Development of a novel Newcastle disease virus (NDV) neutralization test based on recombinant NDV expressing enhanced green fluorescent protein.

Development of a novel Newcastle disease virus (NDV) neutralization test based on recombinant NDV expressing enhanced green fluorescent protein.

Newcastle’s disease is one of the most important infectious diseases of poultry, caused by Newcastle Disease Virus. This virus is distributed worldwide and can lead to serious economic losses in the poultry industry due to recurring epidemics in vaccinated and unvaccinated herds. The protection against the NDV in the chickens has been associated with the development of the humoral response. Although the hemagglutination inhibition test and ELISA does not corroborate the presence of neutralizing antibodies; They are used to measure the protection and immune response against NDV.

In this study, we created a system to recover a recombinant NDV from a cloned cDNA capable of accepting exogenous genes in the desired positions. An improved green fluorescent protein (EGFP) was designed in the first position of the NDV genome and generated a recombinant. This NDV-EGFP journalist virus was used to develop a FGFP-based neutralization test, in which NABS titles were expressed as reciprocal of the highest dilution that expressed EGFP.

EGFP-NT gave conclusive results in 24 hours without using additional coloring procedure. A total of 57 serum samples were analyzed by conventional neutralization (NT) and EGFP-NT. In addition, HI and a Commercial Elisa Kit have been evaluated with the same set of samples. Although HI and ELISA showed a substantial correlation with standard NT, EGFP-NT showed a higher correlation, indicating that EGFP-NT is a method More precise to quantify NABs.

Doping of the green fluorescent protein in superfluid helium droplets: size and speed of doped droplets.

We note the doping of the green fluorescent protein from an electrospray ionization source (ESI) in superfluid helium droplets. Analyzes of temporal profiles of doped droplets, we identify two distinct groups of droplets. The faster group has a smaller average size, of the order of 106 atoms / droplets of helium, and the slower group is much larger, by at least one order of magnitude. The relative populations of these two groups depend on the temperature of the source droplet: from 11 to 5 K, the signal intensity of the group of lower droplets gradually increases, closely from the detection limit to that of the faster group.

We post that the smaller droplets are formed via the condensation of gaseous helium during the expansion of the pulsed valve, while the larger droplets are developing the fragmentation of the ejected liquid helium. Our results on the size and speed of the condensing peak at temperatures above the source (> 7 k) agrees with the previous reports, but those at lower temperatures (<7 K) appear to be extinguished. We assign this divergence to the masking effect of extremely large droplets of the fragmentary peak in previous measurements of droplet sizes. In the temperature range of our survey, although the expansion requirement changes from supercritical subcriture, there is no steep modification of the distribution of the speed or distribution of the condensation peak, and L The most salient effect is in the growing intensity of fragmentation. peak.

The effectiveness of absolute doping, as expressed by the ratio of droplets doped ions on the total number of ions from the ESI source, is of the order of 10 to 4, while only hundreds of ions. doped have been detected. Other improvements in the ESI source are essential to prolong the technology of future experiences. On the other hand, the separation of the two groups of speed droplets is beneficial for selecting the size of only smaller droplets for future electronic diffraction experiments.

 Development of a novel Newcastle disease virus (NDV) neutralization test based on recombinant NDV expressing enhanced green fluorescent protein.
Development of a novel Newcastle disease virus (NDV) neutralization test based on recombinant NDV expressing enhanced green fluorescent protein.

An unisleascent correlation between the dynamics of the chromophore chromophore pocket of green fluorescent proteins and hydrogen: a choreography of the Molecular Dynamics AB initio

The green fluorescent protein (GFP) is a chemical system largely studied for both its large amount of applications and the complexity of the proton transfer of the excited state responsible for the variation of the protection state of the chromophore. A detailed investigation into the structure of the chromophore environment and the influence of the chromophore (neutral or anionic) form is of crucial importance to understand how these factors could potentially influence protein function. In this study, we carry out a detailed computer inquiry based on the analysis of the simulations of molecular dynamics ab-initio, in order to unravel the main structural quantities determining the fine balance in the chromic environment.

We have found that specific hydrogen-related interactions directly involving the chromophore (or not), are correlated to quantities, such as the volume of the cavity in which the chromophore is integrated and importantly important by the State of the Cartenariat of the Chromophore. The cross-sectional correlation analysis carried out on some of these hydrogen links and the volume of the cavity, demonstrates a direct correlation between them and we have also identified those specifically involved in this correlation. We also found that specific interactions between residues by far in space are correlated, demonstrating the complexity of the chromophore environment and that many structural amounts must be taken into account to describe and understand the main factors to settle.

mCherry (EF1a, Puro), Concentrated Lentivirus

LVP1420-PBS 1x10^8 IFU/ml x 200ul
EUR 455
Description: mCherry expression lentivirus under EF1a promoter, carrying Puromycin selection

pCDH- CMV- MCS- EF1- puro

PVT11075 2 ug
EUR 444

pGreen- Puro- pSIH1- H1- copGFP- T2A- Puro

PVT11116 2 ug
EUR 444

pCDH- CMV- MCS- EF1- turboRFP- T2A- Puro

PVT11077 2 ug
EUR 444

pCDH-CMV-Akaluc-EF1-CopGFP-T2A-Puro

PVT14490 2 ug
EUR 718.8

pAAV- CMV- mCherry- U6- sgRNA

PVT11046 2 ug
EUR 361.2

pCDNA3.1- mCherry

PVT10770 2 ug
EUR 319.2

pCDH-CMV-ccl3-m-EF1-CopGFP-T2A-Puro

PVT19054 2 ug
EUR 360

pCDH-CMV-efemp1-m-EF1-CopGFP-T2A-Puro

PVT17438 2 ug
EUR 360

pCDH-CMV-TXNL1-HA-EF1-CopGFP-T2A-Puro

PVT17693 2 ug
EUR 309.6

pCDH-CMV-MCS-EF1-copGFP-T2A-Puro plasmid

PVT17271 2 ug
EUR 568.8

pCDH-CMV-CCDC68-HA-EF1-CopGFP-T2A-Puro

PVT17760 2 ug
EUR 309.6

pCDH-CMV-PTX3-EF1-CopGFP-T2A-Puro Plasmid

PVT17116 2 ug
EUR 390

pCDH-CMV-GSTA1-EF1-copGFP Plasmid

PVT16093 2 ug
EUR 390

pCDH-CMV-exosome booster-EF1-copGFP-Puro vector

PVT12109 2 ug
EUR 1149.6

pHG52d34.5-CMV-PD-1(b)-mCherry

PVT12169 2 ug
EUR 843.6

mCherry

MO22192 100 ul
EUR 522

mCherry

GT22108 each
EUR 272.7

pBABE-puro mCherry-EGFP-LC3B

PVT14018 2 ug
EUR 1093.2

pCDH-CMV-MCS-EF1-copGFP+Puro cDNA Clon/Exp Vector [pre-packaged]

CD513VB-1 >1 x 10^6 IFUs
EUR 720

pT2- mCherry

PVT10881 2 ug
EUR 361.2

pLV- mCherry

PVT11094 2 ug
EUR 361.2

mCherry - Mouse

MO22140 100 ul
EUR 522

pDawn- mCherry

PVT10619 2 ug
EUR 361.2

pUC35s- mCherry

PVT11192 2 ug
EUR 319.2

pET28a- mCherry

PVT0075 2 ug
EUR 307.2

pNanog- mCherry

PVT10426 2 ug
EUR 319.2

pBV220- mCherry

PVT10620 2 ug
EUR 361.2

pRSETB- mCherry

PVT10624 2 ug
EUR 361.2

mCherry Antibody

5993-100 each
EUR 483.6

mCherry Antibody

5993-30T each
EUR 175.2

mCherry Antibody

abx332840-100ul 100 ul
EUR 510

mCherry antibody

70R-12458 100 ug
EUR 490
Description: Rabbit polyclonal mCherry antibody

mCherry Antibody

20-abx159607
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  • 50 ug

mCherry-Rabbit

RA22117 100 ul
EUR 522

mCherry Antibody

abx159607-100g 100 µg Ask for price

mCherry Antibody

abx159607-10g 10 µg
EUR 187.5

mCherry Antibody

abx159607-50g 50 µg
EUR 250

mCherry Antibody

abx375280-96tests 96 tests
EUR 337.5

mCherry Antibody

abx332840-100g 100 µg Ask for price

mCherry Antibody

abx332840-20g 20 µg
EUR 362.5

mCherry Antibody

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mCherry Antibody

abx347160-100g 100 µg Ask for price

mCherry Antibody

abx347160-20g 20 µg
EUR 350

mCherry Antibody

abx347160-50g 50 µg Ask for price

pCDH-EF1-Luc-T2A-copGFP

PVT17649 2 ug
EUR 508.8

mCherry-Chicken

CH22115 100 ul
EUR 522

pCDH- EF1- MCS- T2A- Puro Plasmid

PVT2335 2 ug
EUR 390

PA- mCherry- C1

PVT10759 2 ug
EUR 361.2

PA- mCherry- N1

PVT10760 2 ug
EUR 361.2

pLVX- mCherry- N1

PVT11067 2 ug
EUR 361.2

pLVX- mCherry- C1

PVT11068 2 ug
EUR 444

pUC57- Tac- mCherry

PVT10621 2 ug
EUR 444

pCAS9- mCherry empty

PVT10985 2 ug
EUR 319.2

mCherry tag Antibody

abx019132-100ug 100 ug
EUR 427.2

mCherry tag Antibody

abx019132-400l 400 µl Ask for price

mCherry tag Antibody

abx019132-80l 80 µl
EUR 287.5

pBBR1MCS2- Tac- mCherry

PVT11376 2 ug
EUR 444

pUC57- mCherry Plasmid

PVT0008 2 ug
EUR 390

mCherry-Tag Antibody

1-CSB-PA000343
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  • 100ug
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Description: A polyclonal antibody against mCherry-Tag. Recognizes mCherry-Tag from . This antibody is Unconjugated. Tested in the following application: WB;WB:1:5000

mCherry-tag Antibody

E38PA9033 100ul
EUR 225
Description: Available in various conjugation types.

mCherry-tag Antibody

E38PA9034 100ul
EUR 225
Description: Available in various conjugation types.

mCherry-tag Antibody

E38PA9061 100ul
EUR 225
Description: Available in various conjugation types.

mCherry-Tag Antibody

20-abx330249
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  • 100 ug
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mCherry-Tag Antibody

20-abx330282
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  • 100 ug
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mCherry-Tag Antibody

20-abx242885
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mCherry-tag Antibody

20-abx134448
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20-abx134449
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  • 100 ul
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20-abx134450
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  • 100 ul
  • 200 ul
  • 30 ul

pBI121- mCherry Plasmid

PVT3005 2 ug
EUR 289.2

pET28a- mCherry Plasmid

PVT0334 2 ug
EUR 307.2

mCherry-Tag Antibody

T0090 1ml
EUR 420

mCherry-Tag Antibody

T0090-100ul 100ul
EUR 280

mCherry-Tag Antibody

T0090-200ul 200ul
EUR 350

mCherry-Tag Antibody

abx242885-96tests 96 tests
EUR 200

mCherry-Tag Antibody

abx330249-100g 100 µg Ask for price

mCherry-Tag Antibody

abx330249-20g 20 µg
EUR 187.5

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abx330249-50g 50 µg
EUR 250

mCherry-Tag Antibody

abx330282-100g 100 µg Ask for price

mCherry-Tag Antibody

abx330282-20g 20 µg
EUR 187.5

mCherry-Tag Antibody

abx330282-50g 50 µg
EUR 250

pBARGPE1- mCherry Plasmid

PVT5308 2 ug
EUR 390

mCherry Mouse mAb (6F7)

E2250171 100ul
EUR 225
Description: Available in various conjugation types.

pHDE- 35s- Cas9- mCherry

PVT11215 2 ug
EUR 361.2

mCherry- Tubulin- C- 18

PVT10163 2 ug
EUR 361.2

pUC35s- RAT5- a- mCherry

PVT11193 2 ug
EUR 361.2

pTriEx- mCherry- PA- Rac1

PVT10328 2 ug
EUR 319.2

mCherry Quantification Kit

K971-100 each
EUR 477.6

mCherry Monoclonal Antibody

E20-53125 100ug
EUR 225
Description: Available in various conjugation types.

mCherry Fluorescent Protein

4993-100 each
EUR 489.6

mCherry Fluorescent Protein

4993-1000 each
EUR 3145.2

mCherry Fluorescent Protein

4993-5000 each
EUR 9175.2

mCherry Monoclonal Antibody

ABM40125-003ml 0.03ml
EUR 189.6
Description: A monoclonal antibody for detection of mCherry from Null. This mCherry antibody is for WB. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen recombinant protein

mCherry Monoclonal Antibody

ABM40125-01ml 0.1ml
EUR 346.8
Description: A monoclonal antibody for detection of mCherry from Null. This mCherry antibody is for WB. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen recombinant protein

mCherry Monoclonal Antibody

ABM40125-02ml 0.2ml
EUR 496.8
Description: A monoclonal antibody for detection of mCherry from Null. This mCherry antibody is for WB. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen recombinant protein

mCherry Monoclonal Antibody

ABM40125-100uL 100 μL
EUR 239
Description: Mouse Anti-mCherry Monoclonal Antibody

mCherry Monoclonal Antibody

ABM40125-200uL 200 μL
EUR 379
Description: Mouse Anti-mCherry Monoclonal Antibody

mCherry Monoclonal Antibody

ABM40125-30uL 30 μL
EUR 109
Description: Mouse Anti-mCherry Monoclonal Antibody

mCherry-sephluorin-ATP9

PVT17635 2 ug
EUR 409.2

mCherry Monoclonal Antibody

E-AB-20087-120uL 120uL
EUR 178
Description: Unconjugated

mCherry Monoclonal Antibody

E-AB-20087-200uL 200uL
EUR 209
Description: Unconjugated

mCherry Monoclonal Antibody

E-AB-20087-30uL 30uL
EUR 73
Description: Unconjugated

mCherry Monoclonal Antibody

E-AB-20087-60uL 60uL
EUR 104
Description: Unconjugated

pLVX- IRES- mCherry Plasmid

PVT2307 2 ug
EUR 390

mCherry mAntibody (Clone 1)

5411-100 each
EUR 464.4

NL4-3 mCherry Luciferase

PVT17706 2 ug
EUR 360

Mouse antibody for mCherry

SPC-1268D-A565 0.1ml
EUR 432
Description: A polyclonal antibody for mCherry from Species Independent. The antibody is produced in Mouse after immunization with Anaplasma marginale Full-length protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This mCherry antibody is conjugated to ATTO 565.

Mouse antibody for mCherry

SPC-1268D-A633 0.1ml
EUR 432
Description: A polyclonal antibody for mCherry from Species Independent. The antibody is produced in Mouse after immunization with Anaplasma marginale Full-length protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This mCherry antibody is conjugated to ATTO 633.

Mouse antibody for mCherry

SPC-1268D-A655 0.1ml
EUR 432
Description: A polyclonal antibody for mCherry from Species Independent. The antibody is produced in Mouse after immunization with Anaplasma marginale Full-length protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This mCherry antibody is conjugated to ATTO 655.

Mouse antibody for mCherry

SPC-1268D-A680 0.1ml
EUR 432
Description: A polyclonal antibody for mCherry from Species Independent. The antibody is produced in Mouse after immunization with Anaplasma marginale Full-length protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This mCherry antibody is conjugated to ATTO 680.

Mouse antibody for mCherry

SPC-1268D-A700 0.1ml
EUR 432
Description: A polyclonal antibody for mCherry from Species Independent. The antibody is produced in Mouse after immunization with Anaplasma marginale Full-length protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This mCherry antibody is conjugated to ATTO 700.

Mouse antibody for mCherry

SPC-1268D-ALP 0.1ml
EUR 426
Description: A polyclonal antibody for mCherry from Species Independent. The antibody is produced in Mouse after immunization with Anaplasma marginale Full-length protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This mCherry antibody is conjugated to Alkaline Phosphatase.

Mouse antibody for mCherry

SPC-1268D-APC 0.1ml
EUR 430.8
Description: A polyclonal antibody for mCherry from Species Independent. The antibody is produced in Mouse after immunization with Anaplasma marginale Full-length protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This mCherry antibody is conjugated to APC .

Mouse antibody for mCherry

SPC-1268D-APCCY7 0.1ml
EUR 518.4
Description: A polyclonal antibody for mCherry from Species Independent. The antibody is produced in Mouse after immunization with Anaplasma marginale Full-length protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This mCherry antibody is conjugated to APC/Cy7.

Mouse antibody for mCherry

SPC-1268D-DY350 0.1ml
EUR 523.2
Description: A polyclonal antibody for mCherry from Species Independent. The antibody is produced in Mouse after immunization with Anaplasma marginale Full-length protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This mCherry antibody is conjugated to Dylight 350.

Mouse antibody for mCherry

SPC-1268D-DY405 0.1ml
EUR 494.4
Description: A polyclonal antibody for mCherry from Species Independent. The antibody is produced in Mouse after immunization with Anaplasma marginale Full-length protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This mCherry antibody is conjugated to Dylight 405.

Mouse antibody for mCherry

SPC-1268D-DY488 0.1ml
EUR 471.6
Description: A polyclonal antibody for mCherry from Species Independent. The antibody is produced in Mouse after immunization with Anaplasma marginale Full-length protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This mCherry antibody is conjugated to Dylight 488.

Mouse antibody for mCherry

SPC-1268D-DY594 0.1ml
EUR 476.4
Description: A polyclonal antibody for mCherry from Species Independent. The antibody is produced in Mouse after immunization with Anaplasma marginale Full-length protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This mCherry antibody is conjugated to Dylight 594.

Mouse antibody for mCherry

SPC-1268D-DY633 0.1ml
EUR 464.4
Description: A polyclonal antibody for mCherry from Species Independent. The antibody is produced in Mouse after immunization with Anaplasma marginale Full-length protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This mCherry antibody is conjugated to Dylight 633.

Mouse antibody for mCherry

SPC-1268D-HRP 0.1ml
EUR 418.8
Description: A polyclonal antibody for mCherry from Species Independent. The antibody is produced in Mouse after immunization with Anaplasma marginale Full-length protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This mCherry antibody is conjugated to HRP.

Mouse antibody for mCherry

SPC-1268D-P594 0.1ml
EUR 440.4
Description: A polyclonal antibody for mCherry from Species Independent. The antibody is produced in Mouse after immunization with Anaplasma marginale Full-length protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This mCherry antibody is conjugated to PE/ATTO 594.

Mouse antibody for mCherry

SPC-1268D-PCP 0.1ml
EUR 430.8
Description: A polyclonal antibody for mCherry from Species Independent. The antibody is produced in Mouse after immunization with Anaplasma marginale Full-length protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This mCherry antibody is conjugated to PerCP.

Mouse antibody for mCherry

SPC-1268D-RPE 0.1ml
EUR 429.6
Description: A polyclonal antibody for mCherry from Species Independent. The antibody is produced in Mouse after immunization with Anaplasma marginale Full-length protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This mCherry antibody is conjugated to RPE .

Mouse antibody for mCherry

SPC-1268D-STR 0.1ml
EUR 430.8
Description: A polyclonal antibody for mCherry from Species Independent. The antibody is produced in Mouse after immunization with Anaplasma marginale Full-length protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This mCherry antibody is conjugated to Streptavidin.

The active site of the protein. Of an overall assessment of the results obtained in this work, it is shown that the residues that the spectators are perceived as spectators play both an important role in the influence on the chromophore environment (volume of the cavity) and its dynamic (crossed correlations between them. Remote residues)

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