Effect of miR-146a and miR-155 on cardiac xenotransplantation.

Initial graft size and not the innate immune response limit survival of engrafted neural stem cells.

The purpose of this study was to investigate the expression level of miR-146a and miR-155 in cardiac xenograft models treated with immunosuppressant FK506, and to construct lentiviral vectors to further study the role of miR-146a and miR-155 in cardiac xenotransplantation. the expression levels of miR-146a and miR-155 is checked by quantitative polymerase chain reaction analysis and protein expression RELA, which is a member of the family of nuclear factor-kB, is checked by western blot analysis. Pre-miR-146a and miR-155 pre-designed and synthesized fragments according to MiRBase and cloned into plasmid pCDH1-MCS1-EF1-copGFP. recombinant plasmids were identified by enzyme digestion and sequencing.

Heart transplant survival time in FK506 treatment group increased significantly compared with the control group (P <0.05). In addition, the results of the histopathological grading was significantly decreased in the treatment group (P <0.05). A significant reduction in the level of protein expression Rela observed in the treatment group (P <0.05), along with a significant increase in the level of miR-146a expression (P <0.05) and a significant reduction in miR-155 expression levels ( P <0.05).

Digestion and sequencing findings suggest that miRNA insertion into plasmid pCDH1-MCS1-EF1-copGFP in accordance with pre-miRNAs, and concentrated into a lentiviral vector titer of 5 x 107 IFU / ml. These findings indicate that FK506 is able to inhibit the effect of rejection in cardiac xenotransplantation model of rat-to-rat. FK506 treatment alter the expression level of miR-146a and miR-155, which indicates that they may have an important role in regulating the immune response to the effects of rejection. miR-146a and miR-155 successfully constructed lentiviral vectors for further experiments both in vitro and in vivo.

Initial graft size and not the innate immune response limit survival of engrafted neural stem cells.
Initial graft size and not the innate immune response limit survival of engrafted neural stem cells.

Formulation Nonionic Surfactant vesicles (NISV) Prepared by Microfluidics for Therapeutic Delivery of siRNA into cancer cells.

Disturb small RNA (siRNA) has a vast potential as a therapeutic agent for reversible silencing of target genes anything interesting. The clinical application of siRNA requires the use of safe and effective delivery system. In this study, we investigated the use of non-ionic surfactant vesicles (NISV) for siRNA delivery. Various types of formulations NISV microfluidic synthesized by mixing and then evaluated for cytotoxicity and physiochemical properties.

NISV ability to carry and transfect siRNA targeting the green fluorescent protein (GFP) to the A549 that stably express GFP (copGFP-A549) were evaluated. Flow cytometry and Western blotting were used to study the expression of GFP knockdown, and significant knockdown was observed as a result of the delivery of siRNA into cells by NISV. This happens especially when using Tween 85, which is capable of reaching more than 70% GFP knockdown. NISV thus shown to provide a promising and effective platform for the delivery of siRNA therapies.The application of stem cells in the treatment of various degenerative diseases are very promising.

pSIH1-H1-siLuc-copGFP Packaged Positive Transduction Control

LV601B-1 >1 x 10^6 IFUs
EUR 838.8

pSIH1-H1-siLuc-copGFP positive transduction control, plasmid

LV601PB-1 10 ug
EUR 750

shRNA-H1 (Neg)-(blasticidin) lentivirus

H1(shRNA-Ctr)-Bsd 1 x107 IFU/ml x 200ul
EUR 418.8
Description: Pre-made optional inducible lentiviral shRNA expression particles under human H1 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a Blasticidin marker under Rsv promoter.

shRNA-H1 (Neg)-(Puromycin) lentivirus

H1(shRNA-Ctr)-Puro 1 x107 IFU/ml x 200ul
EUR 418.8
Description: Pre-made optional inducible lentiviral shRNA expression particles under human H1 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a Puromycin marker under Rsv promoter.

pCDF1-MCS2-EF1-copGFP cDNA Cloning and Expression Vector

CD111B-1 10 ug
EUR 762

pCDH-MCS-T2A-copGFP-MSCV Cloning and Expression Vector

CD523A-1 10 ug
EUR 783.6

PB-CMV-GreenPuro-H1-MCS shRNA cloning and expression vector

PBSI505A-1 10 ug
EUR 842.4

PB-EF1-GreenPuro-H1-MCS shRNA cloning and expression vector

PBSI506A-1 10 ug
EUR 842.4

shRNA-H1 (Neg)-( GFP-Bsd) lentivirus

H1(shRNA-Ctr)-GB 1 x107 IFU/ml x 200ul
EUR 418.8
Description: Pre-made optional inducible lentiviral shRNA expression particles under human H1 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a GFP-Blasticidin fusion marker under Rsv promoter.

shRNA-H1 (Neg)-( GFP-Puro) lentivirus

H1(shRNA-Ctr)-GP 1 x107 IFU/ml x 200ul
EUR 418.8
Description: Pre-made optional inducible lentiviral shRNA expression particles under human H1 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a GFP-Puromycin fusion marker under Rsv promoter.

shRNA-H1 (Neg)-( RFP-Bsd) lentivirus

H1(shRNA-Ctr)-RB 1 x107 IFU/ml x 200ul
EUR 418.8
Description: Pre-made optional inducible lentiviral shRNA expression particles under human H1 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a RFP-Blasticidin fusion marker under Rsv promoter.

shRNA-H1(Neg)-( RFP-Puro) lentivirus

H1(shRNA-Ctr)-RP 1 x107 IFU/ml x 200ul
EUR 418.8
Description: Pre-made optional inducible lentiviral shRNA expression particles under human H1 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a RFP-puromycin fusion marker under Rsv promoter.

pCDH-CMV-MCS-EF1-copGFP cDNA Cloning and Expression Vector

CD511B-1 10 ug
EUR 762

pCDH-CMV-MCS-t2A-copGFP cDNA Cloning and Expression Vector

CD524A-1 10 ug
EUR 783.6

shRNA-H1 (Neg)-( GFP-Bsd) lentivirus in PBS

H1(shRNA-Ctr)-GB-PBS 5 x107 IFU/ml x 200ul
EUR 852
Description: Pre-made optional inducible lentiviral shRNA expression particles under human H1 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a GFP-Blasticidin fusion marker under Rsv promoter. Virus was concentrated and provided in PBS solution.

shRNA-H1 (Neg)-( GFP-Puro) lentivirus in PBS

H1(shRNA-Ctr)-GP-PBS 5 x107 IFU/ml x 200ul
EUR 852
Description: Pre-made optional inducible lentiviral shRNA expression particles under human H1 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a GFP-Puromycin fusion marker under Rsv promoter. Virus was concentrated and provided in PBS solution.

shRNA-H1 (Neg)-( RFP-Bsd) lentivirus in PBS

H1(shRNA-Ctr)-RB-PBS 5 x107 IFU/ml x 200ul
EUR 852
Description: Pre-made optional inducible lentiviral shRNA expression particles under human H1 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a RFP-Blasticidin fusion marker under Rsv promoter. Virus was concentrated and provided in PBS solution.

shRNA-H1(Neg)-( RFP-Puro) lentivirus in PBS

H1(shRNA-Ctr)-RP-PBS 5 x107 IFU/ml x 200ul
EUR 852
Description: Pre-made optional inducible lentiviral shRNA expression particles under human H1 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a RFP-puromycin fusion marker under Rsv promoter. Virus was concentrated and provided in PBS solution.

pCDF1-MCS1 cDNA Cloning and Expression Vector

CD100A-1 10 ug
EUR 652.8

pAAVK-EF1? -MCS AAVanced Cloning and Expression Vector

AAV502A-1 10 ug
EUR 812.4

pCDH-CMV-MCS cDNA Cloning and Expression Vector

CD500B-1 10 ug
EUR 652.8

pCDH-CMV-MCS2 cDNA Cloning and Expression Vector

CD501A-1 10 ug
EUR 652.8

pCDH-EF1-MCS cDNA Cloning and Expression Vector

CD502A-1 10 ug
EUR 652.8

Constitutive (CAGs promoter) EEV cloning and expression vector

EEV600A-1 10 ug
EUR 812.4

Cumate-Inducible (CuO) EEV cloning and expression vector

EEV610A-1 10 ug
EUR 812.4

Human Histone H1 (H1) ELISA Kit

DLR-H1-Hu-48T 48T
EUR 620.4
Description: A sandwich quantitative ELISA assay kit for detection of Human Histone H1 (H1) in samples from tissue homogenates, cell lysates or other biological fluids.

Human Histone H1 (H1) ELISA Kit

DLR-H1-Hu-96T 96T
EUR 807.6
Description: A sandwich quantitative ELISA assay kit for detection of Human Histone H1 (H1) in samples from tissue homogenates, cell lysates or other biological fluids.

Human Histone H1 (H1) ELISA Kit

RDR-H1-Hu-48Tests 48 Tests
EUR 652.8

Human Histone H1 (H1) ELISA Kit

RDR-H1-Hu-96Tests 96 Tests
EUR 907.2

Human Histone H1 (H1) ELISA Kit

RD-H1-Hu-48Tests 48 Tests
EUR 625.2

Human Histone H1 (H1) ELISA Kit

RD-H1-Hu-96Tests 96 Tests
EUR 867.6

pCDH-EF1-MCS-(PGK-Puro) Cloning and Expression Vector

CD810A-1 10 ug
EUR 770.4

pCDH-EF1-MCS-(PGK-GFP) Cloning and Expression Vector

CD811A-1 10 ug
EUR 770.4

pSIF1-H1-siLuc-copGFP Positive Transduction Control (plasmid)

LV201PB-1 10 ug
EUR 750

pMC.CMV-GFP-H1-MCS (shRNA expression parental minicircle plasmid)

MNSI500A-1 10 ug
EUR 1016.4

PB-Cuo-shMCS-IRES-GFP-EF1-CymR-Puro Inducible shRNA Cloning and Expression Vector

PBQMSH812A-1 10 ug
EUR 1315.2

pAAVK-EF1?-MCS1-CMV-MCS2 AAVanced Cloning and Expression Vector

AAV503A-1 10 ug
EUR 812.4

pAAVK-EF1? -MCS-T2A-EGFP AAVanced Cloning and Expression Vector

AAV526A-1 10 ug
EUR 812.4

pAAVK-EF1? -MCS-T2A-Puro AAVanced Cloning and Expression Vector

AAV527A-1 10 ug
EUR 812.4

pAAVK-EF1? ?MCS-T2A-mRFP AAVanced Cloning and Expression Vector

AAV528A-1 10 ug
EUR 812.4

pAAVK-EF1?-MCS1-CMV-EGFP AAVanced Cloning and Expression Vector

AAV536A-1 10 ug
EUR 812.4

pAAVK-EF1?-MCS1-CMV-Puro AAVanced Cloning and Expression Vector

AAV537A-1 10 ug
EUR 812.4

pAAVK-EF1?-MCS1-CMV-mRFP AAVanced Cloning and Expression Vector

AAV538A-1 10 ug
EUR 812.4

pCDH-CMV-MCS-EF1-Puro cDNA Cloning and Expression Vector

CD510B-1 10 ug
EUR 762

pCDH-CMV-MCS-EF1-RFP cDNA Cloning and Expression Vector

CD512B-1 10 ug
EUR 762

pCDH-CMV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector

CD513B-1 10 ug
EUR 762

pCDH-CMV-MCS-EF1-Neo cDNA Cloning and Expression Vector

CD514B-1 10 ug
EUR 762

pCDH-CMV-MCS-EF1-Hygro cDNA Cloning and Expression Vector

CD515B-1 10 ug
EUR 762

pCDH-MCS-T2A-Puro-MSCV cDNA Cloning and Expression Vector

CD522A-1 10 ug
EUR 783.6

pCDH-EF1-MCS-IRES-GFP cDNA Cloning and Expression Vector

CD530A-2 10 ug
EUR 783.6

pCDH-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector

CD531A-2 10 ug
EUR 783.6

pCDH-EF1-MCS-IRES-Puro cDNA Cloning and Expression Vector

CD532A-2 10 ug
EUR 783.6

pCDH-EF1-MCS-IRES-Neo cDNA Cloning and Expression Vector

CD533A-2 10 ug
EUR 783.6

pCDH-UbC-MCS-EF1-Hygro cDNA Cloning and Expression Vector

CD615B-1 10 ug
EUR 762

pCDH-UbC-MCS-IRES-GFP cDNA Cloning and Expression Vector

CD630A-1 10 ug
EUR 783.6

pCDH-MSCV-MCS-EF1-Puro cDNA Cloning and Expression Vector

CD710B-1 10 ug
EUR 762

pCDH-MSCV-MCS-EF1-GFP cDNA Cloning and Expression Vector

CD711B-1 10 ug
EUR 762

pCDH-MSCV-MCS-IRES-GFP cDNA Cloning and Expression Vector

CD731B-1 10 ug
EUR 783.6

PB-CMV-MCS-EF1-Puro cDNA cloning and expression vector

PB510B-1 10 ug
EUR 842.4

PB-CMV-MCS-EF1-GFP cDNA cloning and expression vector

PB511B-1 10 ug
EUR 842.4

PB-CMV-MCS-EF1-RFP cDNA cloning and expression vector

PB512B-1 10 ug
EUR 842.4

PB-CMV-MCS-EF1-GreenPuro cDNA cloning and expression vector

PB513B-1 10 ug
EUR 842.4

PB-CMV-MCS-EF1-RedPuro cDNA Cloning and Expression Vector

PB514B-2 10 ug
EUR 842.4

PB-EF1-MCS-IRES-GFP cDNA cloning and expression vector

PB530A-2 10 ug
EUR 842.4

PB-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector

PB531A-2 10 ug
EUR 842.4

PB-EF1-MCS-IRES-Neo cDNA cloning and expression vector

PB533A-2 10 ug
EUR 842.4

PB-MSCV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector

PB713B-1 10 ug
EUR 842.4

pSIH1- ControlshRNA- Puro

PVT11395 2 ug
EUR 361.2

pCDF1-MCS2-EF1-Puro cDNA Cloning and Expression Lentivector

CD110B-1 10 ug
EUR 762

pCDH-CMV-MCS-EF1-RFP+Puro cDNA Cloning and Expression Vector

CD516B-2 10 ug
EUR 762

pCDH-MSCV-MCS-EF1-GFP+Puro cDNA Cloning and Expression Vector

CD713B-1 10 ug
EUR 762

pCDH-EF1-MCS-(PGK-GFP-T2A-Puro) Cloning and Expression Vector

CD813A-1 10 ug
EUR 770.4

pCDH-EF1-MCS-T2A-RFP (PGK-Puro) Cloning and Expression Vector

CD822A-1 10 ug
EUR 770.4

pCDH-EF1-MCS-T2A-GFP (PGK-Puro) Cloning and Expression Vector

CD823A-1 10 ug
EUR 770.4

HIV & FIV Positive Transduction Control Virus Sampler Kit [pSIF1- and pSIH-H1-siLuc-copGFP lentiviruses]

LV401A-1 >1 x 10^6 IFUs of each
EUR 838.8

pSIF1-H1-siLuc-copGFP Positive Transduction Control (pre-packaged virus)

LV201B-1 >1 x 10^6 IFUs
EUR 838.8

pMC.CMV-GFP-T2A-Puro-H1-MCS (shRNA expression parental minicircle plasmid)

MNSI505A-1 10 ug
EUR 1016.4

pMC.EF1-GFP-T2A-Puro-H1-MCS (shRNA expression parental minicircle plasmid)

MNSI506A-1 10 ug
EUR 1016.4

pCDH-EF1-MCS-T2A-Puro cDNA Cloning and Expression Vector 10 ug

CD527A-1 10 ug
EUR 783.6

pCDH-EF1-MCS-BGH-PGK-GFP-T2A-Puro cDNA Cloning and Expression Vector

CD550A-1 10 ug
EUR 750

XStamp cloning and expression lentivector MSCV-Leader-MCS-C1C2-EF1-Puro

XSTP710PA-1 10 ug
EUR 956.4

PB-Cuo-MCS-IRES-GFP-EF1-CymR-Puro Inducible cDNA Cloning and Expression Vector

PBQM812A-1 10 ug
EUR 1315.2

pGreenPuro shRNA expression lentivector (CMV)

SI505A-1 10 ug
EUR 787.2

pGreenPuro shRNA expression lentivector (EF1)

SI506A-1 10 ug
EUR 787.2

pRedPuro shRNA expression lentivector (EF1)

SI516A-1 10 ug
EUR 787.2

pCDH-EF1-Luc-T2A-copGFP

PVT17649 2 ug
EUR 508.8

pCDH- EF1- MCS- T2A- copGFP

PVT2336 2 ug
EUR 390

shRNA-U6 (Neg)-(blasticidin) lentivirus

U6(shRNA-Ctr)-Bsd 1 x107 IFU/ml x 200ul
EUR 418.8
Description: Pre-made lentiviral shRNA expression particles under human U6 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a Blasticidin marker under Rsv promoter.

shRNA-U6 (Neg)-(Puromycin) lentivirus

U6(shRNA-Ctr)-Puro 1 x107 IFU/ml x 200ul
EUR 418.8
Description: Pre-made lentiviral shRNA expression particles under human U6 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a Puromycin marker under Rsv promoter.

pYLEX1 - Expression Vector

FYY203-5MG 5mg Ask for price

Histone H1 (H1) Antibody

abx411412-02mg 0.2 mg
EUR 678

Recombinant Histone H1 (H1)

4-RPC527Hu01
  • EUR 442.56
  • EUR 242.40
  • EUR 1329.60
  • EUR 523.20
  • EUR 926.40
  • EUR 372.00
  • EUR 3144.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Histone H1 expressed in: E.coli

shRNA-U6 (Neg)-( GFP-Bsd) lentivirus

U6(shRNA-Ctr)-GB 1 x107 IFU/ml x 200ul
EUR 418.8
Description: Pre-made lentiviral shRNA expression particles under human U6 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a GFP-Blasticidin fusion marker under Rsv promoter.

shRNA-U6 (Neg)-( GFP-Puro) lentivirus

U6(shRNA-Ctr)-GP 1 x107 IFU/ml x 200ul
EUR 418.8
Description: Pre-made lentiviral shRNA expression particles under human U6 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a GFP-Puromycin fusion marker under Rsv promoter.

shRNA-U6 (Neg)-( RFP-Bsd) lentivirus

U6(shRNA-Ctr)-RB 1 x107 IFU/ml x 200ul
EUR 418.8
Description: Pre-made lentiviral shRNA expression particles under human U6 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a RFP-Blasticidin fusion marker under Rsv promoter.

shRNA-U6 (Neg)-( RFP-Puro) lentivirus

U6(shRNA-Ctr)-RP 1 x107 IFU/ml x 200ul
EUR 418.8
Description: Pre-made lentiviral shRNA expression particles under human U6 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a RFP-puromycin fusion marker under Rsv promoter.

pCDH-CMV-exosome booster-EF1-copGFP-Puro vector

PVT12109 2 ug
EUR 1149.6

pGreenPuro shRNA expression lentivector (CMV) [packaged virus]

SI505VB-1 >1 x 10^6 IFUs
EUR 859.2

pCDH- CMV- MCS- EF1- copGFP Plasmid

PVT2334 2 ug
EUR 390

pCDH- CMV- mCherry- EF1- CopGFP- Puro

PVT11078 2 ug
EUR 444

pCDH-CMV-GSTA1-EF1-copGFP Plasmid

PVT16093 2 ug
EUR 390

Histone H1 (Histone H1) Antibody

20-abx134258
  • EUR 427.20
  • EUR 644.40
  • EUR 260.40
  • 100 ul
  • 200 ul
  • 30 ul

Histone H1 (Histone H1) Antibody

20-abx009175
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

GEF-H1 (GEF-H1) Antibody

abx233412-100ug 100 ug
EUR 661.2

Histone H1 (Histone H1) Antibody

abx233881-100ug 100 ug
EUR 577.2

Rac1 Expression Vector Set

STA-454 1 kit
EUR 1765.2
Description: Rac1 Expression Vector Set contains 3 vectors: Rac wild type, T17N dominant negative mutant, and G12V constitutively active mutant.

Cdc42 Expression Vector Set

STA-455 1 kit
EUR 1765.2
Description: Cdc42 Expression Vector Set contains 3 vectors: Cdc42 wild type, T17N dominant negative mutant, and Q61L constitutively active mutant.

RhoA Expression Vector Set

STA-456 1 kit
EUR 1765.2
Description: RhoA Expression Vector Set contains 3 vectors: RhoA wild type, T19N dominant negative mutant, and G14V constitutively active mutant.

pAAV-MCS Expression Vector

VPK-410 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pscAAV-MCS Expression Vector

VPK-430 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pUC57-sgRNA expression vector

PVT18003 2 ug
EUR 309.6

Uni Seamless Cloning and Assembly Kit

20-abx098063
  • EUR 644.40
  • EUR 794.40
  • EUR 1245.60
  • 10 rxns
  • 20 rxns
  • 60 rxns

Human Histone H1 (H1) ELISA Kit

20-abx151821
  • EUR 8853.60
  • EUR 4719.60
  • EUR 1093.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Histone H1 (H1) CLIA Kit

20-abx493746
  • EUR 9567.60
  • EUR 5095.20
  • EUR 1177.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Histone H1 (H1) ELISA Kit

SEC527Hu-10x96wellstestplate 10x96-wells test plate
EUR 5677.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Histone H1 (H1) in tissue homogenates, cell lysates and other biological fluids.

Human Histone H1 (H1) ELISA Kit

SEC527Hu-1x48wellstestplate 1x48-wells test plate
EUR 572.76
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Histone H1 (H1) in tissue homogenates, cell lysates and other biological fluids.

Human Histone H1 (H1) ELISA Kit

SEC527Hu-1x96wellstestplate 1x96-wells test plate
EUR 766.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Histone H1 (H1) in tissue homogenates, cell lysates and other biological fluids.

Human Histone H1 (H1) ELISA Kit

SEC527Hu-5x96wellstestplate 5x96-wells test plate
EUR 3090.6
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Histone H1 (H1) in tissue homogenates, cell lysates and other biological fluids.

Human Histone H1 (H1) ELISA Kit

4-SEC527Hu
  • EUR 5738.40
  • EUR 3031.20
  • EUR 768.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Histone H1 (H1) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.

Human Histone H1 ELISA Kit (H1)

RK01531 96 Tests
EUR 625.2

Human Histone H1(H1)ELISA Kit

QY-E00049 96T
EUR 433.2

H1-7

H-1805.0005 5.0mg
EUR 254.4
Description: Sum Formula: C31H58N14O9; CAS# [65189-70-0] net

H1-7

H-1805.0025 25.0mg
EUR 907.2
Description: Sum Formula: C31H58N14O9; CAS# [65189-70-0] net

Cimicifugoside H1

TBW00230 unit Ask for price

Mammoside H1

TBZ0579 unit Ask for price

Merremoside H1

TBZ0592 unit Ask for price

pFUGW-H1

PVT17836 2 ug
EUR 360

H1 Protein

E35000 50 µg
EUR 174.84

pCDH- CMV- MCS- EF1- CopGFP- T2A- Puro

PVT2333 2 ug
EUR 390

pCDH- MSCV- MCS- EF1- CopGFP- T2A- Puro

PVT11079 2 ug
EUR 444

pCDH-CMV-Akaluc-EF1-CopGFP-T2A-Puro

PVT14490 2 ug
EUR 718.8

ELISA kit for Human H1 (Histone H1)

ELK5482 1 plate of 96 wells
EUR 518.4
Description: A sandwich ELISA kit for detection of Histone H1 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Luciferase (firefly) and CRE co-expression stable cell line (puromycin)

SC021-Puro 2 x 106 cell/ml x 1ml
EUR 1452
Description: Luciferase (firefly) and CRE recombinase co-expression stable cell line with Puromycin marker.

pCMV-GFP-LC3 Expression Vector

CBA-401 100 µL
EUR 1243.2
Description: Expression vector contains a fusion of GFP and LC3. A separate GFP control vector is also included.

GFP-Rac1 Expression Vector Set

STA-450 1 kit
EUR 1939.2
Description: GFP-Rac1 Expression Vector Set contains 3 vectors: Rac wild type, T17N dominant negative mutant, and Q61L constitutively active mutant. Each vector also contains a GFP reporter sequence.

GFP-Cdc42 Expression Vector Set

STA-451 1 kit
EUR 1939.2
Description: GFP-Cdc42 Expression Vector Set contains 3 vectors: Cdc42 wild type, T17N dominant negative mutant, and Q61L constitutively active mutant. Each vector also contains a GFP reporter sequence.

GFP-RhoA Expression Vector Set

STA-452 1 kit
EUR 1939.2
Description: GFP-RhoA Expression Vector Set contains 3 vectors: RhoA wild type, T19N dominant negative mutant, and Q63L constitutively active mutant. Each vector also contains a GFP reporter sequence.

H-Ras Expression Vector Set

STA-457 1 kit
EUR 1765.2
Description: H-Ras Expression Vector Set contains 3 vectors: H-Ras wild type, T19N dominant negative mutant, and G12V constitutively active mutant.

Active Rac1 Expression Vector Set

STA-458 1 kit
EUR 1765.2
Description: Active Rac1 Expression Vector Set contains 3 vectors expressing different constitutively active mutants of Rac1: Q61L, Q61L/F37A, and Q61L/Y40C.

pSMPUW-Puro Lentiviral Expression Vector

VPK-212 10 µg
EUR 748.8
Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer.

pSMPUW-Neo Lentiviral Expression Vector

VPK-213 10 µg
EUR 748.8
Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer.

pSMPUW-Hygro Lentiviral Expression Vector

VPK-214 10 µg
EUR 748.8
Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer.

pAAV-MCS Promoterless Expression Vector

VPK-411 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-IRES-Puro Expression Vector

VPK-415 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-IRES-Neo Expression Vector

VPK-416 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-IRES-Hygro Expression Vector

VPK-417 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-IRES-GFP Expression Vector

VPK-418 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-IRES-Bsd Expression Vector

VPK-419 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

Tet-on circRNA Expression Vector

PVT14643 2 ug
EUR 843.6

pCDH-CMV-efemp1-m-EF1-CopGFP-T2A-Puro

PVT17438 2 ug
EUR 360

pCDH-CMV-TXNL1-HA-EF1-CopGFP-T2A-Puro

PVT17693 2 ug
EUR 309.6

pCDH-CMV-CCDC68-HA-EF1-CopGFP-T2A-Puro

PVT17760 2 ug
EUR 309.6

pCDH-CMV-ccl3-m-EF1-CopGFP-T2A-Puro

PVT19054 2 ug
EUR 360

pCDH-CMV-PTX3-EF1-CopGFP-T2A-Puro Plasmid

PVT17116 2 ug
EUR 390

pCDH-CMV-MCS-EF1-copGFP-T2A-Puro plasmid

PVT17271 2 ug
EUR 568.8

Monoclonal Cytokeratin 8 (KRT8) Antibody - With BSA and Azide, Clone: H1

AMM00117G 0.05mg
EUR 475.2
Description: A Monoclonal antibody against Human Cytokeratin 8 (KRT8) - With BSA and Azide. The antibodies are raised in Mouse and are from clone H1. This antibody is applicable in WB and IHC, FC, IP, E

Monoclonal Cytokeratin 8 (KRT8) Antibody - Without BSA and Azide, Clone: H1

AMM00308G 0.1mg
EUR 580.8
Description: A Monoclonal antibody against Human Cytokeratin 8 (KRT8) - Without BSA and Azide. The antibodies are raised in Mouse and are from clone H1. This antibody is applicable in IHC-P, IF, FC

PSK-H1 Antibody

44396-100ul 100ul
EUR 302.4

PSK-H1 Antibody

44396-50ul 50ul
EUR 224.4

Histone H1 antibody

70R-49842 100 ul
EUR 292.8
Description: Purified Polyclonal Histone H1 antibody

nm23 H1 antibody

70R-50133 100 ul
EUR 292.8
Description: Purified Polyclonal nm23 H1 antibody

Histone H1 antibody

70R-33625 100 ug
EUR 392.4
Description: Rabbit polyclonal Histone H1 antibody

GEF H1 Antibody

49905-100ul 100ul
EUR 399.6

GEF H1 Antibody

49905-50ul 50ul
EUR 286.8

GEF-H1 Antibody

35429-100ul 100ul
EUR 468

GEF H1 Antibody

20-abx133112
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

nm23-H1 Antibody

20-abx008928
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

GEF H1 Antibody

20-abx008444
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

Histone H1 Antibody

ABF0876 100 ug
EUR 525.6

PSK- H1 Antibody

ABF9170 100 ug
EUR 525.6

PSK-H1 Antibody

20-abx218031
  • EUR 510.00
  • EUR 410.40
  • 100 ug
  • 50 ug

Histone H1 Antibody

20-abx325362
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

Histone H1 Antibody

20-abx326111
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

PSK-H1 Antibody

AF9170 200ul
EUR 420

anti-Nm23-H1

AR02-PA0003 100 ul
EUR 400.8
Description: Rabbit polyclonal to Nm23-H1

Histone H1 Antibody

1-CSB-PA000381
  • EUR 266.40
  • EUR 234.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against Histone H1. Recognizes Histone H1 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: WB;WB:1:1000-2000

GEF H1 Antibody

DF4437 200ul
EUR 420

GEF H1 Antibody

DF6996 200ul
EUR 420

RNase H1 Antibody

DF12084 200ul
EUR 420

GEF H1 Antibody

AF4783 200ul
EUR 540

Histone H1 Antibody

AF6572 100ul
EUR 420

Histone H1 Antibody

AF0876 200ul
EUR 420

Histone H1 Antibody

1-CSB-PA002919
  • EUR 266.40
  • EUR 234.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against Histone H1. Recognizes Histone H1 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: WB, IHC, IF, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/20000

anti-Histone H1

YF-PA22213 50 ul
EUR 435.6
Description: Mouse polyclonal to Histone H1

anti-Histone H1

YF-PA22214 50 ug
EUR 435.6
Description: Mouse polyclonal to Histone H1

anti-GEF H1

YF-PA25281 50 ul
EUR 400.8
Description: Mouse polyclonal to GEF H1

anti-Histone H1

YF-PA12238 50 ug
EUR 435.6
Description: Mouse polyclonal to Histone H1

anti-Histone H1

YF-PA12239 100 ug
EUR 483.6
Description: Rabbit polyclonal to Histone H1

anti-RNase H1

YF-PA26995 50 ul
EUR 400.8
Description: Mouse polyclonal to RNase H1

Histone H1 Antibody

V2565-100UG 100 ug
EUR 499
Description: Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.

However, the cell-based therapies can be limited by the problem of low survival of cells grafted and uncertainty about their fate. The combination of molecular imaging and contrast-enhanced MRI can provide more insight into the survival and behavior of transplanted stem cells. We explored the hair-follicle stem cell-derived bulge (HFBSCs) as a potential candidate for autologous cell-based therapies. HFBSCs were transduced with lentiviral construct to the gene coding for the bioluminescent (Luc2) and fluorescent (copGFP) reporter protein, and then loaded with magnetic nanoparticles to enable MRI visualization.

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