Development of a novel Newcastle disease virus (NDV) neutralization test based on recombinant NDV expressing enhanced green fluorescent protein.

Development of a novel Newcastle disease virus (NDV) neutralization test based on recombinant NDV expressing enhanced green fluorescent protein.

Newcastle’s disease is one of the most important infectious diseases of poultry, caused by Newcastle Disease Virus. This virus is distributed worldwide and can lead to serious economic losses in the poultry industry due to recurring epidemics in vaccinated and unvaccinated herds. The protection against the NDV in the chickens has been associated with the development of the humoral response. Although the hemagglutination inhibition test and ELISA does not corroborate the presence of neutralizing antibodies; They are used to measure the protection and immune response against NDV.

In this study, we created a system to recover a recombinant NDV from a cloned cDNA capable of accepting exogenous genes in the desired positions. An improved green fluorescent protein (EGFP) was designed in the first position of the NDV genome and generated a recombinant. This NDV-EGFP journalist virus was used to develop a FGFP-based neutralization test, in which NABS titles were expressed as reciprocal of the highest dilution that expressed EGFP.

EGFP-NT gave conclusive results in 24 hours without using additional coloring procedure. A total of 57 serum samples were analyzed by conventional neutralization (NT) and EGFP-NT. In addition, HI and a Commercial Elisa Kit have been evaluated with the same set of samples. Although HI and ELISA showed a substantial correlation with standard NT, EGFP-NT showed a higher correlation, indicating that EGFP-NT is a method More precise to quantify NABs.

Doping of the green fluorescent protein in superfluid helium droplets: size and speed of doped droplets.

We note the doping of the green fluorescent protein from an electrospray ionization source (ESI) in superfluid helium droplets. Analyzes of temporal profiles of doped droplets, we identify two distinct groups of droplets. The faster group has a smaller average size, of the order of 106 atoms / droplets of helium, and the slower group is much larger, by at least one order of magnitude. The relative populations of these two groups depend on the temperature of the source droplet: from 11 to 5 K, the signal intensity of the group of lower droplets gradually increases, closely from the detection limit to that of the faster group.

We post that the smaller droplets are formed via the condensation of gaseous helium during the expansion of the pulsed valve, while the larger droplets are developing the fragmentation of the ejected liquid helium. Our results on the size and speed of the condensing peak at temperatures above the source (> 7 k) agrees with the previous reports, but those at lower temperatures (<7 K) appear to be extinguished. We assign this divergence to the masking effect of extremely large droplets of the fragmentary peak in previous measurements of droplet sizes. In the temperature range of our survey, although the expansion requirement changes from supercritical subcriture, there is no steep modification of the distribution of the speed or distribution of the condensation peak, and L The most salient effect is in the growing intensity of fragmentation. peak.

The effectiveness of absolute doping, as expressed by the ratio of droplets doped ions on the total number of ions from the ESI source, is of the order of 10 to 4, while only hundreds of ions. doped have been detected. Other improvements in the ESI source are essential to prolong the technology of future experiences. On the other hand, the separation of the two groups of speed droplets is beneficial for selecting the size of only smaller droplets for future electronic diffraction experiments.

 Development of a novel Newcastle disease virus (NDV) neutralization test based on recombinant NDV expressing enhanced green fluorescent protein.
Development of a novel Newcastle disease virus (NDV) neutralization test based on recombinant NDV expressing enhanced green fluorescent protein.

An unisleascent correlation between the dynamics of the chromophore chromophore pocket of green fluorescent proteins and hydrogen: a choreography of the Molecular Dynamics AB initio

The green fluorescent protein (GFP) is a chemical system largely studied for both its large amount of applications and the complexity of the proton transfer of the excited state responsible for the variation of the protection state of the chromophore. A detailed investigation into the structure of the chromophore environment and the influence of the chromophore (neutral or anionic) form is of crucial importance to understand how these factors could potentially influence protein function. In this study, we carry out a detailed computer inquiry based on the analysis of the simulations of molecular dynamics ab-initio, in order to unravel the main structural quantities determining the fine balance in the chromic environment.

We have found that specific hydrogen-related interactions directly involving the chromophore (or not), are correlated to quantities, such as the volume of the cavity in which the chromophore is integrated and importantly important by the State of the Cartenariat of the Chromophore. The cross-sectional correlation analysis carried out on some of these hydrogen links and the volume of the cavity, demonstrates a direct correlation between them and we have also identified those specifically involved in this correlation. We also found that specific interactions between residues by far in space are correlated, demonstrating the complexity of the chromophore environment and that many structural amounts must be taken into account to describe and understand the main factors to settle.

pCDH- CMV- MCS- EF1- copGFP Plasmid

PVT2334 2 ug
EUR 325

pCDH-CMV-GSTA1-EF1-copGFP Plasmid

PVT16093 2 ug
EUR 325

pCDH-CMV-TXNL1-HA-EF1-CopGFP-T2A-Puro

PVT17693 2 ug
EUR 258

pCDH-CMV-CCDC68-HA-EF1-CopGFP-T2A-Puro

PVT17760 2 ug
EUR 258

pCDH-CMV-ccl3-m-EF1-CopGFP-T2A-Puro

PVT19054 2 ug
EUR 300

pCDH-CMV-exosome booster-EF1-copGFP-Puro vector

PVT12109 2 ug
EUR 958

pCDH-CMV-PTX3-EF1-CopGFP-T2A-Puro Plasmid

PVT17116 2 ug
EUR 325

pCDH-CMV-MCS-EF1-copGFP-T2A-Puro plasmid

PVT17271 2 ug
EUR 474

pCDH-CMV-efemp1-m-EF1-CopGFP-T2A-Puro

PVT17438 2 ug
EUR 300

pCDH-EF1-Luc-T2A-copGFP

PVT17649 2 ug
EUR 424

pCDH- EF1- MCS- T2A- copGFP

PVT2336 2 ug
EUR 325

pCDH- MSCV- MCS- EF1- CopGFP- T2A- Puro

PVT11079 2 ug
EUR 370

pCDH- CMV- MCS- EF1- puro

PVT11075 2 ug
EUR 370

pCDH-CMV-MCS-EF1-copGFP+Puro cDNA Clon/Exp Vector [pre-packaged]

CD513VB-1 >1 x 10^6 IFUs
EUR 786
  • Category: Lentiviral Technology

pCDH-CMV-MCS-EF1-copGFP cDNA Cloning and Expression Vector

CD511B-1 10 ug
EUR 635
  • Category: Lentiviral Technology

pCDH-CMV-Bach2-m-EF1-Puro

PVT13474 2 ug
EUR 703

pCDH-CMV-FGL1-EF1-Puro Plasmid

PVT16069 2 ug
EUR 325

pCDH-CMV-LAG3-EF1-Puro Plasmid

PVT16070 2 ug
EUR 325

Scramble control hairpin in pCDH-CMV-MCS-EF1-copGFP (CD511B-1)

PMIRH000PA-1 Bacterial Streak
EUR 656
  • Category: MicroRNA Tools

pCDH- CMV- MCS- EF1- turboRFP- T2A- Puro

PVT11077 2 ug
EUR 370

pCDH-CMV-3-FLAG-TOX3-EF1-Puro

PVT13401 2 ug
EUR 703

pCDH-CMV-GLUT1-EF1-turboRFP-T2A-Puro

PVT13588 2 ug
EUR 599

pCDH-CMV-mRFP1-EGFP-LC3B-EF1-Puro

PVT14019 2 ug
EUR 911

pCDH-CMV-MCS-EF1-Puro (CD510B) plasmid

PVT17272 2 ug
EUR 474

pCDH-CMV-MCS-EF1-copGFP cDNA Clon/Exp Vector [pre-packaged virus]

CD511VB-1 >1 x 10^6 IFUs
EUR 786
  • Category: Lentiviral Technology

pCDH- CMV- MCS- EF1- Hygro

PVT11076 2 ug
EUR 370

CMV Control lentiviral particles (Puro)

CMV-Null-Puro 1 x107 IFU/ml x 200ul
EUR 349
Description: Negative control lentivirus contains a null spacer insert under CMV promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It also has the Puromycin marker under RSV promoter.

pCDH-CMV-MCS-EF1-Puro-FLAG-HIF2 alpha

PVT12166 2 ug
EUR 370

pCDH- EF1- MCS- T2A- Puro Plasmid

PVT2335 2 ug
EUR 325

pCDH-EF1-CymR-T2A-Puro (plasmid)

QM200PA-2 10 ug
EUR 597
  • Category: Lentiviral Technology

pCDH-EF1-CymR-T2A-Puro (virus)

QM200VA-2 >1 x 10^6 IFUs
EUR 636
  • Category: Lentiviral Technology

pCDH-CMV-exosome booster-EF1-Hygro

PVT12359 2 ug
EUR 2991

pCDH-CMV-PDCD1-EF1-Hygro Plasmid

PVT16072 2 ug
EUR 325

pCDH-CMV-MCS-EF1-EGFP-IRFP

PVT17586 2 ug
EUR 424

pCDH-Cuo-MCS-EF1-CymR-T2A-Puro

QM800A-1 10 ug
EUR 1310
  • Category: Lentiviral Technology

pCDH-EF1-MCS-T2A-Puro (CD520A) plasmid

PVT17273 2 ug
EUR 474

pCDH-CMV-MCS-EF1-copGFP cDNA Clon/Exp Vector [pre-packaged Ultra-High Titer virus]

CD511VB-UHT >1x10^9 IFUs
EUR 1041
  • Category: Lentiviral Technology

pCDH-CMV-MCS-EF1-Puro cDNA Cloning and Expression Vector

CD510B-1 10 ug
EUR 635
  • Category: Lentiviral Technology

pCDH-EF1-MCS*-T2A-GFP

CD526A-1 10 ug
EUR 653
  • Category: Lentiviral Technology

pCDH-EF1-Luc2-T2A-ZsGreen1

PVT17917 2 ug
EUR 424

pLVX-EF1伪-IRES-mCherry

PVT2309 2 ug
EUR 325

CMV Control lentiviral particles (Puro) in PBS

CMV-Null-Puro-PBS 1 x108 IFU/ml x 200ul
EUR 710
Description: Negative control lentivirus contains a null spacer insert under CMV promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It also has the Puromycin marker under RSV promoter. The virus was concentrated and provided in PBS solution.

pCDH-CMV-MCS-EF1-RFP+Puro cDNA Cloning and Expression Vector

CD516B-2 10 ug
EUR 635
  • Category: Lentiviral Technology

pCDH-CMV-Piranha-EF1-GFP-T2A-Puro virus (>1x10^6 IFUs)

PTPD513VA-1 >1x10^6 IFUs
EUR 697

pCDH-CMV-MCS-t2A-copGFP cDNA Cloning and Expression Vector

CD524A-1 10 ug
EUR 653
  • Category: Lentiviral Technology

pCDH-EF1-MCS-(PGK-Puro) Cloning and Expression Vector

CD810A-1 10 ug
EUR 642
  • Category: Lentiviral Technology

pCDH- EF1- luc2- T2A- Tdtomato Plasmid

PVT2337 2 ug
EUR 241

pCDH-EF1-CymR-T2A-RFP (plasmid)

QM300PA-1 10 ug
EUR 597
  • Category: Lentiviral Technology

pCDH-EF1-CymR-T2A-RFP (virus)

QM300VA-1 >1 x 10^6 IFUs
EUR 636
  • Category: Lentiviral Technology

pCDH-EF1-CymR-T2A-Neo (plasmid)

QM400PA-2 10 ug
EUR 581
  • Category: Lentiviral Technology

pCDH-EF1-CymR-T2A-Neo (virus)

QM400VA-2 >1 x 10^6 IFUs
EUR 620
  • Category: Lentiviral Technology

pCDH- ECMV- MCS- 3*Flag- EF1

PVT11080 2 ug
EUR 370

pCDH-CMV-MCS-EF1-RFP+Puro cDNA Clon/Exp Vector [pre-packaged]

CD516VB-2 >1 x 10^6 IFUs
EUR 772
  • Category: Lentiviral Technology

pCDH-EF1-MCS-IRES-Puro cDNA Cloning and Expression Vector

CD532A-2 10 ug
EUR 653
  • Category: Lentiviral Technology

pCDH-MSCV-MCS-EF1-Puro cDNA Cloning and Expression Vector

CD710B-1 10 ug
EUR 635
  • Category: Lentiviral Technology

pCDH-MCS-T2A-copGFP-MSCV Cloning and Expression Vector

CD523A-1 10 ug
EUR 653
  • Category: Lentiviral Technology

pCDH-CMV-MCS-EF1-RFP cDNA Cloning and Expression Vector

CD512B-1 10 ug
EUR 635
  • Category: Lentiviral Technology

pCDH-CMV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector

CD513B-1 10 ug
EUR 635
  • Category: Lentiviral Technology

pCDH-CMV-MCS-EF1-Neo cDNA Cloning and Expression Vector

CD514B-1 10 ug
EUR 635
  • Category: Lentiviral Technology

pCDH-CMV-MCS-EF1-Hygro cDNA Cloning and Expression Vector

CD515B-1 10 ug
EUR 635
  • Category: Lentiviral Technology

hTERT (CMV, Puro) lentivirus

LVP1130-Puro 1x107 IFU/ml x 200ul
EUR 349
Description: Premade lentivirus expressing human TERT gene under suCMV promoter, containing Puromycin marker.

pCDH-MSCV-MCS-EF1-GFP+Puro cDNA Cloning and Expression Vector

CD713B-1 10 ug
EUR 635
  • Category: Lentiviral Technology

pCDH-EF1-MCS-(PGK-GFP-T2A-Puro) Cloning and Expression Vector

CD813A-1 10 ug
EUR 642
  • Category: Lentiviral Technology

pCDH-EF1-MCS-T2A-RFP (PGK-Puro) Cloning and Expression Vector

CD822A-1 10 ug
EUR 642
  • Category: Lentiviral Technology

pCDH-EF1-MCS-T2A-GFP (PGK-Puro) Cloning and Expression Vector

CD823A-1 10 ug
EUR 642
  • Category: Lentiviral Technology

pCDH-CMV-MCS-EF1-Puro cDNA Vector cDNA Clon/Exp Vector [pre-packaged virus]

CD510VB-1 >1 x 10^6 IFUs
EUR 786
  • Category: Lentiviral Technology

pCDF1-MCS2-EF1-copGFP cDNA Cloning and Expression Vector

CD111B-1 10 ug
EUR 635
  • Category: Lentiviral Technology

CMV-TetR (Puro) Lentiviral particles 

LVP017-Puro 1x107 IFU/ml x 200ul
EUR 404
Description: Pre-made lentiviral particles expressing tetracycline regulator protein (TetR) under CMV promoter, with Puromycin antibiotic marker.

pCDH-CMV-MCS-EF1-aRFP cDNA Clon/Exp Vector [pre-packaged]

CD512VA-1 >1 x 10^6 IFUs
EUR 786
  • Category: Lentiviral Technology

pCDH-EF1-MCS cDNA Cloning and Expression Vector

CD502A-1 10 ug
EUR 544
  • Category: Lentiviral Technology

pCDH-CuO-MCS-EF1-GFP dual promoter lentivector

QM511B-1 10 ug
EUR 679
  • Category: Lentiviral Technology

pCDH-CuO-MCS-EF1-RFP dual promoter lentivector

QM512B-1 10 ug
EUR 679
  • Category: Lentiviral Technology

pCDH-EF1-MCS-T2A-Puro cDNA Cloning and Expression Vector 10 ug

CD527A-1 10 ug
EUR 653
  • Category: Lentiviral Technology

pCDH-CuO-MCS-EF1-GFP+Puro dual promoter and dual marker lentivector

QM513B-1 10 ug
EUR 679
  • Category: Lentiviral Technology

pCDH-CuO-MCS-EF1-RFP+Puro dual promoter and dual marker lentivector

QM516B-2 10 ug
EUR 679
  • Category: Lentiviral Technology

pCDH- CMV- IDH1

PVT10169 2 ug
EUR 301

pCDH- CMV- CAMK2G

PVT10240 2 ug
EUR 301

pCDH- CMV- Rnd2

PVT10402 2 ug
EUR 301

pCDH-EF1-MCS-(PGK-GFP) Cloning and Expression Vector

CD811A-1 10 ug
EUR 642
  • Category: Lentiviral Technology

pCDH-puro-CSF1R Plasmid

PVTB00726-4a 2 ug
EUR 356

pCDH- puro- Bcl- XL

PVT10290 2 ug
EUR 301

pCDH-EF1-MCS-BGH-PGK-GFP-T2A-Puro cDNA Cloning and Expression Vector

CD550A-1 10 ug
EUR 625
  • Category: Lentiviral Technology

SV40 large T-antigen (Puro), CMV lentiviral particles

LVP016-Puro 1x107 IFU/ml x 200ul
EUR 349
Description: Pre-made lentiviral particles expressing SV40 large T-antigen under suCMV promoter, containing puromycin marker.

pGreen- Puro- pSIH1- H1- copGFP- T2A- Puro

PVT11116 2 ug
EUR 370

CMV Control lentiviral particles (GFP-Puro)

CMV-Null-GP 1 x107 IFU/ml x 200ul
EUR 349
Description: Negative control lentivirus contains a null spacer insert under CMV promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It also has the GFP-Puromycin fusion marker under RSV promoter.

CMV Control lentiviral particles (RFP-Puro)

CMV-Null-RP 1 x107 IFU/ml x 200ul
EUR 349
Description: Negative control lentivirus contains a null spacer insert under CMV promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It also has the RFP-Puromycin fusion marker under RSV promoter.

pCDH-EF1-MCS-IRES-GFP cDNA Cloning and Expression Vector

CD530A-2 10 ug
EUR 653
  • Category: Lentiviral Technology

pCDH-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector

CD531A-2 10 ug
EUR 653
  • Category: Lentiviral Technology

pCDH-EF1-MCS-IRES-Neo cDNA Cloning and Expression Vector

CD533A-2 10 ug
EUR 653
  • Category: Lentiviral Technology

pCDH-UbC-MCS-EF1-Hygro cDNA Cloning and Expression Vector

CD615B-1 10 ug
EUR 635
  • Category: Lentiviral Technology

pCDH-MSCV-MCS-EF1-GFP cDNA Cloning and Expression Vector

CD711B-1 10 ug
EUR 635
  • Category: Lentiviral Technology

pCDH-puro-CSF1R(P855T) Plasmid

PVTB00726-4b 2 ug
EUR 356

hTERT (CMV, Puro) lentivirus in PBS

LVP1130-Puro-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Concentrated lentivirus in PBS, expressing human TERT gene under suCMV promoter, containing Puromycin marker.

PCDH

EF007042 96 Tests
EUR 689

PCDH

EF007043 96 Tests
EUR 689

pBABE-puro mCherry-EGFP-LC3B

PVT14018 2 ug
EUR 911

pCDH-CuO-MCS-IRES-GFP-EF1-CymR-T2A-Puro All-in-one inducible IRES lentivector

QM812B-1 10 ug
EUR 1310
  • Category: Lentiviral Technology

XPack CMV-XP-MCS-EF1-Puro Cloning Lentivector

XPAK510PA-1 10 ug
EUR 797
  • Category: Exosome

XPack CMV-XP-GFP-EF1-Puro Expression Lentivector

XPAK530PA-1 10 ug
EUR 826
  • Category: Exosome

XPack CMV-XP-GFP-EF1-Puro Expression Lentivirus

XPAK530VA-1 >1 x 10^6 IFUs
EUR 826
  • Category: Exosome

XPack CMV-XP-RFP-EF1-Puro Expression Lentivector

XPAK531PA-1 10 ug
EUR 826
  • Category: Exosome

XPack CMV-XP-Luciferase-EF1-Puro Expression Lentivector

XPAK532PA-1 10 ug
EUR 826
  • Category: Exosome

XPack CMV-XP-Luciferase-EF1-Puro Expression Lentivirus

XPAK532VA-1 >1 x 10^6 IFUs
EUR 826
  • Category: Exosome

mCherry

MO22192 100 ul
EUR 435

CMV-TetR (Puro) Lentiviral particles  in PBS

LVP017-Puro-PBS 5x107 IFU/ml x 200ul
EUR 507
Description: Pre-made lentiviral particles expressing tetracycline regulator protein (TetR) under CMV promoter, with Puromycin antibiotic marker. Particles pellet was re-suspended in PBS solution.

HEK293-TetR (Puro)

SC005-Puro 2 x 106 cell/ml x 1ml
EUR 1210
Description: tetracycline repressor in HEK293 stable cell line with Puromycin fusion marker

pCDH-CMV-HA-ABCB1 Plasmid

PVTB00597-4a 2 ug
EUR 356

pAAV- CMV- mCherry- U6- sgRNA

PVT11046 2 ug
EUR 301

pHG52d34.5-CMV-Mil-15-mCherry

PVT12170 2 ug
EUR 703

PHG52d34.5-CMV-Mil-21-mCherry

PVT12171 2 ug
EUR 703

pMXs-EF1-Puro Retroviral Vector

RTV-060 10 µg
EUR 624
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pLVX-EF1伪-IRES-puro

PVT2308 2 ug
EUR 325

pCDF1- MCS2- EF1- Puro Plasmid

PVT2338 2 ug
EUR 266

pBOB-EF1-FastFUCCI-Puro Plasmid

PVT16106 2 ug
EUR 325

pGreenFire1-STAT1 (plasmid)+ EF1-Puro

TR015PA-P 10 ug
EUR 786
  • Category: Lentiviral Technology

pGreenFire1-STAT (virus) + EF1-Puro

TR015VA-P >2 x 10^6 IFUs
EUR 786
  • Category: Lentiviral Technology

pGreenFire1-ISRE (plasmid)+ EF1-Puro

TR016PA-P 10 ug
EUR 786
  • Category: Lentiviral Technology

pGreenFire1-ISRE (virus) + EF1-Puro

TR016VA-P >2 x 10^6 IFUs
EUR 786
  • Category: Lentiviral Technology

pGreenFire1-Gal4 (plasmid)+ EF1-Puro

TR017PA-P 10 ug
EUR 786
  • Category: Lentiviral Technology

pGreenFire1-Gal4 (virus) + EF1-Puro

TR017VA-P >2 x 10^6 IFUs
EUR 786
  • Category: Lentiviral Technology

pGreenFire1-Nanog (plasmid)+ EF1-Puro

TR019PA-P 10 ug
EUR 786
  • Category: Lentiviral Technology

pGreenFire1-Nanog (virus) + EF1-Puro

TR019VA-P >2 x 10^6 IFUs
EUR 786
  • Category: Lentiviral Technology

pGreenFire1-Notch (plasmid)+ EF1-Puro

TR020PA-P 10 ug
EUR 786
  • Category: Lentiviral Technology

pGreenFire1-Notch (virus) + EF1-Puro

TR020VA-P >2 x 10^6 IFUs
EUR 786
  • Category: Lentiviral Technology

The active site of the protein. Of an overall assessment of the results obtained in this work, it is shown that the residues that the spectators are perceived as spectators play both an important role in the influence on the chromophore environment (volume of the cavity) and its dynamic (crossed correlations between them. Remote residues)

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