Effect of miR-146a and miR-155 on cardiac xenotransplantation.

Initial graft size and not the innate immune response limit survival of engrafted neural stem cells.

The purpose of this study was to investigate the expression level of miR-146a and miR-155 in cardiac xenograft models treated with immunosuppressant FK506, and to construct lentiviral vectors to further study the role of miR-146a and miR-155 in cardiac xenotransplantation. the expression levels of miR-146a and miR-155 is checked by quantitative polymerase chain reaction analysis and protein expression RELA, which is a member of the family of nuclear factor-kB, is checked by western blot analysis. Pre-miR-146a and miR-155 pre-designed and synthesized fragments according to MiRBase and cloned into plasmid pCDH1-MCS1-EF1-copGFP. recombinant plasmids were identified by enzyme digestion and sequencing.

Heart transplant survival time in FK506 treatment group increased significantly compared with the control group (P <0.05). In addition, the results of the histopathological grading was significantly decreased in the treatment group (P <0.05). A significant reduction in the level of protein expression Rela observed in the treatment group (P <0.05), along with a significant increase in the level of miR-146a expression (P <0.05) and a significant reduction in miR-155 expression levels ( P <0.05).

Digestion and sequencing findings suggest that miRNA insertion into plasmid pCDH1-MCS1-EF1-copGFP in accordance with pre-miRNAs, and concentrated into a lentiviral vector titer of 5 x 107 IFU / ml. These findings indicate that FK506 is able to inhibit the effect of rejection in cardiac xenotransplantation model of rat-to-rat. FK506 treatment alter the expression level of miR-146a and miR-155, which indicates that they may have an important role in regulating the immune response to the effects of rejection. miR-146a and miR-155 successfully constructed lentiviral vectors for further experiments both in vitro and in vivo.

Initial graft size and not the innate immune response limit survival of engrafted neural stem cells.
Initial graft size and not the innate immune response limit survival of engrafted neural stem cells.

Formulation Nonionic Surfactant vesicles (NISV) Prepared by Microfluidics for Therapeutic Delivery of siRNA into cancer cells.

Disturb small RNA (siRNA) has a vast potential as a therapeutic agent for reversible silencing of target genes anything interesting. The clinical application of siRNA requires the use of safe and effective delivery system. In this study, we investigated the use of non-ionic surfactant vesicles (NISV) for siRNA delivery. Various types of formulations NISV microfluidic synthesized by mixing and then evaluated for cytotoxicity and physiochemical properties.

NISV ability to carry and transfect siRNA targeting the green fluorescent protein (GFP) to the A549 that stably express GFP (copGFP-A549) were evaluated. Flow cytometry and Western blotting were used to study the expression of GFP knockdown, and significant knockdown was observed as a result of the delivery of siRNA into cells by NISV. This happens especially when using Tween 85, which is capable of reaching more than 70% GFP knockdown. NISV thus shown to provide a promising and effective platform for the delivery of siRNA therapies.The application of stem cells in the treatment of various degenerative diseases are very promising.

PB-EF1-GreenPuro-H1-MCS shRNA cloning and expression vector

PBSI506A-1 10 ug
EUR 638

pCDF1-MCS2-EF1-copGFP cDNA Cloning and Expression Vector

CD111B-1 10 ug
EUR 572

pCDH-MCS-T2A-copGFP-MSCV Cloning and Expression Vector

CD523A-1 10 ug
EUR 590

pCDH-CMV-MCS-EF1-copGFP cDNA Cloning and Expression Vector

CD511B-1 10 ug
EUR 572

pCDH-CMV-MCS-t2A-copGFP cDNA Cloning and Expression Vector

CD524A-1 10 ug
EUR 590

pCDF1-MCS1 cDNA Cloning and Expression Vector

CD100A-1 10 ug
EUR 483

PB-Cuo-shMCS-IRES-GFP-EF1-CymR-Puro Inducible shRNA Cloning and Expression Vector

PBQMSH812A-1 10 ug
EUR 1023

pGreen- Puro- pSIH1- H1- copGFP- T2A- Puro

PVT11116 2ug
EUR 260

pCDH-CMV-MCS cDNA Cloning and Expression Vector

CD500B-1 10 ug
EUR 483

pCDH-EF1-MCS cDNA Cloning and Expression Vector

CD502A-1 10 ug
EUR 483

pCDH-CMV-MCS2 cDNA Cloning and Expression Vector

CD501A-1 10 ug
EUR 483

pAAVK-EF1α -MCS AAVanced Cloning and Expression Vector

AAV502A-1 10 ug
EUR 614

Cumate-Inducible (CuO) EEV cloning and expression vector

EEV610A-1 10 ug
EUR 614

pCDH-EF1-MCS-(PGK-GFP) Cloning and Expression Vector

CD811A-1 10 ug
EUR 579

pCDH-EF1-MCS-(PGK-Puro) Cloning and Expression Vector

CD810A-1 10 ug
EUR 579

Constitutive (CAGs promoter) EEV cloning and expression vector

EEV600A-1 10 ug
EUR 614

PB-CMV-MCS-EF1-GFP cDNA cloning and expression vector

PB511B-1 10 ug
EUR 638

PB-CMV-MCS-EF1-RFP cDNA cloning and expression vector

PB512B-1 10 ug
EUR 638

PB-CMV-MCS-EF1-Puro cDNA cloning and expression vector

PB510B-1 10 ug
EUR 638

PB-EF1-MCS-IRES-GFP cDNA cloning and expression vector

PB530A-2 10 ug
EUR 638

PB-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector

PB531A-2 10 ug
EUR 638

PB-EF1-MCS-IRES-Neo cDNA cloning and expression vector

PB533A-2 10 ug
EUR 638

pCDH-CMV-MCS-EF1-RFP cDNA Cloning and Expression Vector

CD512B-1 10 ug
EUR 572

pCDH-CMV-MCS-EF1-Neo cDNA Cloning and Expression Vector

CD514B-1 10 ug
EUR 572

pCDH-CMV-MCS-EF1-Puro cDNA Cloning and Expression Vector

CD510B-1 10 ug
EUR 572

pCDH-EF1-MCS-IRES-GFP cDNA Cloning and Expression Vector

CD530A-2 10 ug
EUR 590

pCDH-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector

CD531A-2 10 ug
EUR 590

pCDH-EF1-MCS-IRES-Neo cDNA Cloning and Expression Vector

CD533A-2 10 ug
EUR 590

pCDH-UbC-MCS-IRES-GFP cDNA Cloning and Expression Vector

CD630A-1 10 ug
EUR 590

pCDH-MSCV-MCS-EF1-GFP cDNA Cloning and Expression Vector

CD711B-1 10 ug
EUR 572

pCDH-CMV-MCS-EF1-Hygro cDNA Cloning and Expression Vector

CD515B-1 10 ug
EUR 572

pCDH-MCS-T2A-Puro-MSCV cDNA Cloning and Expression Vector

CD522A-1 10 ug
EUR 590

pCDH-EF1-MCS-IRES-Puro cDNA Cloning and Expression Vector

CD532A-2 10 ug
EUR 590

pCDH-UbC-MCS-EF1-Hygro cDNA Cloning and Expression Vector

CD615B-1 10 ug
EUR 572

pCDH-MSCV-MCS-EF1-Puro cDNA Cloning and Expression Vector

CD710B-1 10 ug
EUR 572

pCDH-MSCV-MCS-IRES-GFP cDNA Cloning and Expression Vector

CD731B-1 10 ug
EUR 590

PB-CMV-MCS-EF1-RedPuro cDNA Cloning and Expression Vector

PB514B-2 10 ug
EUR 638

PB-CMV-MCS-EF1-GreenPuro cDNA cloning and expression vector

PB513B-1 10 ug
EUR 638

pAAVK-EF1α -MCS-T2A-EGFP AAVanced Cloning and Expression Vector

AAV526A-1 10 ug
EUR 614

pAAVK-EF1α -MCS-T2A-Puro AAVanced Cloning and Expression Vector

AAV527A-1 10 ug
EUR 614

pAAVK-EF1α –MCS-T2A-mRFP AAVanced Cloning and Expression Vector

AAV528A-1 10 ug
EUR 614

PB-MSCV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector

PB713B-1 10 ug
EUR 638

pCDH-CMV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector

CD513B-1 10 ug
EUR 572

pCDH-CMV-MCS-EF1-RFP+Puro cDNA Cloning and Expression Vector

CD516B-2 10 ug
EUR 572

pCDH-MSCV-MCS-EF1-GFP+Puro cDNA Cloning and Expression Vector

CD713B-1 10 ug
EUR 572

pCDH-EF1-MCS-(PGK-GFP-T2A-Puro) Cloning and Expression Vector

CD813A-1 10 ug
EUR 579

pCDH-EF1-MCS-T2A-RFP (PGK-Puro) Cloning and Expression Vector

CD822A-1 10 ug
EUR 579

pCDH-EF1-MCS-T2A-GFP (PGK-Puro) Cloning and Expression Vector

CD823A-1 10 ug
EUR 579

pAAVK-EF1α-MCS1-CMV-MCS2 AAVanced Cloning and Expression Vector

AAV503A-1 10 ug
EUR 614

pAAVK-EF1α-MCS1-CMV-EGFP AAVanced Cloning and Expression Vector

AAV536A-1 10 ug
EUR 614

pAAVK-EF1α-MCS1-CMV-Puro AAVanced Cloning and Expression Vector

AAV537A-1 10 ug
EUR 614

pAAVK-EF1α-MCS1-CMV-mRFP AAVanced Cloning and Expression Vector

AAV538A-1 10 ug
EUR 614

pCDH-EF1-MCS-T2A-Puro cDNA Cloning and Expression Vector 10 µg

CD527A-1 10 ug
EUR 590

pCDH-EF1-MCS-BGH-PGK-GFP-T2A-Puro cDNA Cloning and Expression Vector

CD550A-1 10 ug
EUR 563

PB-CuO-CMV-MCS-EF1α-CymR-Puro Inducible cDNA Cloning and Expression Vector

PBQM800A-1 10 µg
EUR 921

PB-Cuo-MCS-IRES-GFP-EF1-CymR-Puro Inducible cDNA Cloning and Expression Vector

PBQM812A-1 10 ug
EUR 1023

ProFold-C1. Baculovirus chaperone vector for expression of cytoplasmic and nuclear proteins.

A2 25 ul
EUR 830
Description: Protein expression

ProFold-C2. Baculovirus chaperone vector for expression of cytoplasmic and nuclear proteins.

A3 25 ul
EUR 830
Description: Protein expression

ProFold-ER1. Baculovirus chaperone vector for expression of secreted and membrane proteins.

A4 25 ul
EUR 830
Description: Protein expression

ER1 Kit. Baculovirus chaperone vectors for expression of secreted and membrane proteins.

K23 1 Kit
EUR 995
Description: Protein expression

ER1-bee Kit. Baculovirus chaperone vectors for expression of secreted and membrane proteins.

K24 1 Kit
EUR 995
Description: Protein expression

pAcAB3. Baculovirus plasmid vector for expression of up to 3 proteins.

B2 50 ul
EUR 420
Description: Protein expression

pCDF1-MCS2-EF1-Puro cDNA Cloning and Expression Lentivector

CD110B-1 10 ug
EUR 572

ProFold-PDI. Baculovirus chaperone vector for expression of cysteine-rich proteins.

A7 25 ul
EUR 830
Description: Protein expression

XStamp cloning and expression lentivector MSCV-Leader-MCS-C1C2-EF1-Puro

XSTP710PA-1 10 ug
EUR 730

baculoCOMPLETE protein expression kit

400100 5 reactions
EUR 874.2

GCP (green control plasmid for GFP expression)

C15 50 ul
EUR 145
Description: Protein expression

baculoCOMPLETE protein expression kit + baculoQUANT all-in-one

400101 5 + 100 reactions
EUR 1419.8

pSIH1-H1-copGFP-T2A-Puro

PVTY00070 2ug
EUR 280

pLenti-SFFV-MCS-EF1-copGFP Lentiviral Expression Vector

LV460 10 µg
EUR 416.5

CNGA2 Expression vector

60642 20 µg
EUR 900
Description: The CNGA2 expression vector is designed to express human cyclic nucleotide-gated cation channel 2 (CNGA2) in mammalian cells.

CNGA2 Expression vector

GWB-PSFA61 20ug Ask for price

pYLEX1 - Expression Vector

FYY203-5MG 5mg Ask for price

pMC.CMV-GFP-H1-MCS (shRNA expression parental minicircle plasmid)

MNSI500A-1 10 ug
EUR 779

Rac1 Expression Vector Set

STA-454 1 kit
EUR 1765.2
Description: Rac1 Expression Vector Set contains 3 vectors: Rac wild type, T17N dominant negative mutant, and G12V constitutively active mutant.

RhoA Expression Vector Set

STA-456 1 kit
EUR 1765.2
Description: RhoA Expression Vector Set contains 3 vectors: RhoA wild type, T19N dominant negative mutant, and G14V constitutively active mutant.

Cdc42 Expression Vector Set

STA-455 1 kit
EUR 1765.2
Description: Cdc42 Expression Vector Set contains 3 vectors: Cdc42 wild type, T17N dominant negative mutant, and Q61L constitutively active mutant.

pAAV-MCS Expression Vector

VPK-410 10 µg
EUR 530

pAAV-MCS Expression Vector

MBS169528-001mg 0.01mg
EUR 695

pAAV-MCS Expression Vector

MBS169528-5x001mg 5x0.01mg
EUR 2955

pscAAV-MCS Expression Vector

VPK-430 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pUC57-sgRNA expression vector

PVT18003 2ug
EUR 162

H-Ras Expression Vector Set

STA-457 1 kit
EUR 1765.2
Description: H-Ras Expression Vector Set contains 3 vectors: H-Ras wild type, T19N dominant negative mutant, and G12V constitutively active mutant.

ANPRA/Aequorin Expression vector

60641 20 µg
EUR 900
Description: The ANPRA/Aequorin expression vector is designed to co-express human atrial natriuretic peptide receptor A (ANPRA, also called natriuretic peptide receptor A/guanylate cyclase A) and jellyfish (Aequorea victoria) Aequorin in mammalian cells.

ANPRA/Aequorin Expression vector

GWB-PS0F6A 20ug Ask for price

GFP-Rac1 Expression Vector Set

STA-450 1 kit
EUR 1939.2
Description: GFP-Rac1 Expression Vector Set contains 3 vectors: Rac wild type, T17N dominant negative mutant, and Q61L constitutively active mutant. Each vector also contains a GFP reporter sequence.

GFP-RhoA Expression Vector Set

STA-452 1 kit
EUR 1939.2
Description: GFP-RhoA Expression Vector Set contains 3 vectors: RhoA wild type, T19N dominant negative mutant, and Q63L constitutively active mutant. Each vector also contains a GFP reporter sequence.

GFP-Cdc42 Expression Vector Set

STA-451 1 kit
EUR 1939.2
Description: GFP-Cdc42 Expression Vector Set contains 3 vectors: Cdc42 wild type, T17N dominant negative mutant, and Q61L constitutively active mutant. Each vector also contains a GFP reporter sequence.

Active Rac1 Expression Vector Set

STA-458 1 kit
EUR 1765.2
Description: Active Rac1 Expression Vector Set contains 3 vectors expressing different constitutively active mutants of Rac1: Q61L, Q61L/F37A, and Q61L/Y40C.

pSIH1-H1-CoGFP

PVTY01169 2ug
EUR 280

pCMV-GFP-LC3 Expression Vector

CBA-401 100 µL
EUR 1243.2
Description: Expression vector contains a fusion of GFP and LC3. A separate GFP control vector is also included.

Lenti-III-HA Expression Vector

LV022 10 μg
EUR 575

Tet-on circRNA Expression Vector

PVT14643 2ug
EUR 580

pAAV-IRES-Neo Expression Vector

VPK-416 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-IRES-GFP Expression Vector

VPK-418 10 µg
EUR 530

pAAV-IRES-Bsd Expression Vector

VPK-419 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

Lenti-III-UbC Expression Vector

G300 10 μg
EUR 575

Lenti-III-PGK Expression Vector

G305 10 μg
EUR 575

pAAV-IRES-GFP Expression Vector

MBS169533-001mg 0.01mg
EUR 695

pAAV-IRES-GFP Expression Vector

MBS169533-5x001mg 5x0.01mg
EUR 2955

pAAV-IRES-Puro Expression Vector

VPK-415 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-IRES-Hygro Expression Vector

VPK-417 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pMC.CMV-GFP-T2A-Puro-H1-MCS (shRNA expression parental minicircle plasmid)

MNSI505A-1 10 ug
EUR 779

pMC.EF1-GFP-T2A-Puro-H1-MCS (shRNA expression parental minicircle plasmid)

MNSI506A-1 10 ug
EUR 779

Active H-Ras Expression Vector Set

STA-459 1 kit
EUR 1765.2
Description: Active H-Ras Expression Vector Set contains 3 vectors expressing different constitutively active mutants of H-Ras: V12, V12S35, and V12C40.

pSIH1-H1-siLuc-copGFP Packaged Positive Transduction Control

LV601B-1 >1 x 10^6 IFUs
EUR 634

pSIH1-H1-siLuc-copGFP positive transduction control, plasmid

LV601PB-1 10 ug
EUR 563

pSMPUW-Neo Lentiviral Expression Vector

VPK-213 10 µg
EUR 505

pAAV-MCS Promoterless Expression Vector

VPK-411 10 µg
EUR 530

pLenti-MCS Lentiviral Expression Vector

LV000 10 µg
EUR 416.5

pSMPUW-Neo Lentiviral Expression Vector

MBS169522-001mg 0.01mg
EUR 665

pSMPUW-Neo Lentiviral Expression Vector

MBS169522-5x001mg 5x0.01mg
EUR 2825

pAAV-MCS Promoterless Expression Vector

MBS169529-001mg 0.01mg
EUR 695

pAAV-MCS Promoterless Expression Vector

MBS169529-5x001mg 5x0.01mg
EUR 2955

pRedPuro shRNA expression lentivector (EF1)

SI516A-1 10 ug
EUR 594

pSMPUW-Puro Lentiviral Expression Vector

VPK-212 10 µg
EUR 505

pSMPUW-Puro Lentiviral Expression Vector

MBS169521-001mg 0.01mg
EUR 665

pSMPUW-Puro Lentiviral Expression Vector

MBS169521-5x001mg 5x0.01mg
EUR 2825

pSMPUW-Hygro Lentiviral Expression Vector

VPK-214 10 µg
EUR 505

pSMPUW-Hygro Lentiviral Expression Vector

MBS169523-001mg 0.01mg
EUR 665

pSMPUW-Hygro Lentiviral Expression Vector

MBS169523-5x001mg 5x0.01mg
EUR 2825

pGreenPuro shRNA expression lentivector (CMV)

SI505A-1 10 ug
EUR 594

pGreenPuro shRNA expression lentivector (EF1)

SI506A-1 10 ug
EUR 594

Baculovirus Expression Vector pAc-l-Fc

MBS641019-0005mg 0.005mg
EUR 825

Baculovirus Expression Vector pAc-l-Fc

MBS641019-5x0005mg 5x0.005mg
EUR 3560

Baculovirus Expression Vector pAc-k-Fc

MBS641024-0005mg 0.005mg
EUR 825

Baculovirus Expression Vector pAc-k-Fc

MBS641024-5x0005mg 5x0.005mg
EUR 3560

Baculovirus expression vector pAc-g-CH3

30R-AB001 5 ug
EUR 685
Description: Baculovirus cassette vector pAc-l-CH3 for the expression of human, humanized or chimeric IgG(lambda) in insect cells and secretion of assembled antibodies into the supernatant.

Baculovirus expression vector pAc-g-CH3

GWB-EC6621 0.01 ml Ask for price

Baculovirus Expression Vector pAc-k-CH3

MBS641022-0005mg 0.005mg
EUR 825

Baculovirus Expression Vector pAc-k-CH3

MBS641022-5x0005mg 5x0.005mg
EUR 3560

Baculovirus Expression Vector pAc-l-CH3

MBS641026-0005mg 0.005mg
EUR 820

Baculovirus Expression Vector pAc-l-CH3

MBS641026-5x0005mg 5x0.005mg
EUR 3530

Baculovirus expression vector pAc-g-CH3

MBS537173-0005mg 0.005mg
EUR 865

Baculovirus expression vector pAc-g-CH3

MBS537173-5x0005mg 5x0.005mg
EUR 3745

Exoenzyme C3 (Rho Inhibitor) Expression Vector

STA-460 1 kit
EUR 860.4
Description: This mammalian expression vector codes for Exoenzyme C3, a Rho inhibitor.

pSMPUW-GFP-LC3 Lentiviral Expression Vector

LTV-801 10 µg
EUR 1444.8
Description: Expression vector contains a fusion of GFP and LC3. A separate GFP control vector is also included.

pSMPUW-IRES-Neo Lentiviral Expression Vector

VPK-216 10 µg
EUR 505

pSMPUW-IRES-Neo Lentiviral Expression Vector

MBS169525-001mg 0.01mg
EUR 665

pSMPUW-IRES-Neo Lentiviral Expression Vector

MBS169525-5x001mg 5x0.01mg
EUR 2825

pSMPUW-IRES-Puro Lentiviral Expression Vector

VPK-215 10 µg
EUR 505

pSMPUW-IRES-Puro Lentiviral Expression Vector

MBS169524-001mg 0.01mg
EUR 665

pSMPUW-IRES-Puro Lentiviral Expression Vector

MBS169524-5x001mg 5x0.01mg
EUR 2825

pSMPUW-IRES-Hygro Lentiviral Expression Vector

VPK-217 10 µg
EUR 505

pSMPUW-IRES-Hygro Lentiviral Expression Vector

MBS169526-001mg 0.01mg
EUR 665

pSMPUW-IRES-Hygro Lentiviral Expression Vector

MBS169526-5x001mg 5x0.01mg
EUR 2825

Mouse IL-23 Enhanced Episomal Expression Vector

EEV651A-1 100 ug
EUR 812

FoldHelper-57P. Recombinant baculovirus expressing ERp57 and PDI.

H17 1 ml
EUR 295
Description: Protein expression

pMXs-miRNA-GFP/Puro Retroviral Expression Vector

RTV-017 10 ?g
EUR 505

pMXs-miRNA-GFP/Puro Retroviral Expression Vector

MBS169492-001mg 0.01mg
EUR 665

pMXs-miRNA-GFP/Puro Retroviral Expression Vector

MBS169492-5x001mg 5x0.01mg
EUR 2825

pLenti-MCS-PGK-PURO Lentiviral Expression Vector

LV001 10 µg
EUR 416.5

pSMPUW-IRES-Blasticidin Lentiviral Expression Vector

VPK-219 10 µg
EUR 505

pSMPUW-IRES-Blasticidin Lentiviral Expression Vector

MBS169527-001mg 0.01mg
EUR 665

pSMPUW-IRES-Blasticidin Lentiviral Expression Vector

MBS169527-5x001mg 5x0.01mg
EUR 2825

pSMPUW Universal Lentiviral Expression Vector (Promoterless)

VPK-211 10 µg
EUR 505

pSMPUW Universal Lentiviral Expression Vector (Promoterless)

MBS169520-001mg 0.01mg
EUR 665

pSMPUW Universal Lentiviral Expression Vector (Promoterless)

MBS169520-5x001mg 5x0.01mg
EUR 2825

pLenti-EF1-MCS-PGK-Neo Lentiviral Expression Vector

LV202 10 µg
EUR 416.5

pLenti-EF1-MCS-PGK-RFP Lentiviral Expression Vector

LV220 10 µg
EUR 416.5

pLenti-CAG-MCS-PGK-NEO Lentiviral Expression Vector

LV302 10 µg
EUR 416.5

pLenti-CAG-MCS- EF1-coGFP Lentiviral Expression Vector

LV360 10 µg
EUR 416.5

Excision only piggyBac Transposase expression vector, 50 rxns

PB220PA-1 10 ug
EUR 471

pLenti-CMV-MCS-EF1α-RFP Lentiviral Expression Vector

LV120 10 µg
EUR 416.5

pLenti-EF1-MCS-PGK-PURO Lentiviral Expression Vector

LV201 10 µg
EUR 416.5

pLenti-CAG-MCS-PGK-HygR Lentiviral Expression Vector

LV303 10 µg
EUR 416.5

pLenti-CAG-MCS-EF1-PURO Lentiviral Expression Vector

LV351 10 µg
EUR 416.5

pLenti-SFFV-MCS-PGK-NEO Lentiviral Expression Vector

LV402 10 µg
EUR 416.5

pGreenPuro shRNA expression lentivector (CMV) [packaged virus]

SI505VB-1 >1 x 10^6 IFUs
EUR 652

pLenti-EF1-MCS-PGK-Hygro Lentiviral Expression Vector

LV203 10 µg
EUR 416.5

pLenti-SFFV-MCS-PGK-Puro Lentiviral Expression Vector

LV401 10 µg
EUR 416.5

pLenti-SFFV-MCS-EF1-Puro Lentiviral Expression Vector

LV451 10 µg
EUR 416.5

pLenti-SFFV-MCS-PGK-Hygro Lentiviral Expression Vector

LV403 10 µg
EUR 416.5

pLenti-CAG-MCS-PGK-TdTomato Lentiviral Expression Vector

LV320 10 µg
EUR 416.5

pLenti-CAG-MCS-EF1-TdTomato Lentiviral Expression Vector

LV370 10 µg
EUR 416.5

pLenti-SFFV-MCS-PGK-TdTomato Lentiviral Expression Vector

LV420 10 µg
EUR 416.5

pLenti-SFFV-MCS-EF1-TdTomato Lentiviral Expression Vector

LV470 10 µg
EUR 416.5

pLenti-MCS-EF1-GFP-T2A-puro Lentiviral Expression Vector

LV011 10 µg
EUR 416.5

pLenti-MCS-EF1-RFP-T2A-puro Lentiviral Expression Vector

LV021 10 µg
EUR 416.5

GFP-tagged ORF clone of Mus musculus caveolin, caveolae protein 1 (Cav1) in a GFP expression vector

MG201562 10 µg Ask for price

Super piggyBac Transposase expression vector (replaces PB200A-1), 50 rxns

PB210PA-1 10 ug
EUR 471

pLenti-EF1-MCS-PGK-GFP-T2A-PURO Lentiviral Expression Vector

LV211 10 µg
EUR 416.5

pLenti-EF1-MCS-PGK-GFP-T2A-Neo Lentiviral Expression Vector

LV212 10 µg
EUR 416.5

pLenti-EF1-MCS-PGK-RFP-T2A-PURO Lentiviral Expression Vector

LV221 10 µg
EUR 416.5

pLenti-CMV-MCS-EF1α-GFP-T2A-PURO Lentiviral Expression Vector

LV111 10 µg
EUR 416.5

pLenti-CMV-MCS-EF1α-RFP-T2A-PURO Lentiviral Expression Vector

LV121 10 µg
EUR 416.5

pLenti-EF1-MCS-PGK-GFP-T2A-Hygro Lentiviral Expression Vector

LV213 10 µg
EUR 416.5

pLenti-CAG-MCS-PGK-coGFP-T2A-NEO Lentiviral Expression Vector

LV312 10 µg
EUR 416.5

pLenti-CAG-MCS-PGK-coGFP-T2A-HygR Lentiviral Expression Vector

LV313 10 µg
EUR 416.5

pLenti-CAG-MCS-EF1-coGFP-T2A-PURO Lentiviral Expression Vector

LV361 10 µg
EUR 416.5

However, the cell-based therapies can be limited by the problem of low survival of cells grafted and uncertainty about their fate. The combination of molecular imaging and contrast-enhanced MRI can provide more insight into the survival and behavior of transplanted stem cells. We explored the hair-follicle stem cell-derived bulge (HFBSCs) as a potential candidate for autologous cell-based therapies. HFBSCs were transduced with lentiviral construct to the gene coding for the bioluminescent (Luc2) and fluorescent (copGFP) reporter protein, and then loaded with magnetic nanoparticles to enable MRI visualization.

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