Neuronal differentiation of hair-follicle-bulge-derived stem cells co-cultured with mouse cochlear modiolus explants.

Neuronal differentiation of hair-follicle-bulge-derived stem cells co-cultured with mouse cochlear modiolus explants.

Stem cell-based repair auditory neurons may represent an attractive therapeutic option to restore sensorineural hearing loss. stem cells of hair-follicle-bulge-derived (HFBSCs) are promising candidates for this type of therapy, because they (1) have the nature of migration, allowing migration after transplantation, (2) can differentiate into sensory neurons and glial cells, and (3) can easily be harvested in relatively high amounts. However, HFBSCs never used for this purpose.

We hypothesize that HFBSCs can be used to repair the auditory nerve cell-based and we have researched their migration and incorporation into the cochlear modiolus explant and subsequent differentiation of them. Modiolus explants derived from adult wild type mice were cultured in the presence of HFBSCs EF1α-copGFP-transduced, constitutively expressing green fluorescent protein copepods (copGFP). Also, explants modiolus without hair cells were co-cultured with DCX-copGFP HFBSCs-transduced, indicating copGFP on doublecortin expression during neuronal differentiation.

HFBSC speed migration towards modiolus explants is calculated, and after two weeks, co-cultures were fixed and processed for immunohistochemical staining. EF1α-copGFP HFBSC rapid migration speed: 80.5 ± 6.1 m / h. After arriving in explants, cells form a pattern fasciculus and change their phenotype into neural cell types Atoh1-positive. DCX-copGFP HFBSCs green-fluorescent after integration into the explants, justify the differentiation of neurons from cells. These results indicate that HFBSC derived progenitor neuronal migration and can integrate into the cochlear modiolus explants, while adapting their phenotypes depending on the micro environment. Thus, HFBSCs demonstrate the potential to be employed in cell-based therapies to repair the auditory nerve.

The immunomodulatory and self-renewable human features adipose-derived mesenchymal stem cells (HAD-MSCs) mark their importance in regenerative medicine. Interleukin (IL) -23 as proinflammatory cytokines Hitting the regulatory T cells and promote the response of T helper 17 and T helper 1 cells. This initiation pathways of inflammation and immunosuppression in several autoimmune diseases.

 Neuronal differentiation of hair-follicle-bulge-derived stem cells co-cultured with mouse cochlear modiolus explants.
Neuronal differentiation of hair-follicle-bulge-derived stem cells co-cultured with mouse cochlear modiolus explants.

[Construction of eukaryotic expression vector fibroblast activation protein and the formation of stable over-expression in squamous cell carcinoma of the mouth].

OBJECTIVE
This study aimed to construct vectors fibroblast activation protein (FAP) over-expression lentivinus to investigate transfection in cell lines and build a stable FAP SCC9 over-expression of oral squamous cell lines.


METHOD
The cDNA of the FAP gene from squamous cell carcinoma of the oral tissues (OSCC) was amplified by polymerase chain reaction (PCR) and subkloning into eukaryotic expression vector pCDH-CMV-MCS-EF1-copGFP. Recombinant plasmids were lined up and then transfected into cell lines SCC9. Further, cell lines which over-expressed FAP SCC9 stable founded by fluorescence activated cell sorting (FACS). Expression of green fluorescent protein (GFP) was detected with a fluorescence microscope, and over-expression of FAP identified by real-time PCR and Western blot.


RESULTS
FAP gene amplified by PCR and then cloned into a vector, whose sequence is identical to that in GenBank. GFP was expressed in transfected cells. Furthermore, FAP over-expression in transfected cells was detected by real-time PCR and Western blot.

pCDH-MSCV-MCS-EF1-Puro cDNA Cloning and Expression Vector

CD710B-1 10 ug
EUR 572

pCDH-MSCV-MCS-IRES-GFP cDNA Cloning and Expression Vector

CD731B-1 10 ug
EUR 590

pCDH-CMV-MCS-EF1-copGFP cDNA Cloning and Expression Vector

CD511B-1 10 ug
EUR 572

pCDH- MSCV- MCS- EF1- CopGFP- T2A- Puro

PVT11079 2 ug
EUR 444

pCDH-MSCV-MCS-EF1-GFP+Puro cDNA Cloning and Expression Vector

CD713B-1 10 ug
EUR 572

pCDH-CMV-MCS cDNA Cloning and Expression Vector

CD500B-1 10 ug
EUR 483

pCDH-EF1-MCS cDNA Cloning and Expression Vector

CD502A-1 10 ug
EUR 483

pCDH-EF1-MCS-T2A-RFP (PGK-Puro) Cloning and Expression Vector

CD822A-1 10 ug
EUR 579

pCDH-EF1-MCS-T2A-GFP (PGK-Puro) Cloning and Expression Vector

CD823A-1 10 ug
EUR 579

pCDH-EF1-MCS-(PGK-GFP-T2A-Puro) Cloning and Expression Vector

CD813A-1 10 ug
EUR 579

pCDH-EF1-MCS-T2A-Puro cDNA Cloning and Expression Vector 10 µg

CD527A-1 10 ug
EUR 590

pCDH-EF1-MCS-(PGK-GFP) Cloning and Expression Vector

CD811A-1 10 ug
EUR 579

pCDH-EF1-MCS-(PGK-Puro) Cloning and Expression Vector

CD810A-1 10 ug
EUR 579

pCDH-EF1-MCS-BGH-PGK-GFP-T2A-Puro cDNA Cloning and Expression Vector

CD550A-1 10 ug
EUR 563

pCDH-CMV-MCS-EF1-RFP cDNA Cloning and Expression Vector

CD512B-1 10 ug
EUR 572

pCDH-CMV-MCS-EF1-Neo cDNA Cloning and Expression Vector

CD514B-1 10 ug
EUR 572

pCDH-CMV-MCS-EF1-Puro cDNA Cloning and Expression Vector

CD510B-1 10 ug
EUR 572

pCDH-EF1-MCS-IRES-GFP cDNA Cloning and Expression Vector

CD530A-2 10 ug
EUR 590

pCDH-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector

CD531A-2 10 ug
EUR 590

pCDH-EF1-MCS-IRES-Neo cDNA Cloning and Expression Vector

CD533A-2 10 ug
EUR 590

pCDH-UbC-MCS-IRES-GFP cDNA Cloning and Expression Vector

CD630A-1 10 ug
EUR 590

pCDH-CMV-MCS-EF1-Hygro cDNA Cloning and Expression Vector

CD515B-1 10 ug
EUR 572

pCDH-UbC-MCS-EF1-Hygro cDNA Cloning and Expression Vector

CD615B-1 10 ug
EUR 572

pCDH-EF1-MCS-IRES-Puro cDNA Cloning and Expression Vector

CD532A-2 10 ug
EUR 590

pCDH-CMV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector

CD513B-1 10 ug
EUR 572

pCDH-CMV-MCS-EF1-RFP+Puro cDNA Cloning and Expression Vector

CD516B-2 10 ug
EUR 572

pAAVK-EF1α -MCS-T2A-EGFP AAVanced Cloning and Expression Vector

AAV526A-1 10 ug
EUR 614

pAAVK-EF1α -MCS-T2A-Puro AAVanced Cloning and Expression Vector

AAV527A-1 10 ug
EUR 614

pAAVK-EF1α –MCS-T2A-mRFP AAVanced Cloning and Expression Vector

AAV528A-1 10 ug
EUR 614

pCDH-CMV-MCS2 cDNA Cloning and Expression Vector

CD501A-1 10 ug
EUR 483

pCDH- EF1- MCS- T2A- copGFP

PVT2336 2 ug
EUR 390

PB-MSCV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector

PB713B-1 10 ug
EUR 638

pAAVK-EF1α -MCS AAVanced Cloning and Expression Vector

AAV502A-1 10 ug
EUR 614

XStamp cloning and expression lentivector MSCV-Leader-MCS-C1C2-EF1-Puro

XSTP710PA-1 10 ug
EUR 730

pCDF1-MCS2-EF1-copGFP cDNA Cloning and Expression Vector

CD111B-1 10 ug
EUR 572

pSIH1-H1-copGFP shRNA Cloning and Expression Vector

SI501A-1 10 ug
EUR 507

PB-CMV-MCS-EF1-GFP cDNA cloning and expression vector

PB511B-1 10 ug
EUR 638

PB-CMV-MCS-EF1-RFP cDNA cloning and expression vector

PB512B-1 10 ug
EUR 638

pCDH- CMV- MCS- EF1- CopGFP- T2A- Puro

PVT2333 2 ug
EUR 390

PB-CMV-MCS-EF1-Puro cDNA cloning and expression vector

PB510B-1 10 ug
EUR 638

PB-EF1-MCS-IRES-GFP cDNA cloning and expression vector

PB530A-2 10 ug
EUR 638

PB-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector

PB531A-2 10 ug
EUR 638

PB-EF1-MCS-IRES-Neo cDNA cloning and expression vector

PB533A-2 10 ug
EUR 638

PB-CMV-MCS-EF1-RedPuro cDNA Cloning and Expression Vector

PB514B-2 10 ug
EUR 638

pCDF1-MCS1 cDNA Cloning and Expression Vector

CD100A-1 10 ug
EUR 483

PB-CMV-GreenPuro-H1-MCS shRNA cloning and expression vector

PBSI505A-1 10 ug
EUR 638

PB-EF1-GreenPuro-H1-MCS shRNA cloning and expression vector

PBSI506A-1 10 ug
EUR 638

PB-CMV-MCS-EF1-GreenPuro cDNA cloning and expression vector

PB513B-1 10 ug
EUR 638

pSIH1-H1-Puro shRNA Cloning and Expression Vector

SI500A-1 10 ug
EUR 507

pSIH1-H1-H2Kk shRNA Cloning and Expression Vector

SI502A-1 10 ug
EUR 507

Cumate-Inducible (CuO) EEV cloning and expression vector

EEV610A-1 10 ug
EUR 614

PB-CuO-CMV-MCS-EF1α-CymR-Puro Inducible cDNA Cloning and Expression Vector

PBQM800A-1 10 µg
EUR 921

Constitutive (CAGs promoter) EEV cloning and expression vector

EEV600A-1 10 ug
EUR 614

PB-Cuo-MCS-IRES-GFP-EF1-CymR-Puro Inducible cDNA Cloning and Expression Vector

PBQM812A-1 10 ug
EUR 1023

pAAVK-EF1α-MCS1-CMV-MCS2 AAVanced Cloning and Expression Vector

AAV503A-1 10 ug
EUR 614

pAAVK-EF1α-MCS1-CMV-EGFP AAVanced Cloning and Expression Vector

AAV536A-1 10 ug
EUR 614

pAAVK-EF1α-MCS1-CMV-Puro AAVanced Cloning and Expression Vector

AAV537A-1 10 ug
EUR 614

pAAVK-EF1α-MCS1-CMV-mRFP AAVanced Cloning and Expression Vector

AAV538A-1 10 ug
EUR 614

pCDH- CMV- MCS- EF1- copGFP Plasmid

PVT2334 2 ug
EUR 390

pCDF1-MCS2-EF1-Puro cDNA Cloning and Expression Lentivector

CD110B-1 10 ug
EUR 572

PB-Cuo-shMCS-IRES-GFP-EF1-CymR-Puro Inducible shRNA Cloning and Expression Vector

PBQMSH812A-1 10 ug
EUR 1023

pLenti-CAG-MCS-PGK-copGFP Lentiviral Expression Vector

LV310 10 µg
EUR 416.5

pLenti-SFFV-MCS-EF1-copGFP Lentiviral Expression Vector

LV460 10 µg
EUR 416.5

pAAV-MCS Expression Vector

VPK-410 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pscAAV-MCS Expression Vector

VPK-430 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pLenti-MCS-EF1-GFP-T2A-puro Lentiviral Expression Vector

LV011 10 µg
EUR 416.5

pLenti-MCS-EF1-RFP-T2A-puro Lentiviral Expression Vector

LV021 10 µg
EUR 416.5

baculoCOMPLETE protein expression kit

400100 5 reactions
EUR 874.2

pLenti-EF1-MCS-PGK-GFP-T2A-PURO Lentiviral Expression Vector

LV211 10 µg
EUR 416.5

pLenti-EF1-MCS-PGK-GFP-T2A-Neo Lentiviral Expression Vector

LV212 10 µg
EUR 416.5

pLenti-EF1-MCS-PGK-RFP-T2A-PURO Lentiviral Expression Vector

LV221 10 µg
EUR 416.5

pLenti-CMV-MCS-EF1α-GFP-T2A-PURO Lentiviral Expression Vector

LV111 10 µg
EUR 416.5

pLenti-CMV-MCS-EF1α-RFP-T2A-PURO Lentiviral Expression Vector

LV121 10 µg
EUR 416.5

pLenti-EF1-MCS-PGK-GFP-T2A-Hygro Lentiviral Expression Vector

LV213 10 µg
EUR 416.5

pLenti-CAG-MCS-PGK-coGFP-T2A-Puro Lentiviral Expression Vector

LV311 10 µg
EUR 416.5

pLenti-CAG-MCS-PGK-coGFP-T2A-NEO Lentiviral Expression Vector

LV312 10 µg
EUR 416.5

pLenti-CAG-MCS-PGK-coGFP-T2A-HygR Lentiviral Expression Vector

LV313 10 µg
EUR 416.5

pLenti-CAG-MCS-EF1-coGFP-T2A-PURO Lentiviral Expression Vector

LV361 10 µg
EUR 416.5

pLenti-SFFV-MCS-PGK-coGFP-T2A-NEO Lentiviral Expression Vector

LV412 10 µg
EUR 416.5

pLenti-MCS Lentiviral Expression Vector

LV000 10 µg
EUR 416.5

pAAV-MCS Promoterless Expression Vector

VPK-411 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pLenti-SFFV-MCS-PGK-coGFP-T2A-Puro Lentiviral Expression Vector

LV411 10 µg
EUR 416.5

pLenti-SFFV-MCS-PGK-coGFP-T2A-HygR Lentiviral Expression Vector

LV413 10 µg
EUR 416.5

pLenti-SFFV-MCS-EF1-coGFP-T2A-Puro Lentiviral Expression Vector

LV461 10 µg
EUR 416.5

pLenti-CAG-MCS-PGK-TdTomato-T2A-Puro Lentiviral Expression Vector

LV321 10 µg
EUR 416.5

pLenti-CAG-MCS-EF1-TdTomato-T2A-Puro Lentiviral Expression Vector

LV371 10 µg
EUR 416.5

pLenti-SFFV-MCS-PGK-TdTomato-T2A-Puro Lentiviral Expression Vector

LV421 10 µg
EUR 416.5

pLenti-SFFV-MCS-EF1-TdTomato-T2A-Puro Lentiviral Expression Vector

LV471 10 µg
EUR 416.5

XPack MSCV-XP-GFP-EF1-Puro Expression Lentivirus

XPAK730VA-1 >1 x 10^6 IFUs
EUR 759

XPack MSCV-XP-GFP-EF1-Puro Expression Lentivector

XPAK730PA-1 10 ug
EUR 759

pLenti-CMV-MCS Lentiviral Expression Vector

LV100 10 µg
EUR 416.5

pLenti-EF1-MCS Lentiviral Expression Vector

LV200 10 µg
EUR 416.5

pLenti-CAG-MCS Lentiviral Expression Vector

LV300 10 µg
EUR 416.5

pLenti-SFFV-MCS Lentiviral Expression Vector

LV400 10 µg
EUR 416.5

baculoCOMPLETE protein expression kit + baculoQUANT all-in-one

400101 5 + 100 reactions
EUR 1419.8

XPack MSCV-XP-Luciferase-EF1-Puro Expression Lentivector

XPAK732PA-1 10 ug
EUR 759

XPack MSCV-XP-Luciferase-EF1-Puro Expression Lentivirus

XPAK732VA-1 >1 x 10^6 IFUs
EUR 759

pLenti-MCS-EF1-GFP Lentiviral Expression Vector

LV010 10 µg
EUR 416.5

pLenti-MCS-PGK-PURO Lentiviral Expression Vector

LV001 10 µg
EUR 416.5

pLenti-EF1-MCS-PGK-Neo Lentiviral Expression Vector

LV202 10 µg
EUR 416.5

pLenti-EF1-MCS-PGK-GFP Lentiviral Expression Vector

LV210 10 µg
EUR 416.5

pLenti-EF1-MCS-PGK-RFP Lentiviral Expression Vector

LV220 10 µg
EUR 416.5

pLenti-CAG-MCS-PGK-NEO Lentiviral Expression Vector

LV302 10 µg
EUR 416.5

pLenti-CAG-MCS- EF1-coGFP Lentiviral Expression Vector

LV360 10 µg
EUR 416.5

pLenti-CMV-MCS-EF1α-GFP Lentiviral Expression Vector

LV110 10 µg
EUR 416.5

pLenti-CMV-MCS-EF1α-RFP Lentiviral Expression Vector

LV120 10 µg
EUR 416.5

pLenti-EF1-MCS-PGK-PURO Lentiviral Expression Vector

LV201 10 µg
EUR 416.5

pLenti-CAG-MCS-PGK-Puro Lentiviral Expression Vector

LV301 10 µg
EUR 416.5

pLenti-CAG-MCS-PGK-HygR Lentiviral Expression Vector

LV303 10 µg
EUR 416.5

pLenti-CAG-MCS-EF1-PURO Lentiviral Expression Vector

LV351 10 µg
EUR 416.5

pLenti-SFFV-MCS-PGK-NEO Lentiviral Expression Vector

LV402 10 µg
EUR 416.5

pLenti-SFFV-MCS-PGK-GFP Lentiviral Expression Vector

LV410 10 µg
EUR 416.5

pLenti-CMV-MCS-EF1α-PURO Lentiviral Expression Vector

LV101 10 µg
EUR 416.5

pLenti-EF1-MCS-PGK-Hygro Lentiviral Expression Vector

LV203 10 µg
EUR 416.5

pLenti-SFFV-MCS-PGK-Puro Lentiviral Expression Vector

LV401 10 µg
EUR 416.5

pLenti-SFFV-MCS-EF1-Puro Lentiviral Expression Vector

LV451 10 µg
EUR 416.5

pLenti-SFFV-MCS-PGK-Hygro Lentiviral Expression Vector

LV403 10 µg
EUR 416.5

pLenti-CAG-MCS-PGK-TdTomato Lentiviral Expression Vector

LV320 10 µg
EUR 416.5

pLenti-CAG-MCS-EF1-TdTomato Lentiviral Expression Vector

LV370 10 µg
EUR 416.5

pLenti-SFFV-MCS-PGK-TdTomato Lentiviral Expression Vector

LV420 10 µg
EUR 416.5

pLenti-SFFV-MCS-EF1-TdTomato Lentiviral Expression Vector

LV470 10 µg
EUR 416.5

pCDH- CMV- mCherry- EF1- CopGFP- Puro

PVT11078 2 ug
EUR 444

CNGA2 Expression vector

60642 20 µg
EUR 900
Description: The CNGA2 expression vector is designed to express human cyclic nucleotide-gated cation channel 2 (CNGA2) in mammalian cells.

pYLEX1 - Expression Vector

FYY203-5MG 5mg Ask for price

ACE2 Expression Lentivector, pCDH-CMV-ACE2-EF1α-Puro (Plasmid)

CVD19-100PA-1 10 µg
EUR 728

NRP1 Expression Lentivector, pCDH-CMV-NRP1-EF1α-Neo (Plasmid)

CVD19-140PA-1 10 µg
EUR 728

Rac1 Expression Vector Set

STA-454 1 kit
EUR 1765.2
Description: Rac1 Expression Vector Set contains 3 vectors: Rac wild type, T17N dominant negative mutant, and G12V constitutively active mutant.

RhoA Expression Vector Set

STA-456 1 kit
EUR 1765.2
Description: RhoA Expression Vector Set contains 3 vectors: RhoA wild type, T19N dominant negative mutant, and G14V constitutively active mutant.

Cdc42 Expression Vector Set

STA-455 1 kit
EUR 1765.2
Description: Cdc42 Expression Vector Set contains 3 vectors: Cdc42 wild type, T17N dominant negative mutant, and Q61L constitutively active mutant.

pMC.CMV-GFP-T2A-Puro-H1-MCS (shRNA expression parental minicircle plasmid)

MNSI505A-1 10 ug
EUR 779

pMC.EF1-GFP-T2A-Puro-H1-MCS (shRNA expression parental minicircle plasmid)

MNSI506A-1 10 ug
EUR 779

H-Ras Expression Vector Set

STA-457 1 kit
EUR 1765.2
Description: H-Ras Expression Vector Set contains 3 vectors: H-Ras wild type, T19N dominant negative mutant, and G12V constitutively active mutant.

pUC57-sgRNA expression vector

PVT18003 2 ug
EUR 309.6

ANPRA/Aequorin Expression vector

60641 20 µg
EUR 900
Description: The ANPRA/Aequorin expression vector is designed to co-express human atrial natriuretic peptide receptor A (ANPRA, also called natriuretic peptide receptor A/guanylate cyclase A) and jellyfish (Aequorea victoria) Aequorin in mammalian cells.

GFP-Rac1 Expression Vector Set

STA-450 1 kit
EUR 1939.2
Description: GFP-Rac1 Expression Vector Set contains 3 vectors: Rac wild type, T17N dominant negative mutant, and Q61L constitutively active mutant. Each vector also contains a GFP reporter sequence.

GFP-RhoA Expression Vector Set

STA-452 1 kit
EUR 1939.2
Description: GFP-RhoA Expression Vector Set contains 3 vectors: RhoA wild type, T19N dominant negative mutant, and Q63L constitutively active mutant. Each vector also contains a GFP reporter sequence.

GFP-Cdc42 Expression Vector Set

STA-451 1 kit
EUR 1939.2
Description: GFP-Cdc42 Expression Vector Set contains 3 vectors: Cdc42 wild type, T17N dominant negative mutant, and Q61L constitutively active mutant. Each vector also contains a GFP reporter sequence.

Active Rac1 Expression Vector Set

STA-458 1 kit
EUR 1765.2
Description: Active Rac1 Expression Vector Set contains 3 vectors expressing different constitutively active mutants of Rac1: Q61L, Q61L/F37A, and Q61L/Y40C.

pCMV-GFP-LC3 Expression Vector

CBA-401 100 µL
EUR 1243.2
Description: Expression vector contains a fusion of GFP and LC3. A separate GFP control vector is also included.

Tet-on circRNA Expression Vector

PVT14643 2 ug
EUR 843.6

pAAV-IRES-Neo Expression Vector

VPK-416 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-IRES-GFP Expression Vector

VPK-418 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-IRES-Bsd Expression Vector

VPK-419 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-IRES-Puro Expression Vector

VPK-415 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-IRES-Hygro Expression Vector

VPK-417 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

Active H-Ras Expression Vector Set

STA-459 1 kit
EUR 1765.2
Description: Active H-Ras Expression Vector Set contains 3 vectors expressing different constitutively active mutants of H-Ras: V12, V12S35, and V12C40.

pSMPUW-Neo Lentiviral Expression Vector

VPK-213 10 µg
EUR 748.8
Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer.

pSMPUW-Puro Lentiviral Expression Vector

VPK-212 10 µg
EUR 748.8
Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer.

pGreen- Puro- pSIH1- H1- copGFP- T2A- Puro

PVT11116 2 ug
EUR 444

pSMPUW-Hygro Lentiviral Expression Vector

VPK-214 10 µg
EUR 748.8
Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer.

XPack MSCV-XP-MCS-EF1-Puro Cloning Lentivector

XPAK710PA-1 10 ug
EUR 730

ACE2 Expression Lentivector, pCDH-CMV-ACE2-EF1α-Puro (Pre-packaged Lentivirus)

CVD19-100VA-1 >2x10^6 IFUs
EUR 728

NRP1 Expression Lentivector, pCDH-CMV-NRP1-EF1α-Neo (Pre-packaged Lentivirus)

CVD19-140VA-1 >2x10^6 IFUs
EUR 728

Baculovirus expression vector pAc-g-CH3

30R-AB001 5 ug
EUR 739.2
Description: Baculovirus cassette vector pAc-l-CH3 for the expression of human, humanized or chimeric IgG(lambda) in insect cells and secretion of assembled antibodies into the supernatant.

Exoenzyme C3 (Rho Inhibitor) Expression Vector

STA-460 1 kit
EUR 860.4
Description: This mammalian expression vector codes for Exoenzyme C3, a Rho inhibitor.

pSMPUW-GFP-LC3 Lentiviral Expression Vector

LTV-801 10 µg
EUR 1444.8
Description: Expression vector contains a fusion of GFP and LC3. A separate GFP control vector is also included.

pSMPUW-IRES-Neo Lentiviral Expression Vector

VPK-216 10 µg
EUR 748.8
Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer.

pSMPUW-IRES-Puro Lentiviral Expression Vector

VPK-215 10 µg
EUR 748.8
Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer.

pCDH-CuO-MCS empty cloning lentivector

QM500A-1 10 ug
EUR 534

pSMPUW-IRES-Hygro Lentiviral Expression Vector

VPK-217 10 µg
EUR 748.8
Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer.

Mouse IL-23 Enhanced Episomal Expression Vector

EEV651A-1 100 ug
EUR 812

pCDH- EF1- MCS- T2A- Puro Plasmid

PVT2335 2 ug
EUR 390

pMXs-miRNA-GFP/Puro Retroviral Expression Vector

RTV-017 10 ?g
EUR 748.8
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pSMPUW-IRES-Blasticidin Lentiviral Expression Vector

VPK-219 10 µg
EUR 748.8
Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer.

pSMPUW Universal Lentiviral Expression Vector (Promoterless)

VPK-211 10 µg
EUR 748.8
Description: Clone your gene of interest and a gene-specific promoter into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293T or 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer.

Excision only piggyBac Transposase expression vector, 50 rxns

PB220PA-1 10 ug
EUR 471

pMC.CMV-GFP-H1-MCS (shRNA expression parental minicircle plasmid)

MNSI500A-1 10 ug
EUR 779

Protein expression

CRO-4-Ind each Ask for price

YLEX Expression Kit

FYY201-1KT 1 kit Ask for price

SARS-CoV-2 Spike Glycoprotein Expression (S) Lentivector, pCDH-CMV-SARS-CoV-2-S-EF1α-Puro (Plasmid)

CVD19-130PA-1 10 µg
EUR 728

pCDH-EF1-MCS*-T2A-GFP

CD526A-1 10 ug
EUR 590

pCDH- CMV- MCS- EF1- turboRFP- T2A- Puro

PVT11077 2 ug
EUR 444

Super piggyBac Transposase expression vector (replaces PB200A-1), 50 rxns

PB210PA-1 10 ug
EUR 471

293 Expression MAX-1

EXP-711-11 1ml
EUR 214
Description: 293 Expression MAX-1 is expressed from - -.

pCDH-CMV-MCS-EF1-copGFP-T2A-Puro plasmid

PVT17271 2 ug
EUR 568.8

SARS-CoV-2 Nucleocapsid (N protein) Expression Lentivector, pCDH-CMV-SARS-CoV-2-N-EF1α-Puro (Plasmid)

CVD19-120PA-1 10 µg
EUR 728

Blunt E1 Expression Kit

abx098098-10rxns 10 rxns
EUR 560.4

Blunt E2 Expression Kit

abx098873-10rxns 10 rxns
EUR 560.4

RFP expression Adenovirus

AVP001 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made RFP expression adenovirus, provided in DMEM medium.

CFP expression Adenovirus

AVP002 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made CFP expression adenovirus, provided in DMEM medium.

GFP expression Adenovirus

AVP011 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made GFP expression adenovirus, provided in DMEM medium.

YFP expression Adenovirus

AVP012 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made YFP expression adenovirus, provided in DMEM medium.

BFP expression Adenovirus

AVP017 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made BFP expression adenovirus, provided in DMEM medium.

SARS-CoV-2 Humanized Spike Glycoprotein (S) Expression Lentivector, pCDH-CMV-SARS-CoV-2-hS-EF1α-Puro (Plasmid)

CVD19-135PA-1 10 µg
EUR 728

h ADA Expression Lentivirus

LVP923 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: ADA, containing a RFP-Blasticidin dual selection marker.

h IL6 Expression Lentivirus

LVP1144 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.

h CD4 Expression Lentivirus

LVP1152 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: CD4, containing a RFP-Blasticidin dual selection marker.

h IL4 Expression Lentivirus

LVP1161 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: IL4, containing a RFP-Blasticidin dual selection marker.

h IL7 Expression Lentivirus

LVP1162 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: IL7, containing a RFP-Blasticidin dual selection marker.

h KDR Expression Lentivirus

LVP1176 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: KDR, containing a RFP-Blasticidin dual selection marker.


CONCLUSION
Recombinant eukaryotic expression vector pCDH-FAP successfully constructed. These results provide a basis for further study on the function of FAP in vitro.

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