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Neuronal differentiation of hair-follicle-bulge-derived stem cells co-cultured with mouse cochlear modiolus explants.
Stem cell-based repair auditory neurons may represent an attractive therapeutic option to restore sensorineural hearing loss. stem cells of hair-follicle-bulge-derived (HFBSCs) are promising candidates for this type of therapy, because they (1) have the nature of migration, allowing migration after transplantation, (2) can differentiate into sensory neurons and glial cells, and (3) can easily be harvested in relatively high amounts. However, HFBSCs never used for this purpose.
We hypothesize that HFBSCs can be used to repair the auditory nerve cell-based and we have researched their migration and incorporation into the cochlear modiolus explant and subsequent differentiation of them. Modiolus explants derived from adult wild type mice were cultured in the presence of HFBSCs EF1α-copGFP-transduced, constitutively expressing green fluorescent protein copepods (copGFP). Also, explants modiolus without hair cells were co-cultured with DCX-copGFP HFBSCs-transduced, indicating copGFP on doublecortin expression during neuronal differentiation.
HFBSC speed migration towards modiolus explants is calculated, and after two weeks, co-cultures were fixed and processed for immunohistochemical staining. EF1α-copGFP HFBSC rapid migration speed: 80.5 ± 6.1 m / h. After arriving in explants, cells form a pattern fasciculus and change their phenotype into neural cell types Atoh1-positive. DCX-copGFP HFBSCs green-fluorescent after integration into the explants, justify the differentiation of neurons from cells. These results indicate that HFBSC derived progenitor neuronal migration and can integrate into the cochlear modiolus explants, while adapting their phenotypes depending on the micro environment. Thus, HFBSCs demonstrate the potential to be employed in cell-based therapies to repair the auditory nerve.
The immunomodulatory and self-renewable human features adipose-derived mesenchymal stem cells (HAD-MSCs) mark their importance in regenerative medicine. Interleukin (IL) -23 as proinflammatory cytokines Hitting the regulatory T cells and promote the response of T helper 17 and T helper 1 cells. This initiation pathways of inflammation and immunosuppression in several autoimmune diseases.
Neuronal differentiation of hair-follicle-bulge-derived stem cells co-cultured with mouse cochlear modiolus explants.
[Construction of eukaryotic expression vector fibroblast activation protein and the formation of stable over-expression in squamous cell carcinoma of the mouth].
OBJECTIVE This study aimed to construct vectors fibroblast activation protein (FAP) over-expression lentivinus to investigate transfection in cell lines and build a stable FAP SCC9 over-expression of oral squamous cell lines.
METHOD The cDNA of the FAP gene from squamous cell carcinoma of the oral tissues (OSCC) was amplified by polymerase chain reaction (PCR) and subkloning into eukaryotic expression vector pCDH-CMV-MCS-EF1-copGFP. Recombinant plasmids were lined up and then transfected into cell lines SCC9. Further, cell lines which over-expressed FAP SCC9 stable founded by fluorescence activated cell sorting (FACS). Expression of green fluorescent protein (GFP) was detected with a fluorescence microscope, and over-expression of FAP identified by real-time PCR and Western blot.
RESULTS FAP gene amplified by PCR and then cloned into a vector, whose sequence is identical to that in GenBank. GFP was expressed in transfected cells. Furthermore, FAP over-expression in transfected cells was detected by real-time PCR and Western blot.
pCDH-CMV-MCS-t2A-copGFP cDNA Cloning and Expression Vector
CD524A-1
SBI
10 ug
EUR 653
Category: Lentiviral Technology
pCDH-MCS-T2A-Puro-MSCV cDNA Cloning and Expression Vector
CD522A-1
SBI
10 ug
EUR 653
Category: Lentiviral Technology
pCDH-CMV-MCS-EF1-copGFP cDNA Cloning and Expression Vector
CD511B-1
SBI
10 ug
EUR 635
Category: Lentiviral Technology
pCDH-MSCV-MCS-EF1-Puro cDNA Cloning and Expression Vector
CD710B-1
SBI
10 ug
EUR 635
Category: Lentiviral Technology
pCDH-MSCV-MCS-EF1-GFP cDNA Cloning and Expression Vector
CD711B-1
SBI
10 ug
EUR 635
Category: Lentiviral Technology
pCDH-MSCV-MCS-IRES-GFP cDNA Cloning and Expression Vector
CD731B-1
SBI
10 ug
EUR 653
Category: Lentiviral Technology
pCDH-MSCV-MCS-EF1-GFP+Puro cDNA Cloning and Expression Vector
CD713B-1
SBI
10 ug
EUR 635
Category: Lentiviral Technology
pCDH-EF1-MCS-(PGK-GFP-T2A-Puro) Cloning and Expression Vector
CD813A-1
SBI
10 ug
EUR 642
Category: Lentiviral Technology
pCDH-EF1-MCS-T2A-RFP (PGK-Puro) Cloning and Expression Vector
CD822A-1
SBI
10 ug
EUR 642
Category: Lentiviral Technology
pCDH-EF1-MCS-T2A-GFP (PGK-Puro) Cloning and Expression Vector
CD823A-1
SBI
10 ug
EUR 642
Category: Lentiviral Technology
pCDH-EF1-MCS-T2A-Puro cDNA Cloning and Expression Vector 10 ug
CD527A-1
SBI
10 ug
EUR 653
Category: Lentiviral Technology
pCDH-CMV-MCS cDNA Cloning and Expression Vector
CD500B-1
SBI
10 ug
EUR 544
Category: Lentiviral Technology
pCDH-EF1-MCS cDNA Cloning and Expression Vector
CD502A-1
SBI
10 ug
EUR 544
Category: Lentiviral Technology
pCDH-EF1-MCS-BGH-PGK-GFP-T2A-Puro cDNA Cloning and Expression Vector
CD550A-1
SBI
10 ug
EUR 625
Category: Lentiviral Technology
pCDH-EF1-MCS-(PGK-Puro) Cloning and Expression Vector
CD810A-1
SBI
10 ug
EUR 642
Category: Lentiviral Technology
pCDH-EF1-MCS-(PGK-GFP) Cloning and Expression Vector
CD811A-1
SBI
10 ug
EUR 642
Category: Lentiviral Technology
PB-MSCV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector
PB713B-1
SBI
10 ug
EUR 702
Category: PiggyBac Technology
pCDH-EF1-MCS-IRES-GFP cDNA Cloning and Expression Vector
CD530A-2
SBI
10 ug
EUR 653
Category: Lentiviral Technology
pCDH-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector
CD531A-2
SBI
10 ug
EUR 653
Category: Lentiviral Technology
pCDH-EF1-MCS-IRES-Puro cDNA Cloning and Expression Vector
CD532A-2
SBI
10 ug
EUR 653
Category: Lentiviral Technology
pCDH-EF1-MCS-IRES-Neo cDNA Cloning and Expression Vector
CD533A-2
SBI
10 ug
EUR 653
Category: Lentiviral Technology
pCDH-UbC-MCS-EF1-Hygro cDNA Cloning and Expression Vector
CD615B-1
SBI
10 ug
EUR 635
Category: Lentiviral Technology
pCDH-UbC-MCS-IRES-GFP cDNA Cloning and Expression Vector
CD630A-1
SBI
10 ug
EUR 653
Category: Lentiviral Technology
pCDH-CMV-MCS-EF1-Puro cDNA Cloning and Expression Vector
CD510B-1
SBI
10 ug
EUR 635
Category: Lentiviral Technology
pCDH-CMV-MCS-EF1-RFP cDNA Cloning and Expression Vector
CD512B-1
SBI
10 ug
EUR 635
Category: Lentiviral Technology
pCDH-CMV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector
CD513B-1
SBI
10 ug
EUR 635
Category: Lentiviral Technology
pCDH-CMV-MCS-EF1-Neo cDNA Cloning and Expression Vector
CD514B-1
SBI
10 ug
EUR 635
Category: Lentiviral Technology
pCDH-CMV-MCS-EF1-Hygro cDNA Cloning and Expression Vector
CD515B-1
SBI
10 ug
EUR 635
Category: Lentiviral Technology
pCDH-EF1-MCS*-T2A-GFP
CD526A-1
SBI
10 ug
EUR 653
Category: Lentiviral Technology
pAAVK-EF1? -MCS-T2A-EGFP AAVanced Cloning and Expression Vector
AAV526A-1
SBI
10 ug
EUR 677
pAAVK-EF1? -MCS-T2A-Puro AAVanced Cloning and Expression Vector
AAV527A-1
SBI
10 ug
EUR 677
pAAVK-EF1? ?MCS-T2A-mRFP AAVanced Cloning and Expression Vector
AAV528A-1
SBI
10 ug
EUR 677
pCDH-CMV-MCS-EF1-RFP+Puro cDNA Cloning and Expression Vector
CD516B-2
SBI
10 ug
EUR 635
Category: Lentiviral Technology
XStamp cloning and expression lentivector MSCV-Leader-MCS-C1C2-EF1-Puro
XSTP710PA-1
SBI
10 ug
EUR 797
pCDH-CuO-MCS-T2A-GFP co-inducible T2A lentivector
QM521A-1
SBI
10 ug
EUR 679
Category: Lentiviral Technology
pCDH-CuO-MCS-T2A-RFP co-inducible T2A lentivector
QM522A-1
SBI
10 ug
EUR 679
Category: Lentiviral Technology
pCDH-Cuo-MCS-EF1-CymR-T2A-Puro
QM800A-1
SBI
10 ug
EUR 1310
Category: Lentiviral Technology
pSIH1-H1-copGFP shRNA Cloning and Expression Vector
SI501A-1
SBI
10 ug
EUR 567
Category: Lentiviral Technology
pCDH-CMV-MCS2 cDNA Cloning and Expression Vector
CD501A-1
SBI
10 ug
EUR 544
Category: Lentiviral Technology
pAAVK-EF1? -MCS AAVanced Cloning and Expression Vector
AAV502A-1
SBI
10 ug
EUR 677
pCDF1-MCS2-EF1-copGFP cDNA Cloning and Expression Vector
CD111B-1
SBI
10 ug
EUR 635
Category: Lentiviral Technology
pCDH-CuO-MCS empty cloning lentivector
QM500A-1
SBI
10 ug
EUR 597
Category: Lentiviral Technology
BLIV 2.0 Reporter: MSCV-Luciferase-EF1a-copGFP-T2A-Puro Lentivector Plasmid
BLIV713PA-1
SBI
10 ug
EUR 803
Category: Bioluminescent Imaging
pCDH-CMV-MCS-EF1-copGFP cDNA Clon/Exp Vector [pre-packaged virus]
CD511VB-1
SBI
>1 x 10^6 IFUs
EUR 786
Category: Lentiviral Technology
pCDH-CMV-MCS-EF1-copGFP+Puro cDNA Clon/Exp Vector [pre-packaged]
CD513VB-1
SBI
>1 x 10^6 IFUs
EUR 786
Category: Lentiviral Technology
Scramble control hairpin in pCDH-CMV-MCS-EF1-copGFP (CD511B-1)
PMIRH000PA-1
SBI
Bacterial Streak
EUR 656
PB-CMV-MCS-EF1-Puro cDNA cloning and expression vector
PB510B-1
SBI
10 ug
EUR 702
Category: PiggyBac Technology
PB-CMV-MCS-EF1-GFP cDNA cloning and expression vector
PB511B-1
SBI
10 ug
EUR 702
Category: PiggyBac Technology
PB-CMV-MCS-EF1-RFP cDNA cloning and expression vector
PB512B-1
SBI
10 ug
EUR 702
Category: PiggyBac Technology
PB-CMV-MCS-EF1-GreenPuro cDNA cloning and expression vector
PB513B-1
SBI
10 ug
EUR 702
Category: PiggyBac Technology
PB-CMV-MCS-EF1-RedPuro cDNA Cloning and Expression Vector
PB514B-2
SBI
10 ug
EUR 702
Category: PiggyBac Technology
PB-EF1-MCS-IRES-GFP cDNA cloning and expression vector
PB530A-2
SBI
10 ug
EUR 702
Category: PiggyBac Technology
PB-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector
PB531A-2
SBI
10 ug
EUR 702
Category: PiggyBac Technology
PB-EF1-MCS-IRES-Neo cDNA cloning and expression vector
PB533A-2
SBI
10 ug
EUR 702
Category: PiggyBac Technology
PB-CMV-GreenPuro-H1-MCS shRNA cloning and expression vector
PBSI505A-1
SBI
10 ug
EUR 702
Category: PiggyBac Technology
PB-EF1-GreenPuro-H1-MCS shRNA cloning and expression vector
PBSI506A-1
SBI
10 ug
EUR 702
Category: PiggyBac Technology
XPack MSCV-XP-MCS-EF1-Puro Cloning Lentivector
XPAK710PA-1
SBI
10 ug
EUR 797
BLIV 2.0 Reporter: MSCV-Luciferase-EF1a-copGFP-T2A-Puro Pre-packaged Virus
BLIV713VA-1
SBI
>2 x10^6 IFUs
EUR 803
Category: Bioluminescent Imaging
pAAV-MCS Expression Vector
VPK-410
Cell Biolabs
10 µg
EUR 647
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.
pscAAV-MCS Expression Vector
VPK-430
Cell Biolabs
10 µg
EUR 647
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.
T2A-GFP Co-Expression HR Targeting Vector [T2A-GFP-pA-loxP-EF1?-RFP-T2A-Puro-pA-LoxP-MCS]
HR130PA-1
SBI
10 ug
EUR 1023
pCDH-CMV-MCS-EF1-copGFP cDNA Clon/Exp Vector [pre-packaged Ultra-High Titer virus]
CD511VB-UHT
SBI
>1x10^9 IFUs
EUR 1041
Category: Lentiviral Technology
pAAV-MCS Promoterless Expression Vector
VPK-411
Cell Biolabs
10 µg
EUR 647
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.
pMC.CMV-GFP-T2A-Puro-H1-MCS (shRNA expression parental minicircle plasmid)
MNSI505A-1
SBI
10 ug
EUR 847
Category: Minicircle Technology
pMC.EF1-GFP-T2A-Puro-H1-MCS (shRNA expression parental minicircle plasmid)
MNSI506A-1
SBI
10 ug
EUR 847
Category: Minicircle Technology
GFP-T2A-Luciferase Co-Expression HR Targeting Vector [GFP-T2A-Luc-pA-loxP-EF1?-RFP-T2A-Puro-pA-LoxP-MCS]
HR150PA-1
SBI
10 ug
EUR 1023
pCDH-EF1-CymR-T2A-Puro (plasmid)
QM200PA-2
SBI
10 ug
EUR 597
Category: Lentiviral Technology
pCDH-EF1-CymR-T2A-Puro (virus)
QM200VA-2
SBI
>1 x 10^6 IFUs
EUR 636
Category: Lentiviral Technology
pCDH-EF1-CymR-T2A-RFP (plasmid)
QM300PA-1
SBI
10 ug
EUR 597
Category: Lentiviral Technology
pCDH-EF1-CymR-T2A-RFP (virus)
QM300VA-1
SBI
>1 x 10^6 IFUs
EUR 636
Category: Lentiviral Technology
pCDH-EF1-CymR-T2A-Neo (plasmid)
QM400PA-2
SBI
10 ug
EUR 581
Category: Lentiviral Technology
pCDH-EF1-CymR-T2A-Neo (virus)
QM400VA-2
SBI
>1 x 10^6 IFUs
EUR 620
Category: Lentiviral Technology
PB-Cuo-MCS-IRES-GFP-EF1-CymR-Puro Inducible cDNA Cloning and Expression Vector
PBQM812A-1
SBI
10 ug
EUR 1096
Category: PiggyBac Technology
MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid
CASLV120PA-1
SBI
10 ug
EUR 697
pCDH-CuO-MCS-IRES-GFP-EF1-CymR-T2A-Puro All-in-one inducible IRES lentivector
QM812B-1
SBI
10 ug
EUR 1310
Category: Lentiviral Technology
pCDF1-MCS1 cDNA Cloning and Expression Vector
CD100A-1
SBI
10 ug
EUR 544
Category: Lentiviral Technology
pCDH-CuO-MCS-EF1-GFP+Puro dual promoter and dual marker lentivector
QM513B-1
SBI
10 ug
EUR 679
Category: Lentiviral Technology
pCDH-CuO-MCS-EF1-RFP+Puro dual promoter and dual marker lentivector
QM516B-2
SBI
10 ug
EUR 679
Category: Lentiviral Technology
pCDH-CuO-MCS-EF1-GFP dual promoter lentivector
QM511B-1
SBI
10 ug
EUR 679
Category: Lentiviral Technology
pCDH-CuO-MCS-EF1-RFP dual promoter lentivector
QM512B-1
SBI
10 ug
EUR 679
Category: Lentiviral Technology
Constitutive (CAGs promoter) EEV cloning and expression vector
EEV600A-1
SBI
10 ug
EUR 677
Category: Episomal Vectors
Cumate-Inducible (CuO) EEV cloning and expression vector
EEV610A-1
SBI
10 ug
EUR 677
Category: Episomal Vectors
pSIH1-H1-Puro shRNA Cloning and Expression Vector
SI500A-1
SBI
10 ug
EUR 567
Category: Lentiviral Technology
pSIH1-H1-H2Kk shRNA Cloning and Expression Vector
SI502A-1
SBI
10 ug
EUR 567
Category: Lentiviral Technology
pCDH-Cuo-RFP-T2A-GFP (positive control plasmid)
QM350PA-1
SBI
10 ug
EUR 597
Category: Lentiviral Technology
pCDH-Cuo-RFP-T2A-GFP (positive control virus)
QM350VA-1
SBI
>1 x 10^6 IFUs
EUR 636
Category: Lentiviral Technology
pCDH-CuO-MCS-IRES-GFP co-inducible IRES lentivector
QM530A-2
SBI
10 ug
EUR 679
Category: Lentiviral Technology
pCDH-CuO-MCS-IRES-RFP co-inducible IRES lentivector
QM531A-2
SBI
10 ug
EUR 679
Category: Lentiviral Technology
XPack MSCV-XP-GFP-EF1-Puro Expression Lentivector
XPAK730PA-1
SBI
10 ug
EUR 826
XPack MSCV-XP-GFP-EF1-Puro Expression Lentivirus
XPAK730VA-1
SBI
>1 x 10^6 IFUs
EUR 826
XPack MSCV-XP-Luciferase-EF1-Puro Expression Lentivector
XPAK732PA-1
SBI
10 ug
EUR 826
XPack MSCV-XP-Luciferase-EF1-Puro Expression Lentivirus
XPAK732VA-1
SBI
>1 x 10^6 IFUs
EUR 826
pCDH-EF1a-Piranha-T2A-Puro virus (>1x10^6 IFUs)
PTPD527VA-1
SBI
>1x10^6 IFUs
EUR 697
MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV120PA-KIT
SBI
1 Kit
EUR 1132
Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid
CASLV220PA-1
SBI
10 ug
EUR 697
Minicircle Dual Reporter: MSCV-RFP-T2A-Luciferase DNA (30 ug)
BLIV506MC-1
SBI
30 ug
EUR 716
Category: Bioluminescent Imaging
Minicircle Dual Reporter: MSCV-GFP-T2A-Luciferase DNA (30 ug)
BLIV507MC-1
SBI
30 ug
EUR 716
Category: Bioluminescent Imaging
pCDH-CMV-MCS-EF1-aRFP cDNA Clon/Exp Vector [pre-packaged]
CD512VA-1
SBI
>1 x 10^6 IFUs
EUR 786
Category: Lentiviral Technology
pFC-EF1-MCS-pA-PGK-RFP-T2A-Puro PhiC31 Donor Vector
FC550A-1
SBI
10ug
EUR 773
Category: PhiC31 Integrase Tools
pFC-PGK-MCS-pA-EF1-GFP-T2A-Puro PhiC31 Donor Vector
FC551A-1
SBI
10ug
EUR 773
Category: PhiC31 Integrase Tools
pAAVK-EF1?-MCS1-CMV-MCS2 AAVanced Cloning and Expression Vector
AAV503A-1
SBI
10 ug
EUR 677
pAAVK-EF1?-MCS1-CMV-EGFP AAVanced Cloning and Expression Vector
AAV536A-1
SBI
10 ug
EUR 677
pAAVK-EF1?-MCS1-CMV-Puro AAVanced Cloning and Expression Vector
AAV537A-1
SBI
10 ug
EUR 677
pAAVK-EF1?-MCS1-CMV-mRFP AAVanced Cloning and Expression Vector
AAV538A-1
SBI
10 ug
EUR 677
Minicircle Dual Reporter: MSCV-RFP-T2A-Luciferase Parental Plasmid (10 ug)
BLIV506MN-1
SBI
10 ug
EUR 972
Category: Bioluminescent Imaging
Minicircle Dual Reporter: MSCV-GFP-T2A-Luciferase Parental Plasmid (10 ug)
BLIV507MN-1
SBI
10 ug
EUR 972
Category: Bioluminescent Imaging
pCDH-CMV-MCS-EF1-RFP+Puro cDNA Clon/Exp Vector [pre-packaged]
CD516VB-2
SBI
>1 x 10^6 IFUs
EUR 772
Category: Lentiviral Technology
pCDF1-MCS2-EF1-Puro cDNA Cloning and Expression Lentivector
CD110B-1
SBI
10 ug
EUR 635
Category: Lentiviral Technology
MSCV-hspCas9-T2A-Puro SmartNuclease Pre-Packaged Lentiviral Particles [>10^6 IFUs]
CASLV120VA-1
SBI
2 x 25ul
EUR 697
pCDH-CMV-Piranha-EF1-GFP-T2A-Puro virus (>1x10^6 IFUs)
PTPD513VA-1
SBI
>1x10^6 IFUs
EUR 697
pCDH-CMV-MCS-EF1-Puro cDNA Vector cDNA Clon/Exp Vector [pre-packaged virus]
CD510VB-1
SBI
>1 x 10^6 IFUs
EUR 786
Category: Lentiviral Technology
pMC.CMV-GFP-H1-MCS (shRNA expression parental minicircle plasmid)
MNSI500A-1
SBI
10 ug
EUR 847
Category: Minicircle Technology
Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
CASLV220PA-KIT
SBI
1 Kit
EUR 1132
MSCV-hspCas9-T2A-Puro-H1-gRNA linearized all-in-one SmartNuclease Lentivector Plasmid
CASLV320PA-1
SBI
10 rxn
EUR 805
MSCV-Mouse Foxp3-EF1-GFP-T2A-Puro Stable Jurkat cell line (Puro resistant)
TCL110C-1
SBI
5 x 10^6 cells/ml
EUR 1682
Category: T Cell Products
MSCV-Human Foxp3-EF1-GFP-T2A-Puro Stable Jurkat cell line (Puro resistant)
TCL210C-1
SBI
5 x 10^6 cells/ml
EUR 1682
Category: T Cell Products
MSCV-Mouse RORgt-EF1-GFP-T2A-Puro Stable Jurkat cell line (Puro resistant)
TCL310C-1
SBI
5 x 10^6 cells/ml
EUR 1682
Category: T Cell Products
MSCV-Human RORgt-EF1-GFP-T2A-Puro Stable Jurkat cell line (Puro resistant)
TCL410C-1
SBI
5 x 10^6 cells/ml
EUR 1682
Category: T Cell Products
XPack CMV-XP-MCS-EF1-Puro Cloning Lentivector
XPAK510PA-1
SBI
10 ug
EUR 797
GFP-Fusion HR Targeting Vector [GFP-pA-LoxP-EF1?-RFP-T2A-Puro-pA-LoxP-MCS]
HR120PA-1
SBI
10 ug
EUR 1023
GFP-Fusion HR Targeting Vector [GFP-pA-LoxP-EF1a-RFP-T2A-Hygro-pA-LoxP-MCS]
HR220PA-1
SBI
10 ug
EUR 1023
Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Pre-Packaged Lentiviral Particles [>10^6 IFUs]
CASLV220VA-1
SBI
2 x 25ul
EUR 697
PB-Cuo-shMCS-IRES-GFP-EF1-CymR-Puro Inducible shRNA Cloning and Expression Vector
PBQMSH812A-1
SBI
10 ug
EUR 1096
Category: PiggyBac Technology
MSCV-hspCas9-H1-gRNA SmartNuclease vector
CAS960A-1
SBI
10 ug
EUR 805
PinPoint-HR attP Placement Vector ? Empty MCS for cloning HR Arms
PIN400A-1
SBI
10ug
EUR 1268
Category: PinPoint Integrase Tools
Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro-H1-gRNA linearized all-in-one SmartNuclease Lentivector Plasmid
CASLV420PA-1
SBI
10 rxn
EUR 805
MCS ORF Vector (Human) (pORF)
ORF023399
ABM
1.0 ug DNA
Ask for price
pMC.CMV-MCS-SV40polyA
MN501A-1
SBI
10 ug
EUR 735
Category: Minicircle Technology
pMC.EF1-MCS-SV40polyA
MN502A-1
SBI
10 ug
EUR 735
Category: Minicircle Technology
pPS-MSCV-GFP-RFP (plasmid)
LV605PA-1
SBI
10 ug
EUR 656
Category: Lentiviral Technology
PCDH-X/Y Antibody
45549-100ul
SAB
100ul
EUR 252
PCDH-X/Y Antibody
45549-50ul
SAB
50ul
EUR 187
PCDH-X/Y Antibody
DF8867
Affbiotech
200ul
EUR 304
Description: PCDH-X/Y Antibody detects endogenous levels of total PCDH-X/Y.
PCDH-X / Y Antibody
20-abx218014
Abbexa
Shipped within 5-10 working days.
pORB-MCS-sIRES-VSVG
ABP-BM-sIRESVG
Allele Biotech
5 ug
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Product line: Sapphire Baculo3MamBrand:
CONCLUSION Recombinant eukaryotic expression vector pCDH-FAP successfully constructed. These results provide a basis for further study on the function of FAP in vitro.