI | 10 ug | EUR 1023 |
|
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 GFP-Fusion HR Targeting Vector [GFP-pA-LoxP-EF1a-RFP-T2A-Hygro-pA-LoxP-MCS] |
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| HR220PA-1 | SBI | 10 ug | EUR 1023 |
|
|||
-T2A-Puro SmartNickase Pre-Packaged Lentiviral Particles [>10^6 IFUs]) Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Pre-Packaged Lentiviral Particles [>10^6 IFUs] |
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| CASLV220VA-1 | SBI | 2 x 25ul | EUR 697 |
|
|||
 PB-Cuo-shMCS-IRES-GFP-EF1-CymR-Puro Inducible shRNA Cloning and Expression Vector |
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| PBQMSH812A-1 | SBI | 10 ug | EUR 1096 |
|
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 pGEN- MCS |
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| PVT10565 | Lifescience Market | 2 ug | EUR 370 |
 pLEX- MCS |
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| PVT11092 | Lifescience Market | 2 ug | EUR 301 |
 MSCV-hspCas9-H1-gRNA SmartNuclease vector |
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| CAS960A-1 | SBI | 10 ug | EUR 805 |
|
|||
 PinPoint-HR attP Placement Vector ? Empty MCS for cloning HR Arms |
|||
| PIN400A-1 | SBI | 10ug | EUR 1268 |
|
|||
-T2A-Puro-H1-gRNA linearized all-in-one SmartNuclease Lentivector Plasmid) Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro-H1-gRNA linearized all-in-one SmartNuclease Lentivector Plasmid |
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| CASLV420PA-1 | SBI | 10 rxn | EUR 805 |
|
|||
 pCDH- CMV- IDH1 |
|||
| PVT10169 | Lifescience Market | 2 ug | EUR 301 |
 pCDH- CMV- CAMK2G |
|||
| PVT10240 | Lifescience Market | 2 ug | EUR 301 |
 pCDH- CMV- Rnd2 |
|||
| PVT10402 | Lifescience Market | 2 ug | EUR 301 |
 (pORF)) MCS ORF Vector (Human) (pORF) |
|||
| ORF023399 | ABM | 1.0 ug DNA | Ask for price |
 pMC.CMV-MCS-SV40polyA |
|||
| MN501A-1 | SBI | 10 ug | EUR 735 |
|
|||
 pMC.EF1-MCS-SV40polyA |
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| MN502A-1 | SBI | 10 ug | EUR 735 |
|
|||
 pMyosin-MCS-PSmOrange |
|||
| PVT17668 | Lifescience Market | 2 ug | EUR 383 |
 pECMV-MCS-Myc |
|||
| PVT17857 | Lifescience Market | 2 ug | EUR 341 |
 pLEGFP-MCS-FLAG |
|||
| PVT18164 | Lifescience Market | 2 ug | EUR 341 |
 pAAV- MCS Plasmid |
|||
| PVT2102 | Lifescience Market | 2 ug | EUR 241 |
 pT7- His- MCS |
|||
| PVT10595 | Lifescience Market | 2 ug | EUR 266 |
 pEU- E01- MCS |
|||
| PVT11379 | Lifescience Market | 2 ug | EUR 370 |
 pAAVS1-P-MCS |
|||
| PVT12296 | Lifescience Market | 2 ug | EUR 703 |
 pMD-AOX-MCS |
|||
| PVT14289 | Lifescience Market | 2 ug | EUR 911 |
 pCDNA3.1-MCS-6 |
|||
| PVT15973 | Lifescience Market | 2 ug | EUR 325 |
 pEnCMV-MCS-3 |
|||
| PVT17410 | Lifescience Market | 2 ug | EUR 300 |
) pPS-MSCV-GFP-RFP (plasmid) |
|||
| LV605PA-1 | SBI | 10 ug | EUR 656 |
|
|||
 PCDH- X/Y Antibody |
|||
| ABD8867 | Lifescience Market | 100 ug | EUR 438 |
 PCDH-X / Y Antibody |
|||
| 20-abx218014 | Abbexa |
|
|
|
|||
 PCDH-X/Y Antibody |
|||
| 45549-100ul | SAB | 100ul | EUR 252 |
PCDH-X/Y Antibody |
|||
| 45549-50ul | SAB | 50ul | EUR 187 |
PCDH-X/Y Antibody |
|||
| DF8867 | Affbiotech | 200ul | EUR 304 |
|
Description: PCDH-X/Y Antibody detects endogenous levels of total PCDH-X/Y. |
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 pCDH-513B-CTH Plasmid |
|||
| PVTB00700-4c | Lifescience Market | 2 ug | EUR 356 |
 pCDH-puro-CSF1R Plasmid |
|||
| PVTB00726-4a | Lifescience Market | 2 ug | EUR 356 |
 pCDH- puro- Bcl- XL |
|||
| PVT10290 | Lifescience Market | 2 ug | EUR 301 |
 pYLEX1 - Expression Vector |
|||
| FYY203-5MG | Yeastern Biotech | 5mg | Ask for price |
 pORB-MCS-sIRES-VSVG |
|||
| ABP-BM-sIRESVG | Allele Biotech | 5 ug | Ask for price |
|
|||
) MCS Recombinant Protein (Human) |
|||
| RP070194 | ABM | 100 ug | Ask for price |
 pCS2-EGFP-MCS-3 |
|||
| PVT17867 | Lifescience Market | 2 ug | EUR 300 |
After 58 days, ferumoxytol could be detected in HFBSCs in parts of the brain tissue. These results indicate that HFBSCs able to survive after transplantation of the brain and suggest that these cells may undergo differentiation towards neural cell lineages, which support the potential use for cell-based therapy for TBI.