Neuronal differentiation of hair-follicle-bulge-derived stem cells co-cultured with mouse cochlear modiolus explants.

Neuronal differentiation of hair-follicle-bulge-derived stem cells co-cultured with mouse cochlear modiolus explants.

Stem cell-based repair auditory neurons may represent an attractive therapeutic option to restore sensorineural hearing loss. stem cells of hair-follicle-bulge-derived (HFBSCs) are promising candidates for this type of therapy, because they (1) have the nature of migration, allowing migration after transplantation, (2) can differentiate into sensory neurons and glial cells, and (3) can easily be harvested in relatively high amounts. However, HFBSCs never used for this purpose.

We hypothesize that HFBSCs can be used to repair the auditory nerve cell-based and we have researched their migration and incorporation into the cochlear modiolus explant and subsequent differentiation of them. Modiolus explants derived from adult wild type mice were cultured in the presence of HFBSCs EF1α-copGFP-transduced, constitutively expressing green fluorescent protein copepods (copGFP). Also, explants modiolus without hair cells were co-cultured with DCX-copGFP HFBSCs-transduced, indicating copGFP on doublecortin expression during neuronal differentiation.

HFBSC speed migration towards modiolus explants is calculated, and after two weeks, co-cultures were fixed and processed for immunohistochemical staining. EF1α-copGFP HFBSC rapid migration speed: 80.5 ± 6.1 m / h. After arriving in explants, cells form a pattern fasciculus and change their phenotype into neural cell types Atoh1-positive. DCX-copGFP HFBSCs green-fluorescent after integration into the explants, justify the differentiation of neurons from cells. These results indicate that HFBSC derived progenitor neuronal migration and can integrate into the cochlear modiolus explants, while adapting their phenotypes depending on the micro environment. Thus, HFBSCs demonstrate the potential to be employed in cell-based therapies to repair the auditory nerve.

The immunomodulatory and self-renewable human features adipose-derived mesenchymal stem cells (HAD-MSCs) mark their importance in regenerative medicine. Interleukin (IL) -23 as proinflammatory cytokines Hitting the regulatory T cells and promote the response of T helper 17 and T helper 1 cells. This initiation pathways of inflammation and immunosuppression in several autoimmune diseases.

 Neuronal differentiation of hair-follicle-bulge-derived stem cells co-cultured with mouse cochlear modiolus explants.
Neuronal differentiation of hair-follicle-bulge-derived stem cells co-cultured with mouse cochlear modiolus explants.

[Construction of eukaryotic expression vector fibroblast activation protein and the formation of stable over-expression in squamous cell carcinoma of the mouth].

OBJECTIVE
This study aimed to construct vectors fibroblast activation protein (FAP) over-expression lentivinus to investigate transfection in cell lines and build a stable FAP SCC9 over-expression of oral squamous cell lines.


METHOD
The cDNA of the FAP gene from squamous cell carcinoma of the oral tissues (OSCC) was amplified by polymerase chain reaction (PCR) and subkloning into eukaryotic expression vector pCDH-CMV-MCS-EF1-copGFP. Recombinant plasmids were lined up and then transfected into cell lines SCC9. Further, cell lines which over-expressed FAP SCC9 stable founded by fluorescence activated cell sorting (FACS). Expression of green fluorescent protein (GFP) was detected with a fluorescence microscope, and over-expression of FAP identified by real-time PCR and Western blot.


RESULTS
FAP gene amplified by PCR and then cloned into a vector, whose sequence is identical to that in GenBank. GFP was expressed in transfected cells. Furthermore, FAP over-expression in transfected cells was detected by real-time PCR and Western blot.

pCDH-CMV-MCS-t2A-copGFP cDNA Cloning and Expression Vector

CD524A-1 10 ug
EUR 653.00
  • Category: Lentiviral Technology

pCDH-MCS-T2A-Puro-MSCV cDNA Cloning and Expression Vector

CD522A-1 10 ug
EUR 653.00
  • Category: Lentiviral Technology

pCDH- EF1- MCS- T2A- copGFP

PVT2336 2 ug
EUR 325.00

pCDH-CMV-MCS-EF1-copGFP cDNA Cloning and Expression Vector

CD511B-1 10 ug
EUR 635.00
  • Category: Lentiviral Technology

pCDH-MSCV-MCS-EF1-Puro cDNA Cloning and Expression Vector

CD710B-1 10 ug
EUR 635.00
  • Category: Lentiviral Technology

pCDH-MSCV-MCS-IRES-GFP cDNA Cloning and Expression Vector

CD731B-1 10 ug
EUR 653.00
  • Category: Lentiviral Technology

pCDH-MSCV-MCS-EF1-GFP cDNA Cloning and Expression Vector

CD711B-1 10 ug
EUR 635.00
  • Category: Lentiviral Technology

pCDH-MSCV-MCS-EF1-GFP+Puro cDNA Cloning and Expression Vector

CD713B-1 10 ug
EUR 635.00
  • Category: Lentiviral Technology

pCDH- CMV- MCS- EF1- CopGFP- T2A- Puro

PVT2333 2 ug
EUR 325.00

pCDH-EF1-MCS-T2A-GFP (PGK-Puro) Cloning and Expression Vector

CD823A-1 10 ug
EUR 642.00
  • Category: Lentiviral Technology

pCDH-EF1-MCS-(PGK-GFP-T2A-Puro) Cloning and Expression Vector

CD813A-1 10 ug
EUR 642.00
  • Category: Lentiviral Technology

pCDH-EF1-MCS-T2A-RFP (PGK-Puro) Cloning and Expression Vector

CD822A-1 10 ug
EUR 642.00
  • Category: Lentiviral Technology

pCDH-CMV-MCS-EF1-copGFP-T2A-Puro plasmid

PVT17271 2 ug
EUR 474.00

pCDH-EF1-MCS-T2A-Puro cDNA Cloning and Expression Vector 10 ug

CD527A-1 10 ug
EUR 653.00
  • Category: Lentiviral Technology

pCDH-CMV-MCS cDNA Cloning and Expression Vector

CD500B-1 10 ug
EUR 544.00
  • Category: Lentiviral Technology

pCDH-EF1-MCS cDNA Cloning and Expression Vector

CD502A-1 10 ug
EUR 544.00
  • Category: Lentiviral Technology

pCDH-EF1-MCS-BGH-PGK-GFP-T2A-Puro cDNA Cloning and Expression Vector

CD550A-1 10 ug
EUR 625.00
  • Category: Lentiviral Technology

pCDH-EF1-MCS-(PGK-GFP) Cloning and Expression Vector

CD811A-1 10 ug
EUR 642.00
  • Category: Lentiviral Technology

pCDH-EF1-MCS-(PGK-Puro) Cloning and Expression Vector

CD810A-1 10 ug
EUR 642.00
  • Category: Lentiviral Technology

pCDH-EF1-Luc-T2A-copGFP

PVT17649 2 ug
EUR 424.00

PB-MSCV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector

PB713B-1 10 ug
EUR 702.00
  • Category: PiggyBac Technology

pCDH-CMV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector

CD513B-1 10 ug
EUR 635.00
  • Category: Lentiviral Technology

pCDH-CMV-MCS-EF1-Hygro cDNA Cloning and Expression Vector

CD515B-1 10 ug
EUR 635.00
  • Category: Lentiviral Technology

pCDH-EF1-MCS-IRES-GFP cDNA Cloning and Expression Vector

CD530A-2 10 ug
EUR 653.00
  • Category: Lentiviral Technology

pCDH-EF1-MCS-IRES-Puro cDNA Cloning and Expression Vector

CD532A-2 10 ug
EUR 653.00
  • Category: Lentiviral Technology

pCDH-UbC-MCS-IRES-GFP cDNA Cloning and Expression Vector

CD630A-1 10 ug
EUR 653.00
  • Category: Lentiviral Technology

pCDH-CMV-MCS-EF1-Puro cDNA Cloning and Expression Vector

CD510B-1 10 ug
EUR 635.00
  • Category: Lentiviral Technology

pCDH-CMV-MCS-EF1-RFP cDNA Cloning and Expression Vector

CD512B-1 10 ug
EUR 635.00
  • Category: Lentiviral Technology

pCDH-CMV-MCS-EF1-Neo cDNA Cloning and Expression Vector

CD514B-1 10 ug
EUR 635.00
  • Category: Lentiviral Technology

pCDH-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector

CD531A-2 10 ug
EUR 653.00
  • Category: Lentiviral Technology

pCDH-EF1-MCS-IRES-Neo cDNA Cloning and Expression Vector

CD533A-2 10 ug
EUR 653.00
  • Category: Lentiviral Technology

pCDH-UbC-MCS-EF1-Hygro cDNA Cloning and Expression Vector

CD615B-1 10 ug
EUR 635.00
  • Category: Lentiviral Technology

pCDH-EF1-MCS*-T2A-GFP

CD526A-1 10 ug
EUR 653.00
  • Category: Lentiviral Technology

pCDH- CMV- MCS- EF1- copGFP Plasmid

PVT2334 2 ug
EUR 325.00

pAAVK-EF1? -MCS-T2A-EGFP AAVanced Cloning and Expression Vector

AAV526A-1 10 ug
EUR 677.00
  • Category: Vectors

pAAVK-EF1? -MCS-T2A-Puro AAVanced Cloning and Expression Vector

AAV527A-1 10 ug
EUR 677.00
  • Category: Vectors

pAAVK-EF1? ?MCS-T2A-mRFP AAVanced Cloning and Expression Vector

AAV528A-1 10 ug
EUR 677.00
  • Category: Vectors

pCDH-CMV-MCS-EF1-RFP+Puro cDNA Cloning and Expression Vector

CD516B-2 10 ug
EUR 635.00
  • Category: Lentiviral Technology

XStamp cloning and expression lentivector MSCV-Leader-MCS-C1C2-EF1-Puro

XSTP710PA-1 10 ug
EUR 797.00
  • Category: Exosome

pCDH- EF1- MCS- T2A- Puro Plasmid

PVT2335 2 ug
EUR 325.00

pCDH-CMV-Akaluc-EF1-CopGFP-T2A-Puro

PVT14490 2 ug
EUR 599.00

pCDH-CuO-MCS-T2A-GFP co-inducible T2A lentivector

QM521A-1 10 ug
EUR 679.00
  • Category: Lentiviral Technology

pCDH-CuO-MCS-T2A-RFP co-inducible T2A lentivector

QM522A-1 10 ug
EUR 679.00
  • Category: Lentiviral Technology

pSIH1-H1-copGFP shRNA Cloning and Expression Vector

SI501A-1 10 ug
EUR 567.00
  • Category: Lentiviral Technology

pCDH-EF1-MCS-T2A-Puro (CD520A) plasmid

PVT17273 2 ug
EUR 474.00

pCDH- CMV- MCS- EF1- turboRFP- T2A- Puro

PVT11077 2 ug
EUR 370.00

pCDH-Cuo-MCS-EF1-CymR-T2A-Puro

QM800A-1 10 ug
EUR 1310.00
  • Category: Lentiviral Technology

pCDH-CMV-PTX3-EF1-CopGFP-T2A-Puro Plasmid

PVT17116 2 ug
EUR 325.00

pCDH-CMV-efemp1-m-EF1-CopGFP-T2A-Puro

PVT17438 2 ug
EUR 300.00

pCDH-CMV-TXNL1-HA-EF1-CopGFP-T2A-Puro

PVT17693 2 ug
EUR 258.00

pCDH-CMV-CCDC68-HA-EF1-CopGFP-T2A-Puro

PVT17760 2 ug
EUR 258.00

pCDH-CMV-ccl3-m-EF1-CopGFP-T2A-Puro

PVT19054 2 ug
EUR 300.00

pCDH-CMV-MCS2 cDNA Cloning and Expression Vector

CD501A-1 10 ug
EUR 544.00
  • Category: Lentiviral Technology

pAAVK-EF1? -MCS AAVanced Cloning and Expression Vector

AAV502A-1 10 ug
EUR 677.00
  • Category: Vectors

pCDF1-MCS2-EF1-copGFP cDNA Cloning and Expression Vector

CD111B-1 10 ug
EUR 635.00
  • Category: Lentiviral Technology

pCDH-CuO-MCS empty cloning lentivector

QM500A-1 10 ug
EUR 597.00
  • Category: Lentiviral Technology

BLIV 2.0 Reporter: MSCV-Luciferase-EF1a-copGFP-T2A-Puro Lentivector Plasmid

BLIV713PA-1 10 ug
EUR 803.00
  • Category: Bioluminescent Imaging

pCDH-CMV-MCS-EF1-copGFP+Puro cDNA Clon/Exp Vector [pre-packaged]

CD513VB-1 >1 x 10^6 IFUs
EUR 786.00
  • Category: Lentiviral Technology

pCDH-CMV-MCS-EF1-copGFP cDNA Clon/Exp Vector [pre-packaged virus]

CD511VB-1 >1 x 10^6 IFUs
EUR 786.00
  • Category: Lentiviral Technology

Scramble control hairpin in pCDH-CMV-MCS-EF1-copGFP (CD511B-1)

PMIRH000PA-1 Bacterial Streak
EUR 656.00
  • Category: MicroRNA Tools

PB-CMV-GreenPuro-H1-MCS shRNA cloning and expression vector

PBSI505A-1 10 ug
EUR 702.00
  • Category: PiggyBac Technology

PB-EF1-GreenPuro-H1-MCS shRNA cloning and expression vector

PBSI506A-1 10 ug
EUR 702.00
  • Category: PiggyBac Technology

PB-CMV-MCS-EF1-Puro cDNA cloning and expression vector

PB510B-1 10 ug
EUR 702.00
  • Category: PiggyBac Technology

PB-CMV-MCS-EF1-GFP cDNA cloning and expression vector

PB511B-1 10 ug
EUR 702.00
  • Category: PiggyBac Technology

PB-CMV-MCS-EF1-RFP cDNA cloning and expression vector

PB512B-1 10 ug
EUR 702.00
  • Category: PiggyBac Technology

PB-CMV-MCS-EF1-GreenPuro cDNA cloning and expression vector

PB513B-1 10 ug
EUR 702.00
  • Category: PiggyBac Technology

PB-CMV-MCS-EF1-RedPuro cDNA Cloning and Expression Vector

PB514B-2 10 ug
EUR 702.00
  • Category: PiggyBac Technology

PB-EF1-MCS-IRES-GFP cDNA cloning and expression vector

PB530A-2 10 ug
EUR 702.00
  • Category: PiggyBac Technology

PB-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector

PB531A-2 10 ug
EUR 702.00
  • Category: PiggyBac Technology

PB-EF1-MCS-IRES-Neo cDNA cloning and expression vector

PB533A-2 10 ug
EUR 702.00
  • Category: PiggyBac Technology

XPack MSCV-XP-MCS-EF1-Puro Cloning Lentivector

XPAK710PA-1 10 ug
EUR 797.00
  • Category: Exosome

BLIV 2.0 Reporter: MSCV-Luciferase-EF1a-copGFP-T2A-Puro Pre-packaged Virus

BLIV713VA-1 >2 x10^6 IFUs
EUR 803.00
  • Category: Bioluminescent Imaging

pAAV-MCS Expression Vector

VPK-410 10 µg
EUR 647.00
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pscAAV-MCS Expression Vector

VPK-430 10 µg
EUR 647.00
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pCDH-CMV-GSTA1-EF1-copGFP Plasmid

PVT16093 2 ug
EUR 325.00

pCDH- CMV- mCherry- EF1- CopGFP- Puro

PVT11078 2 ug
EUR 370.00

T2A-GFP Co-Expression HR Targeting Vector [T2A-GFP-pA-loxP-EF1?-RFP-T2A-Puro-pA-LoxP-MCS]

HR130PA-1 10 ug
EUR 1023.00
  • Category: HR Donors

pCDH- CMV- MCS- EF1- puro

PVT11075 2 ug
EUR 370.00

pCDH- CMV- MCS- EF1- Hygro

PVT11076 2 ug
EUR 370.00

pCDH-CMV-MCS-EF1-copGFP cDNA Clon/Exp Vector [pre-packaged Ultra-High Titer virus]

CD511VB-UHT >1x10^9 IFUs
EUR 1041.00
  • Category: Lentiviral Technology

pCDH-EF1-Luc2-T2A-ZsGreen1

PVT17917 2 ug
EUR 424.00

pCDH-CMV-exosome booster-EF1-copGFP-Puro vector

PVT12109 2 ug
EUR 958.00

pAAV-MCS Promoterless Expression Vector

VPK-411 10 µg
EUR 647.00
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pMC.CMV-GFP-T2A-Puro-H1-MCS (shRNA expression parental minicircle plasmid)

MNSI505A-1 10 ug
EUR 847.00
  • Category: Minicircle Technology

pMC.EF1-GFP-T2A-Puro-H1-MCS (shRNA expression parental minicircle plasmid)

MNSI506A-1 10 ug
EUR 847.00
  • Category: Minicircle Technology

GFP-T2A-Luciferase Co-Expression HR Targeting Vector [GFP-T2A-Luc-pA-loxP-EF1?-RFP-T2A-Puro-pA-LoxP-MCS]

HR150PA-1 10 ug
EUR 1023.00
  • Category: HR Donors

pCDH-CMV-MCS-EF1a-GFP+BSD

PVT17282 2 ug
EUR 391.00

pCDH-CMV-MCS-EF1-EGFP-IRFP

PVT17586 2 ug
EUR 424.00

pCDH- ECMV- MCS- 3*Flag- EF1

PVT11080 2 ug
EUR 370.00

PB-Cuo-MCS-IRES-GFP-EF1-CymR-Puro Inducible cDNA Cloning and Expression Vector

PBQM812A-1 10 ug
EUR 1096.00
  • Category: PiggyBac Technology

pCDH-NFAT-Luc2-T2A-tdTomato Plasmid

PVT16080 2 ug
EUR 325.00

pCDH- EF1- luc2- T2A- Tdtomato Plasmid

PVT2337 2 ug
EUR 241.00

pCDH-EF1-CymR-T2A-Puro (plasmid)

QM200PA-2 10 ug
EUR 597.00
  • Category: Lentiviral Technology

pCDH-EF1-CymR-T2A-Puro (virus)

QM200VA-2 >1 x 10^6 IFUs
EUR 636.00
  • Category: Lentiviral Technology

pCDH-EF1-CymR-T2A-RFP (plasmid)

QM300PA-1 10 ug
EUR 597.00
  • Category: Lentiviral Technology

pCDH-EF1-CymR-T2A-RFP (virus)

QM300VA-1 >1 x 10^6 IFUs
EUR 636.00
  • Category: Lentiviral Technology

pCDH-EF1-CymR-T2A-Neo (plasmid)

QM400PA-2 10 ug
EUR 581.00
  • Category: Lentiviral Technology

pCDH-EF1-CymR-T2A-Neo (virus)

QM400VA-2 >1 x 10^6 IFUs
EUR 620.00
  • Category: Lentiviral Technology

pGreen- Puro- pSIH1- H1- copGFP- T2A- Puro

PVT11116 2 ug
EUR 370.00

MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid

CASLV120PA-1 10 ug
EUR 697.00
  • Category: Cas9

pCDH-CMV-MCS-EF1-Puro (CD510B) plasmid

PVT17272 2 ug
EUR 474.00

pCDH-CuO-MCS-IRES-GFP-EF1-CymR-T2A-Puro All-in-one inducible IRES lentivector

QM812B-1 10 ug
EUR 1310.00
  • Category: Lentiviral Technology

pCDF1-MCS1 cDNA Cloning and Expression Vector

CD100A-1 10 ug
EUR 544.00
  • Category: Lentiviral Technology

pCDH-ME-APOE-Luc2-T2A-tdTomato Plasmid

PVT17253 2 ug
EUR 370.00

pCDH-CMV-GLUT1-EF1-turboRFP-T2A-Puro

PVT13588 2 ug
EUR 599.00

pCDH-CuO-MCS-EF1-GFP+Puro dual promoter and dual marker lentivector

QM513B-1 10 ug
EUR 679.00
  • Category: Lentiviral Technology

pCDH-CuO-MCS-EF1-RFP+Puro dual promoter and dual marker lentivector

QM516B-2 10 ug
EUR 679.00
  • Category: Lentiviral Technology

pCDH-CMV-MCS-EF1-Puro-FLAG-HIF2 alpha

PVT12166 2 ug
EUR 370.00

pCDH-CuO-MCS-EF1-GFP dual promoter lentivector

QM511B-1 10 ug
EUR 679.00
  • Category: Lentiviral Technology

pCDH-CuO-MCS-EF1-RFP dual promoter lentivector

QM512B-1 10 ug
EUR 679.00
  • Category: Lentiviral Technology

Constitutive (CAGs promoter) EEV cloning and expression vector

EEV600A-1 10 ug
EUR 677.00
  • Category: Episomal Vectors

Cumate-Inducible (CuO) EEV cloning and expression vector

EEV610A-1 10 ug
EUR 677.00
  • Category: Episomal Vectors

pSIH1-H1-Puro shRNA Cloning and Expression Vector

SI500A-1 10 ug
EUR 567.00
  • Category: Lentiviral Technology

pSIH1-H1-H2Kk shRNA Cloning and Expression Vector

SI502A-1 10 ug
EUR 567.00
  • Category: Lentiviral Technology

pCDH-Cuo-RFP-T2A-GFP (positive control plasmid)

QM350PA-1 10 ug
EUR 597.00
  • Category: Lentiviral Technology

pCDH-Cuo-RFP-T2A-GFP (positive control virus)

QM350VA-1 >1 x 10^6 IFUs
EUR 636.00
  • Category: Lentiviral Technology

pCDH-CuO-MCS-IRES-GFP co-inducible IRES lentivector

QM530A-2 10 ug
EUR 679.00
  • Category: Lentiviral Technology

pCDH-CuO-MCS-IRES-RFP co-inducible IRES lentivector

QM531A-2 10 ug
EUR 679.00
  • Category: Lentiviral Technology

XPack MSCV-XP-GFP-EF1-Puro Expression Lentivector

XPAK730PA-1 10 ug
EUR 826.00
  • Category: Exosome

XPack MSCV-XP-GFP-EF1-Puro Expression Lentivirus

XPAK730VA-1 >1 x 10^6 IFUs
EUR 826.00
  • Category: Exosome

XPack MSCV-XP-Luciferase-EF1-Puro Expression Lentivector

XPAK732PA-1 10 ug
EUR 826.00
  • Category: Exosome

XPack MSCV-XP-Luciferase-EF1-Puro Expression Lentivirus

XPAK732VA-1 >1 x 10^6 IFUs
EUR 826.00
  • Category: Exosome

pCDH-EF1a-Piranha-T2A-Puro virus (>1x10^6 IFUs)

PTPD527VA-1 >1x10^6 IFUs
EUR 697.00

pCDH-CMV-MCS-EF1-aRFP cDNA Clon/Exp Vector [pre-packaged]

CD512VA-1 >1 x 10^6 IFUs
EUR 786.00
  • Category: Lentiviral Technology

MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV120PA-KIT 1 Kit
EUR 1132.00
  • Category: Cas9

Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid

CASLV220PA-1 10 ug
EUR 697.00
  • Category: Cas9

Minicircle Dual Reporter: MSCV-RFP-T2A-Luciferase DNA (30 ug)

BLIV506MC-1 30 ug
EUR 716.00
  • Category: Bioluminescent Imaging

Minicircle Dual Reporter: MSCV-GFP-T2A-Luciferase DNA (30 ug)

BLIV507MC-1 30 ug
EUR 716.00
  • Category: Bioluminescent Imaging

pFC-EF1-MCS-pA-PGK-RFP-T2A-Puro PhiC31 Donor Vector

FC550A-1 10ug
EUR 773.00
  • Category: PhiC31 Integrase Tools

pFC-PGK-MCS-pA-EF1-GFP-T2A-Puro PhiC31 Donor Vector

FC551A-1 10ug
EUR 773.00
  • Category: PhiC31 Integrase Tools

pAAVK-EF1?-MCS1-CMV-MCS2 AAVanced Cloning and Expression Vector

AAV503A-1 10 ug
EUR 677.00
  • Category: Vectors

pAAVK-EF1?-MCS1-CMV-EGFP AAVanced Cloning and Expression Vector

AAV536A-1 10 ug
EUR 677.00
  • Category: Vectors

pAAVK-EF1?-MCS1-CMV-Puro AAVanced Cloning and Expression Vector

AAV537A-1 10 ug
EUR 677.00
  • Category: Vectors

pAAVK-EF1?-MCS1-CMV-mRFP AAVanced Cloning and Expression Vector

AAV538A-1 10 ug
EUR 677.00
  • Category: Vectors

pCDH-CMV-MCS-EF1-RFP+Puro cDNA Clon/Exp Vector [pre-packaged]

CD516VB-2 >1 x 10^6 IFUs
EUR 772.00
  • Category: Lentiviral Technology

Minicircle Dual Reporter: MSCV-RFP-T2A-Luciferase Parental Plasmid (10 ug)

BLIV506MN-1 10 ug
EUR 972.00
  • Category: Bioluminescent Imaging

Minicircle Dual Reporter: MSCV-GFP-T2A-Luciferase Parental Plasmid (10 ug)

BLIV507MN-1 10 ug
EUR 972.00
  • Category: Bioluminescent Imaging

pCDF1-MCS2-EF1-Puro cDNA Cloning and Expression Lentivector

CD110B-1 10 ug
EUR 635.00
  • Category: Lentiviral Technology

pCDH-CMV-MCS-EF1-Puro cDNA Vector cDNA Clon/Exp Vector [pre-packaged virus]

CD510VB-1 >1 x 10^6 IFUs
EUR 786.00
  • Category: Lentiviral Technology

PCDH

EF007042 96 Tests
EUR 689.00

PCDH

EF007043 96 Tests
EUR 689.00

pCDH-CMV-Piranha-EF1-GFP-T2A-Puro virus (>1x10^6 IFUs)

PTPD513VA-1 >1x10^6 IFUs
EUR 697.00

MSCV-hspCas9-T2A-Puro SmartNuclease Pre-Packaged Lentiviral Particles [>10^6 IFUs]

CASLV120VA-1 2 x 25ul
EUR 697.00
  • Category: Cas9

pMC.CMV-GFP-H1-MCS (shRNA expression parental minicircle plasmid)

MNSI500A-1 10 ug
EUR 847.00
  • Category: Minicircle Technology

Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV220PA-KIT 1 Kit
EUR 1132.00
  • Category: Cas9

MSCV-hspCas9-T2A-Puro-H1-gRNA linearized all-in-one SmartNuclease Lentivector Plasmid

CASLV320PA-1 10 rxn
EUR 805.00
  • Category: Cas9

MSCV-Mouse Foxp3-EF1-GFP-T2A-Puro Stable Jurkat cell line (Puro resistant)

TCL110C-1 5 x 10^6 cells/ml
EUR 1682.00
  • Category: T Cell Products

MSCV-Human Foxp3-EF1-GFP-T2A-Puro Stable Jurkat cell line (Puro resistant)

TCL210C-1 5 x 10^6 cells/ml
EUR 1682.00
  • Category: T Cell Products

MSCV-Mouse RORgt-EF1-GFP-T2A-Puro Stable Jurkat cell line (Puro resistant)

TCL310C-1 5 x 10^6 cells/ml
EUR 1682.00
  • Category: T Cell Products

MSCV-Human RORgt-EF1-GFP-T2A-Puro Stable Jurkat cell line (Puro resistant)

TCL410C-1 5 x 10^6 cells/ml
EUR 1682.00
  • Category: T Cell Products

MSCV JMJD3 Plasmid

PVT17098 2 ug
EUR 325.00

XPack CMV-XP-MCS-EF1-Puro Cloning Lentivector

XPAK510PA-1 10 ug
EUR 797.00
  • Category: Exosome

GFP-Fusion HR Targeting Vector [GFP-pA-LoxP-EF1?-RFP-T2A-Puro-pA-LoxP-MCS]

HR120PA-1 10 ug
EUR 1023.00
  • Category: HR Donors

GFP-Fusion HR Targeting Vector [GFP-pA-LoxP-EF1a-RFP-T2A-Hygro-pA-LoxP-MCS]

HR220PA-1 10 ug
EUR 1023.00
  • Category: HR Donors

PB-Cuo-shMCS-IRES-GFP-EF1-CymR-Puro Inducible shRNA Cloning and Expression Vector

PBQMSH812A-1 10 ug
EUR 1096.00
  • Category: PiggyBac Technology

pGEN- MCS

PVT10565 2 ug
EUR 370.00

pLEX- MCS

PVT11092 2 ug
EUR 301.00

Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Pre-Packaged Lentiviral Particles [>10^6 IFUs]

CASLV220VA-1 2 x 25ul
EUR 697.00
  • Category: Cas9

PinPoint-HR attP Placement Vector ? Empty MCS for cloning HR Arms

PIN400A-1 10ug
EUR 1268.00
  • Category: PinPoint Integrase Tools

MSCV-hspCas9-H1-gRNA SmartNuclease vector

CAS960A-1 10 ug
EUR 805.00
  • Category: Cas9

pCDH- CMV- IDH1

PVT10169 2 ug
EUR 301.00

pCDH- CMV- CAMK2G

PVT10240 2 ug
EUR 301.00

pCDH- CMV- Rnd2

PVT10402 2 ug
EUR 301.00

Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro-H1-gRNA linearized all-in-one SmartNuclease Lentivector Plasmid

CASLV420PA-1 10 rxn
EUR 805.00
  • Category: Cas9

MCS ORF Vector (Human) (pORF)

ORF023399 1.0 ug DNA Ask for price

pMC.CMV-MCS-SV40polyA

MN501A-1 10 ug
EUR 735.00
  • Category: Minicircle Technology

pMC.EF1-MCS-SV40polyA

MN502A-1 10 ug
EUR 735.00
  • Category: Minicircle Technology

pCDNA3.1-MCS-6

PVT15973 2 ug
EUR 325.00

pEnCMV-MCS-3

PVT17410 2 ug
EUR 300.00

pMyosin-MCS-PSmOrange

PVT17668 2 ug
EUR 383.00

pECMV-MCS-Myc

PVT17857 2 ug
EUR 341.00

pLEGFP-MCS-FLAG

PVT18164 2 ug
EUR 341.00

pAAV- MCS Plasmid

PVT2102 2 ug
EUR 241.00

pT7- His- MCS

PVT10595 2 ug
EUR 266.00

pEU- E01- MCS

PVT11379 2 ug
EUR 370.00

pAAVS1-P-MCS

PVT12296 2 ug
EUR 703.00

pMD-AOX-MCS

PVT14289 2 ug
EUR 911.00

pPS-MSCV-GFP-RFP (plasmid)

LV605PA-1 10 ug
EUR 656.00
  • Category: Lentiviral Technology

PCDH-X / Y Antibody

20-abx218014
  • EUR 425.00
  • EUR 342.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

PCDH-X/Y Antibody

DF8867 200ul
EUR 304.00
Description: PCDH-X/Y Antibody detects endogenous levels of total PCDH-X/Y.

PCDH- X/Y Antibody

ABD8867 100 ug
EUR 438.00

PCDH-X/Y Antibody

45549-100ul 100ul
EUR 252.00

PCDH-X/Y Antibody

45549-50ul 50ul
EUR 187.00

pCDH- puro- Bcl- XL

PVT10290 2 ug
EUR 301.00

pCDH-513B-CTH Plasmid

PVTB00700-4c 2 ug
EUR 356.00

pCDH-puro-CSF1R Plasmid

PVTB00726-4a 2 ug
EUR 356.00

pYLEX1 - Expression Vector

FYY203-5MG 5mg Ask for price

pORB-MCS-sIRES-VSVG

ABP-BM-sIRESVG 5 ug Ask for price
    • Product line: Sapphire Baculo3Mam
    • Brand:

pCS2-EGFP-MCS-3

PVT17867 2 ug
EUR 300.00

pCDNA3.1- Myc- BioID2- MCS

PVT10689 2 ug
EUR 301.00


CONCLUSION
Recombinant eukaryotic expression vector pCDH-FAP successfully constructed. These results provide a basis for further study on the function of FAP in vitro.

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