Neuronal differentiation of hair-follicle-bulge-derived stem cells co-cultured with mouse cochlear modiolus explants.

Neuronal differentiation of hair-follicle-bulge-derived stem cells co-cultured with mouse cochlear modiolus explants.

Stem cell-based repair auditory neurons may represent an attractive therapeutic option to restore sensorineural hearing loss. stem cells of hair-follicle-bulge-derived (HFBSCs) are promising candidates for this type of therapy, because they (1) have the nature of migration, allowing migration after transplantation, (2) can differentiate into sensory neurons and glial cells, and (3) can easily be harvested in relatively high amounts. However, HFBSCs never used for this purpose.

We hypothesize that HFBSCs can be used to repair the auditory nerve cell-based and we have researched their migration and incorporation into the cochlear modiolus explant and subsequent differentiation of them. Modiolus explants derived from adult wild type mice were cultured in the presence of HFBSCs EF1α-copGFP-transduced, constitutively expressing green fluorescent protein copepods (copGFP). Also, explants modiolus without hair cells were co-cultured with DCX-copGFP HFBSCs-transduced, indicating copGFP on doublecortin expression during neuronal differentiation.

HFBSC speed migration towards modiolus explants is calculated, and after two weeks, co-cultures were fixed and processed for immunohistochemical staining. EF1α-copGFP HFBSC rapid migration speed: 80.5 ± 6.1 m / h. After arriving in explants, cells form a pattern fasciculus and change their phenotype into neural cell types Atoh1-positive. DCX-copGFP HFBSCs green-fluorescent after integration into the explants, justify the differentiation of neurons from cells. These results indicate that HFBSC derived progenitor neuronal migration and can integrate into the cochlear modiolus explants, while adapting their phenotypes depending on the micro environment. Thus, HFBSCs demonstrate the potential to be employed in cell-based therapies to repair the auditory nerve.

The immunomodulatory and self-renewable human features adipose-derived mesenchymal stem cells (HAD-MSCs) mark their importance in regenerative medicine. Interleukin (IL) -23 as proinflammatory cytokines Hitting the regulatory T cells and promote the response of T helper 17 and T helper 1 cells. This initiation pathways of inflammation and immunosuppression in several autoimmune diseases.

 Neuronal differentiation of hair-follicle-bulge-derived stem cells co-cultured with mouse cochlear modiolus explants.
Neuronal differentiation of hair-follicle-bulge-derived stem cells co-cultured with mouse cochlear modiolus explants.

[Construction of eukaryotic expression vector fibroblast activation protein and the formation of stable over-expression in squamous cell carcinoma of the mouth].

OBJECTIVE
This study aimed to construct vectors fibroblast activation protein (FAP) over-expression lentivinus to investigate transfection in cell lines and build a stable FAP SCC9 over-expression of oral squamous cell lines.


METHOD
The cDNA of the FAP gene from squamous cell carcinoma of the oral tissues (OSCC) was amplified by polymerase chain reaction (PCR) and subkloning into eukaryotic expression vector pCDH-CMV-MCS-EF1-copGFP. Recombinant plasmids were lined up and then transfected into cell lines SCC9. Further, cell lines which over-expressed FAP SCC9 stable founded by fluorescence activated cell sorting (FACS). Expression of green fluorescent protein (GFP) was detected with a fluorescence microscope, and over-expression of FAP identified by real-time PCR and Western blot.


RESULTS
FAP gene amplified by PCR and then cloned into a vector, whose sequence is identical to that in GenBank. GFP was expressed in transfected cells. Furthermore, FAP over-expression in transfected cells was detected by real-time PCR and Western blot.

pCDH- EF1- MCS- T2A- copGFP

PVT2336 2 ug
EUR 390

pCDH-CMV-MCS-EF1-copGFP cDNA Cloning and Expression Vector

CD511B-1 10 ug
EUR 762

pCDH-MSCV-MCS-EF1-Puro cDNA Cloning and Expression Vector

CD710B-1 10 ug
EUR 762

pCDH-MSCV-MCS-EF1-GFP cDNA Cloning and Expression Vector

CD711B-1 10 ug
EUR 762

pCDH-MSCV-MCS-IRES-GFP cDNA Cloning and Expression Vector

CD731B-1 10 ug
EUR 783.6

pCDH-MSCV-MCS-EF1-GFP+Puro cDNA Cloning and Expression Vector

CD713B-1 10 ug
EUR 762

pCDH- CMV- MCS- EF1- CopGFP- T2A- Puro

PVT2333 2 ug
EUR 390

pCDH-EF1-MCS-(PGK-GFP-T2A-Puro) Cloning and Expression Vector

CD813A-1 10 ug
EUR 770.4

pCDH-EF1-MCS-T2A-RFP (PGK-Puro) Cloning and Expression Vector

CD822A-1 10 ug
EUR 770.4

pCDH-EF1-MCS-T2A-GFP (PGK-Puro) Cloning and Expression Vector

CD823A-1 10 ug
EUR 770.4

pCDH-CMV-MCS-EF1-copGFP-T2A-Puro plasmid

PVT17271 2 ug
EUR 568.8

pCDH-EF1-MCS-T2A-Puro cDNA Cloning and Expression Vector 10 ug

CD527A-1 10 ug
EUR 783.6

pCDH-CMV-MCS cDNA Cloning and Expression Vector

CD500B-1 10 ug
EUR 652.8

pCDH-EF1-MCS cDNA Cloning and Expression Vector

CD502A-1 10 ug
EUR 652.8

pCDH-EF1-MCS-BGH-PGK-GFP-T2A-Puro cDNA Cloning and Expression Vector

CD550A-1 10 ug
EUR 750

pCDH-EF1-MCS-(PGK-Puro) Cloning and Expression Vector

CD810A-1 10 ug
EUR 770.4

pCDH-EF1-MCS-(PGK-GFP) Cloning and Expression Vector

CD811A-1 10 ug
EUR 770.4

pCDH-EF1-Luc-T2A-copGFP

PVT17649 2 ug
EUR 508.8

PB-MSCV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector

PB713B-1 10 ug
EUR 842.4

pCDH-CMV-MCS-EF1-Puro cDNA Cloning and Expression Vector

CD510B-1 10 ug
EUR 762

pCDH-CMV-MCS-EF1-RFP cDNA Cloning and Expression Vector

CD512B-1 10 ug
EUR 762

pCDH-CMV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector

CD513B-1 10 ug
EUR 762

pCDH-CMV-MCS-EF1-Neo cDNA Cloning and Expression Vector

CD514B-1 10 ug
EUR 762

pCDH-CMV-MCS-EF1-Hygro cDNA Cloning and Expression Vector

CD515B-1 10 ug
EUR 762

pCDH-EF1-MCS-IRES-GFP cDNA Cloning and Expression Vector

CD530A-2 10 ug
EUR 783.6

pCDH-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector

CD531A-2 10 ug
EUR 783.6

pCDH-EF1-MCS-IRES-Puro cDNA Cloning and Expression Vector

CD532A-2 10 ug
EUR 783.6

pCDH-EF1-MCS-IRES-Neo cDNA Cloning and Expression Vector

CD533A-2 10 ug
EUR 783.6

pCDH-UbC-MCS-EF1-Hygro cDNA Cloning and Expression Vector

CD615B-1 10 ug
EUR 762

pCDH-UbC-MCS-IRES-GFP cDNA Cloning and Expression Vector

CD630A-1 10 ug
EUR 783.6

pCDH-EF1-MCS*-T2A-GFP

CD526A-1 10 ug
EUR 783.6

pCDH- CMV- MCS- EF1- copGFP Plasmid

PVT2334 2 ug
EUR 390

pAAVK-EF1? -MCS-T2A-EGFP AAVanced Cloning and Expression Vector

AAV526A-1 10 ug
EUR 812.4

pAAVK-EF1? -MCS-T2A-Puro AAVanced Cloning and Expression Vector

AAV527A-1 10 ug
EUR 812.4

pAAVK-EF1? ?MCS-T2A-mRFP AAVanced Cloning and Expression Vector

AAV528A-1 10 ug
EUR 812.4

pCDH-CMV-MCS-EF1-RFP+Puro cDNA Cloning and Expression Vector

CD516B-2 10 ug
EUR 762

pCDH- EF1- MCS- T2A- Puro Plasmid

PVT2335 2 ug
EUR 390

XStamp cloning and expression lentivector MSCV-Leader-MCS-C1C2-EF1-Puro

XSTP710PA-1 10 ug
EUR 956.4

pCDH-CMV-Akaluc-EF1-CopGFP-T2A-Puro

PVT14490 2 ug
EUR 718.8

pCDH-CuO-MCS-T2A-GFP co-inducible T2A lentivector

QM521A-1 10 ug
EUR 814.8

pCDH-CuO-MCS-T2A-RFP co-inducible T2A lentivector

QM522A-1 10 ug
EUR 814.8

pSIH1-H1-copGFP shRNA Cloning and Expression Vector

SI501A-1 10 ug
EUR 680.4

pCDH-Cuo-MCS-EF1-CymR-T2A-Puro

QM800A-1 10 ug
EUR 1572

pCDH- CMV- MCS- EF1- turboRFP- T2A- Puro

PVT11077 2 ug
EUR 444

pCDH-EF1-MCS-T2A-Puro (CD520A) plasmid

PVT17273 2 ug
EUR 568.8

pCDH-CMV-efemp1-m-EF1-CopGFP-T2A-Puro

PVT17438 2 ug
EUR 360

pCDH-CMV-TXNL1-HA-EF1-CopGFP-T2A-Puro

PVT17693 2 ug
EUR 309.6

pCDH-CMV-CCDC68-HA-EF1-CopGFP-T2A-Puro

PVT17760 2 ug
EUR 309.6

pCDH-CMV-ccl3-m-EF1-CopGFP-T2A-Puro

PVT19054 2 ug
EUR 360

pCDH-CMV-PTX3-EF1-CopGFP-T2A-Puro Plasmid

PVT17116 2 ug
EUR 390

pCDH-CMV-MCS2 cDNA Cloning and Expression Vector

CD501A-1 10 ug
EUR 652.8

pAAVK-EF1? -MCS AAVanced Cloning and Expression Vector

AAV502A-1 10 ug
EUR 812.4

pCDF1-MCS2-EF1-copGFP cDNA Cloning and Expression Vector

CD111B-1 10 ug
EUR 762

pCDH-CuO-MCS empty cloning lentivector

QM500A-1 10 ug
EUR 716.4

BLIV 2.0 Reporter: MSCV-Luciferase-EF1a-copGFP-T2A-Puro Lentivector Plasmid

BLIV713PA-1 10 ug
EUR 963.6

pCDH-CMV-MCS-EF1-copGFP cDNA Clon/Exp Vector [pre-packaged virus]

CD511VB-1 >1 x 10^6 IFUs
EUR 943.2

pCDH-CMV-MCS-EF1-copGFP+Puro cDNA Clon/Exp Vector [pre-packaged]

CD513VB-1 >1 x 10^6 IFUs
EUR 943.2

Scramble control hairpin in pCDH-CMV-MCS-EF1-copGFP (CD511B-1)

PMIRH000PA-1 Bacterial Streak
EUR 787.2

PB-CMV-MCS-EF1-Puro cDNA cloning and expression vector

PB510B-1 10 ug
EUR 842.4

PB-CMV-MCS-EF1-GFP cDNA cloning and expression vector

PB511B-1 10 ug
EUR 842.4

PB-CMV-MCS-EF1-RFP cDNA cloning and expression vector

PB512B-1 10 ug
EUR 842.4

PB-CMV-MCS-EF1-GreenPuro cDNA cloning and expression vector

PB513B-1 10 ug
EUR 842.4

PB-CMV-MCS-EF1-RedPuro cDNA Cloning and Expression Vector

PB514B-2 10 ug
EUR 842.4

PB-EF1-MCS-IRES-GFP cDNA cloning and expression vector

PB530A-2 10 ug
EUR 842.4

PB-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector

PB531A-2 10 ug
EUR 842.4

PB-EF1-MCS-IRES-Neo cDNA cloning and expression vector

PB533A-2 10 ug
EUR 842.4

PB-CMV-GreenPuro-H1-MCS shRNA cloning and expression vector

PBSI505A-1 10 ug
EUR 842.4

PB-EF1-GreenPuro-H1-MCS shRNA cloning and expression vector

PBSI506A-1 10 ug
EUR 842.4

XPack MSCV-XP-MCS-EF1-Puro Cloning Lentivector

XPAK710PA-1 10 ug
EUR 956.4

BLIV 2.0 Reporter: MSCV-Luciferase-EF1a-copGFP-T2A-Puro Pre-packaged Virus

BLIV713VA-1 >2 x10^6 IFUs
EUR 963.6

pAAV-MCS Expression Vector

VPK-410 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pscAAV-MCS Expression Vector

VPK-430 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pCDH- CMV- mCherry- EF1- CopGFP- Puro

PVT11078 2 ug
EUR 444

pCDH-CMV-GSTA1-EF1-copGFP Plasmid

PVT16093 2 ug
EUR 390

pCDH- CMV- MCS- EF1- puro

PVT11075 2 ug
EUR 444

pCDH- CMV- MCS- EF1- Hygro

PVT11076 2 ug
EUR 444

T2A-GFP Co-Expression HR Targeting Vector [T2A-GFP-pA-loxP-EF1?-RFP-T2A-Puro-pA-LoxP-MCS]

HR130PA-1 10 ug
EUR 1227.6

pCDH-EF1-Luc2-T2A-ZsGreen1

PVT17917 2 ug
EUR 508.8

pCDH-CMV-MCS-EF1-copGFP cDNA Clon/Exp Vector [pre-packaged Ultra-High Titer virus]

CD511VB-UHT >1x10^9 IFUs
EUR 1249.2

pCDH-CMV-exosome booster-EF1-copGFP-Puro vector

PVT12109 2 ug
EUR 1149.6

pAAV-MCS Promoterless Expression Vector

VPK-411 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pMC.CMV-GFP-T2A-Puro-H1-MCS (shRNA expression parental minicircle plasmid)

MNSI505A-1 10 ug
EUR 1016.4

pMC.EF1-GFP-T2A-Puro-H1-MCS (shRNA expression parental minicircle plasmid)

MNSI506A-1 10 ug
EUR 1016.4

GFP-T2A-Luciferase Co-Expression HR Targeting Vector [GFP-T2A-Luc-pA-loxP-EF1?-RFP-T2A-Puro-pA-LoxP-MCS]

HR150PA-1 10 ug
EUR 1227.6

pCDH-CMV-MCS-EF1-EGFP-IRFP

PVT17586 2 ug
EUR 508.8

pCDH- ECMV- MCS- 3*Flag- EF1

PVT11080 2 ug
EUR 444

pCDH-CMV-MCS-EF1a-GFP+BSD

PVT17282 2 ug
EUR 469.2

pCDH-EF1-CymR-T2A-Puro (plasmid)

QM200PA-2 10 ug
EUR 716.4

pCDH-EF1-CymR-T2A-Puro (virus)

QM200VA-2 >1 x 10^6 IFUs
EUR 763.2

pCDH-EF1-CymR-T2A-RFP (plasmid)

QM300PA-1 10 ug
EUR 716.4

pCDH-EF1-CymR-T2A-RFP (virus)

QM300VA-1 >1 x 10^6 IFUs
EUR 763.2

pCDH-EF1-CymR-T2A-Neo (plasmid)

QM400PA-2 10 ug
EUR 697.2

pCDH-EF1-CymR-T2A-Neo (virus)

QM400VA-2 >1 x 10^6 IFUs
EUR 744

pCDH- EF1- luc2- T2A- Tdtomato Plasmid

PVT2337 2 ug
EUR 289.2

pCDH-NFAT-Luc2-T2A-tdTomato Plasmid

PVT16080 2 ug
EUR 390

PB-Cuo-MCS-IRES-GFP-EF1-CymR-Puro Inducible cDNA Cloning and Expression Vector

PBQM812A-1 10 ug
EUR 1315.2

pGreen- Puro- pSIH1- H1- copGFP- T2A- Puro

PVT11116 2 ug
EUR 444

MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid

CASLV120PA-1 10 ug
EUR 836.4

pCDF1-MCS1 cDNA Cloning and Expression Vector

CD100A-1 10 ug
EUR 652.8

pCDH-CMV-MCS-EF1-Puro (CD510B) plasmid

PVT17272 2 ug
EUR 568.8

pCDH-CuO-MCS-IRES-GFP-EF1-CymR-T2A-Puro All-in-one inducible IRES lentivector

QM812B-1 10 ug
EUR 1572

pCDH-CMV-GLUT1-EF1-turboRFP-T2A-Puro

PVT13588 2 ug
EUR 718.8

pCDH-ME-APOE-Luc2-T2A-tdTomato Plasmid

PVT17253 2 ug
EUR 444

pCDH-CuO-MCS-EF1-GFP+Puro dual promoter and dual marker lentivector

QM513B-1 10 ug
EUR 814.8

pCDH-CuO-MCS-EF1-RFP+Puro dual promoter and dual marker lentivector

QM516B-2 10 ug
EUR 814.8

Constitutive (CAGs promoter) EEV cloning and expression vector

EEV600A-1 10 ug
EUR 812.4

Cumate-Inducible (CuO) EEV cloning and expression vector

EEV610A-1 10 ug
EUR 812.4

pSIH1-H1-Puro shRNA Cloning and Expression Vector

SI500A-1 10 ug
EUR 680.4

pSIH1-H1-H2Kk shRNA Cloning and Expression Vector

SI502A-1 10 ug
EUR 680.4

pCDH-CuO-MCS-EF1-GFP dual promoter lentivector

QM511B-1 10 ug
EUR 814.8

pCDH-CuO-MCS-EF1-RFP dual promoter lentivector

QM512B-1 10 ug
EUR 814.8

pCDH-CMV-MCS-EF1-Puro-FLAG-HIF2 alpha

PVT12166 2 ug
EUR 444

pCDH-Cuo-RFP-T2A-GFP (positive control plasmid)

QM350PA-1 10 ug
EUR 716.4

pCDH-Cuo-RFP-T2A-GFP (positive control virus)

QM350VA-1 >1 x 10^6 IFUs
EUR 763.2

pCDH-CuO-MCS-IRES-GFP co-inducible IRES lentivector

QM530A-2 10 ug
EUR 814.8

pCDH-CuO-MCS-IRES-RFP co-inducible IRES lentivector

QM531A-2 10 ug
EUR 814.8

pCDH-CMV-MCS-EF1-aRFP cDNA Clon/Exp Vector [pre-packaged]

CD512VA-1 >1 x 10^6 IFUs
EUR 943.2

pCDH-EF1a-Piranha-T2A-Puro virus (>1x10^6 IFUs)

PTPD527VA-1 >1x10^6 IFUs
EUR 836.4

XPack MSCV-XP-GFP-EF1-Puro Expression Lentivector

XPAK730PA-1 10 ug
EUR 991.2

XPack MSCV-XP-GFP-EF1-Puro Expression Lentivirus

XPAK730VA-1 >1 x 10^6 IFUs
EUR 991.2

XPack MSCV-XP-Luciferase-EF1-Puro Expression Lentivector

XPAK732PA-1 10 ug
EUR 991.2

XPack MSCV-XP-Luciferase-EF1-Puro Expression Lentivirus

XPAK732VA-1 >1 x 10^6 IFUs
EUR 991.2

MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV120PA-KIT 1 Kit
EUR 1358.4

Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid

CASLV220PA-1 10 ug
EUR 836.4

Minicircle Dual Reporter: MSCV-RFP-T2A-Luciferase DNA (30 ug)

BLIV506MC-1 30 ug
EUR 859.2

Minicircle Dual Reporter: MSCV-GFP-T2A-Luciferase DNA (30 ug)

BLIV507MC-1 30 ug
EUR 859.2

pFC-EF1-MCS-pA-PGK-RFP-T2A-Puro PhiC31 Donor Vector

FC550A-1 10ug
EUR 927.6

pFC-PGK-MCS-pA-EF1-GFP-T2A-Puro PhiC31 Donor Vector

FC551A-1 10ug
EUR 927.6

pAAVK-EF1?-MCS1-CMV-MCS2 AAVanced Cloning and Expression Vector

AAV503A-1 10 ug
EUR 812.4

pAAVK-EF1?-MCS1-CMV-EGFP AAVanced Cloning and Expression Vector

AAV536A-1 10 ug
EUR 812.4

pAAVK-EF1?-MCS1-CMV-Puro AAVanced Cloning and Expression Vector

AAV537A-1 10 ug
EUR 812.4

pAAVK-EF1?-MCS1-CMV-mRFP AAVanced Cloning and Expression Vector

AAV538A-1 10 ug
EUR 812.4

pCDH-CMV-MCS-EF1-RFP+Puro cDNA Clon/Exp Vector [pre-packaged]

CD516VB-2 >1 x 10^6 IFUs
EUR 926.4

Minicircle Dual Reporter: MSCV-RFP-T2A-Luciferase Parental Plasmid (10 ug)

BLIV506MN-1 10 ug
EUR 1166.4

Minicircle Dual Reporter: MSCV-GFP-T2A-Luciferase Parental Plasmid (10 ug)

BLIV507MN-1 10 ug
EUR 1166.4

pCDF1-MCS2-EF1-Puro cDNA Cloning and Expression Lentivector

CD110B-1 10 ug
EUR 762

pCDH-CMV-MCS-EF1-Puro cDNA Vector cDNA Clon/Exp Vector [pre-packaged virus]

CD510VB-1 >1 x 10^6 IFUs
EUR 943.2

PCDH

EF007042 96 Tests
EUR 826.8

PCDH

EF007043 96 Tests
EUR 826.8

pCDH-CMV-Piranha-EF1-GFP-T2A-Puro virus (>1x10^6 IFUs)

PTPD513VA-1 >1x10^6 IFUs
EUR 836.4

MSCV-hspCas9-T2A-Puro SmartNuclease Pre-Packaged Lentiviral Particles [>10^6 IFUs]

CASLV120VA-1 2 x 25ul
EUR 836.4

pMC.CMV-GFP-H1-MCS (shRNA expression parental minicircle plasmid)

MNSI500A-1 10 ug
EUR 1016.4

Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV220PA-KIT 1 Kit
EUR 1358.4

MSCV-hspCas9-T2A-Puro-H1-gRNA linearized all-in-one SmartNuclease Lentivector Plasmid

CASLV320PA-1 10 rxn
EUR 966

MSCV-Mouse Foxp3-EF1-GFP-T2A-Puro Stable Jurkat cell line (Puro resistant)

TCL110C-1 5 x 10^6 cells/ml
EUR 2018.4

MSCV-Human Foxp3-EF1-GFP-T2A-Puro Stable Jurkat cell line (Puro resistant)

TCL210C-1 5 x 10^6 cells/ml
EUR 2018.4

MSCV-Mouse RORgt-EF1-GFP-T2A-Puro Stable Jurkat cell line (Puro resistant)

TCL310C-1 5 x 10^6 cells/ml
EUR 2018.4

MSCV-Human RORgt-EF1-GFP-T2A-Puro Stable Jurkat cell line (Puro resistant)

TCL410C-1 5 x 10^6 cells/ml
EUR 2018.4

XPack CMV-XP-MCS-EF1-Puro Cloning Lentivector

XPAK510PA-1 10 ug
EUR 956.4

MSCV JMJD3 Plasmid

PVT17098 2 ug
EUR 390

GFP-Fusion HR Targeting Vector [GFP-pA-LoxP-EF1?-RFP-T2A-Puro-pA-LoxP-MCS]

HR120PA-1 10 ug
EUR 1227.6

GFP-Fusion HR Targeting Vector [GFP-pA-LoxP-EF1a-RFP-T2A-Hygro-pA-LoxP-MCS]

HR220PA-1 10 ug
EUR 1227.6

PB-Cuo-shMCS-IRES-GFP-EF1-CymR-Puro Inducible shRNA Cloning and Expression Vector

PBQMSH812A-1 10 ug
EUR 1315.2

pGEN- MCS

PVT10565 2 ug
EUR 444

pLEX- MCS

PVT11092 2 ug
EUR 361.2

PinPoint-HR attP Placement Vector ? Empty MCS for cloning HR Arms

PIN400A-1 10ug
EUR 1521.6

Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Pre-Packaged Lentiviral Particles [>10^6 IFUs]

CASLV220VA-1 2 x 25ul
EUR 836.4

MSCV-hspCas9-H1-gRNA SmartNuclease vector

CAS960A-1 10 ug
EUR 966

pCDH- CMV- Rnd2

PVT10402 2 ug
EUR 361.2

pCDH- CMV- IDH1

PVT10169 2 ug
EUR 361.2

pCDH- CMV- CAMK2G

PVT10240 2 ug
EUR 361.2

MCS ORF Vector (Human) (pORF)

ORF023399 1.0 ug DNA Ask for price

Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro-H1-gRNA linearized all-in-one SmartNuclease Lentivector Plasmid

CASLV420PA-1 10 rxn
EUR 966

pMC.CMV-MCS-SV40polyA

MN501A-1 10 ug
EUR 882

pMC.EF1-MCS-SV40polyA

MN502A-1 10 ug
EUR 882

pEnCMV-MCS-3

PVT17410 2 ug
EUR 360

pMyosin-MCS-PSmOrange

PVT17668 2 ug
EUR 459.6

pECMV-MCS-Myc

PVT17857 2 ug
EUR 409.2

pLEGFP-MCS-FLAG

PVT18164 2 ug
EUR 409.2

pAAV- MCS Plasmid

PVT2102 2 ug
EUR 289.2

pT7- His- MCS

PVT10595 2 ug
EUR 319.2

pEU- E01- MCS

PVT11379 2 ug
EUR 444

pAAVS1-P-MCS

PVT12296 2 ug
EUR 843.6

pMD-AOX-MCS

PVT14289 2 ug
EUR 1093.2

pCDNA3.1-MCS-6

PVT15973 2 ug
EUR 390

E. coli gyrase ATPase assay Kit Plus (enzyme included)

T2A-100KE 100 assays
EUR 444.91
Description: This product includes the reaction buffer, DNA, E. coli gyrase, ATP and the phosphate detection reagent for 100 assays of DNA supercoiling reactions in a 384-well assay format.

P. aeruginosa Gyrase ATPase assay Kit Plus (enzyme included)

T2A-100KP 100 assays
EUR 444.91
Description: This product includes the reaction buffer, DNA, P. aeruginosa gyrase, ATP and the phosphate detection reagent for 100 assays of DNA supercoiling reactions in a 384-well assay format.

S. aureus gyrase ATPase assay Kit Plus (enzyme included)

T2A-100KS 100 assays
EUR 423.72
Description: This product includes the reaction buffer, DNA, S. aureus gyrase, ATP and the phosphate detection reagent for 100 assays of DNA supercoiling reactions in a 384-well assay format.

pPS-MSCV-GFP-RFP (plasmid)

LV605PA-1 10 ug
EUR 787.2

PCDH-X/Y Antibody

DF8867 200ul
EUR 420

PCDH-X/Y Antibody

45549-100ul 100ul
EUR 302.4

PCDH-X/Y Antibody

45549-50ul 50ul
EUR 224.4

PCDH- X/Y Antibody

ABD8867 100 ug
EUR 525.6

PCDH-X / Y Antibody

20-abx218014
  • EUR 510.00
  • EUR 410.40
  • 100 ug
  • 50 ug

pCDH-513B-CTH Plasmid

PVTB00700-4c 2 ug
EUR 427.2

pCDH-puro-CSF1R Plasmid

PVTB00726-4a 2 ug
EUR 427.2

pCDH- puro- Bcl- XL

PVT10290 2 ug
EUR 361.2

pYLEX1 - Expression Vector

FYY203-5MG 5mg Ask for price

pORB-MCS-sIRES-VSVG

ABP-BM-sIRESVG 5 ug Ask for price

pCS2-EGFP-MCS-3

PVT17867 2 ug
EUR 360


CONCLUSION
Recombinant eukaryotic expression vector pCDH-FAP successfully constructed. These results provide a basis for further study on the function of FAP in vitro.

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