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Neuronal differentiation of hair-follicle-bulge-derived stem cells co-cultured with mouse cochlear modiolus explants.
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Stem cell-based repair auditory neurons may represent an attractive therapeutic option to restore sensorineural hearing loss. stem cells of hair-follicle-bulge-derived (HFBSCs) are promising candidates for this type of therapy, because they (1) have the nature of migration, allowing migration after transplantation, (2) can differentiate into sensory neurons and glial cells, and (3) can easily be harvested in relatively high amounts. However, HFBSCs never used for this purpose.
We hypothesize that HFBSCs can be used to repair the auditory nerve cell-based and we have researched their migration and incorporation into the cochlear modiolus explant and subsequent differentiation of them. Modiolus explants derived from adult wild type mice were cultured in the presence of HFBSCs EF1α-copGFP-transduced, constitutively expressing green fluorescent protein copepods (copGFP). Also, explants modiolus without hair cells were co-cultured with DCX-copGFP HFBSCs-transduced, indicating copGFP on doublecortin expression during neuronal differentiation.
HFBSC speed migration towards modiolus explants is calculated, and after two weeks, co-cultures were fixed and processed for immunohistochemical staining. EF1α-copGFP HFBSC rapid migration speed: 80.5 ± 6.1 m / h. After arriving in explants, cells form a pattern fasciculus and change their phenotype into neural cell types Atoh1-positive. DCX-copGFP HFBSCs green-fluorescent after integration into the explants, justify the differentiation of neurons from cells. These results indicate that HFBSC derived progenitor neuronal migration and can integrate into the cochlear modiolus explants, while adapting their phenotypes depending on the micro environment. Thus, HFBSCs demonstrate the potential to be employed in cell-based therapies to repair the auditory nerve.
The immunomodulatory and self-renewable human features adipose-derived mesenchymal stem cells (HAD-MSCs) mark their importance in regenerative medicine. Interleukin (IL) -23 as proinflammatory cytokines Hitting the regulatory T cells and promote the response of T helper 17 and T helper 1 cells. This initiation pathways of inflammation and immunosuppression in several autoimmune diseases.
[Construction of eukaryotic expression vector fibroblast activation protein and the formation of stable over-expression in squamous cell carcinoma of the mouth].
OBJECTIVE
This study aimed to construct vectors fibroblast activation protein (FAP) over-expression lentivinus to investigate transfection in cell lines and build a stable FAP SCC9 over-expression of oral squamous cell lines.
METHOD
The cDNA of the FAP gene from squamous cell carcinoma of the oral tissues (OSCC) was amplified by polymerase chain reaction (PCR) and subkloning into eukaryotic expression vector pCDH-CMV-MCS-EF1-copGFP. Recombinant plasmids were lined up and then transfected into cell lines SCC9. Further, cell lines which over-expressed FAP SCC9 stable founded by fluorescence activated cell sorting (FACS). Expression of green fluorescent protein (GFP) was detected with a fluorescence microscope, and over-expression of FAP identified by real-time PCR and Western blot.
RESULTS
FAP gene amplified by PCR and then cloned into a vector, whose sequence is identical to that in GenBank. GFP was expressed in transfected cells. Furthermore, FAP over-expression in transfected cells was detected by real-time PCR and Western blot.
 pCDH-CMV-MCS-t2A-copGFP cDNA Cloning and Expression Vector |
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| CD524A-1 | SBI | 10 ug | EUR 653 |
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 pCDH-MCS-T2A-Puro-MSCV cDNA Cloning and Expression Vector |
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| CD522A-1 | SBI | 10 ug | EUR 653 |
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 pCDH- EF1- MCS- T2A- copGFP |
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| PVT2336 | Lifescience Market | 2 ug | EUR 325 |
 pCDH-CMV-MCS-EF1-copGFP cDNA Cloning and Expression Vector |
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| CD511B-1 | SBI | 10 ug | EUR 635 |
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 pCDH-MSCV-MCS-EF1-Puro cDNA Cloning and Expression Vector |
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| CD710B-1 | SBI | 10 ug | EUR 635 |
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 pCDH-MSCV-MCS-EF1-GFP cDNA Cloning and Expression Vector |
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| CD711B-1 | SBI | 10 ug | EUR 635 |
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 pCDH-MSCV-MCS-IRES-GFP cDNA Cloning and Expression Vector |
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| CD731B-1 | SBI | 10 ug | EUR 653 |
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 pCDH-MSCV-MCS-EF1-GFP+Puro cDNA Cloning and Expression Vector |
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| CD713B-1 | SBI | 10 ug | EUR 635 |
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 pCDH- CMV- MCS- EF1- CopGFP- T2A- Puro |
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| PVT2333 | Lifescience Market | 2 ug | EUR 325 |
 Cloning and Expression Vector) pCDH-EF1-MCS-(PGK-GFP-T2A-Puro) Cloning and Expression Vector |
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| CD813A-1 | SBI | 10 ug | EUR 642 |
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 Cloning and Expression Vector) pCDH-EF1-MCS-T2A-RFP (PGK-Puro) Cloning and Expression Vector |
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| CD822A-1 | SBI | 10 ug | EUR 642 |
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 Cloning and Expression Vector) pCDH-EF1-MCS-T2A-GFP (PGK-Puro) Cloning and Expression Vector |
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| CD823A-1 | SBI | 10 ug | EUR 642 |
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 pCDH-CMV-MCS-EF1-copGFP-T2A-Puro plasmid |
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| PVT17271 | Lifescience Market | 2 ug | EUR 474 |
 pCDH-EF1-MCS-T2A-Puro cDNA Cloning and Expression Vector 10 ug |
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| CD527A-1 | SBI | 10 ug | EUR 653 |
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 pCDH-CMV-MCS cDNA Cloning and Expression Vector |
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| CD500B-1 | SBI | 10 ug | EUR 544 |
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 pCDH-EF1-MCS cDNA Cloning and Expression Vector |
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| CD502A-1 | SBI | 10 ug | EUR 544 |
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 pCDH-EF1-MCS-BGH-PGK-GFP-T2A-Puro cDNA Cloning and Expression Vector |
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| CD550A-1 | SBI | 10 ug | EUR 625 |
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 Cloning and Expression Vector) pCDH-EF1-MCS-(PGK-Puro) Cloning and Expression Vector |
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| CD810A-1 | SBI | 10 ug | EUR 642 |
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 Cloning and Expression Vector) pCDH-EF1-MCS-(PGK-GFP) Cloning and Expression Vector |
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| CD811A-1 | SBI | 10 ug | EUR 642 |
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 pCDH-EF1-Luc-T2A-copGFP |
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| PVT17649 | Lifescience Market | 2 ug | EUR 424 |
 PB-MSCV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector |
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| PB713B-1 | SBI | 10 ug | EUR 702 |
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 pCDH-CMV-MCS-EF1-Puro cDNA Cloning and Expression Vector |
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| CD510B-1 | SBI | 10 ug | EUR 635 |
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 pCDH-CMV-MCS-EF1-RFP cDNA Cloning and Expression Vector |
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| CD512B-1 | SBI | 10 ug | EUR 635 |
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 pCDH-CMV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector |
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| CD513B-1 | SBI | 10 ug | EUR 635 |
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 pCDH-CMV-MCS-EF1-Neo cDNA Cloning and Expression Vector |
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| CD514B-1 | SBI | 10 ug | EUR 635 |
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 pCDH-CMV-MCS-EF1-Hygro cDNA Cloning and Expression Vector |
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| CD515B-1 | SBI | 10 ug | EUR 635 |
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 pCDH-EF1-MCS-IRES-GFP cDNA Cloning and Expression Vector |
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| CD530A-2 | SBI | 10 ug | EUR 653 |
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 pCDH-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector |
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| CD531A-2 | SBI | 10 ug | EUR 653 |
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 pCDH-EF1-MCS-IRES-Puro cDNA Cloning and Expression Vector |
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| CD532A-2 | SBI | 10 ug | EUR 653 |
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 pCDH-EF1-MCS-IRES-Neo cDNA Cloning and Expression Vector |
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| CD533A-2 | SBI | 10 ug | EUR 653 |
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 pCDH-UbC-MCS-EF1-Hygro cDNA Cloning and Expression Vector |
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| CD615B-1 | SBI | 10 ug | EUR 635 |
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 pCDH-UbC-MCS-IRES-GFP cDNA Cloning and Expression Vector |
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| CD630A-1 | SBI | 10 ug | EUR 653 |
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 pCDH-EF1-MCS*-T2A-GFP |
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| CD526A-1 | SBI | 10 ug | EUR 653 |
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 pCDH- CMV- MCS- EF1- copGFP Plasmid |
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| PVT2334 | Lifescience Market | 2 ug | EUR 325 |
 pAAVK-EF1? -MCS-T2A-EGFP AAVanced Cloning and Expression Vector |
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| AAV526A-1 | SBI | 10 ug | EUR 677 |
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 pAAVK-EF1? -MCS-T2A-Puro AAVanced Cloning and Expression Vector |
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| AAV527A-1 | SBI | 10 ug | EUR 677 |
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 pAAVK-EF1? ?MCS-T2A-mRFP AAVanced Cloning and Expression Vector |
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| AAV528A-1 | SBI | 10 ug | EUR 677 |
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 pCDH-CMV-MCS-EF1-RFP+Puro cDNA Cloning and Expression Vector |
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| CD516B-2 | SBI | 10 ug | EUR 635 |
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 XStamp cloning and expression lentivector MSCV-Leader-MCS-C1C2-EF1-Puro |
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| XSTP710PA-1 | SBI | 10 ug | EUR 797 |
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 pCDH- EF1- MCS- T2A- Puro Plasmid |
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| PVT2335 | Lifescience Market | 2 ug | EUR 325 |
 pCDH-CMV-Akaluc-EF1-CopGFP-T2A-Puro |
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| PVT14490 | Lifescience Market | 2 ug | EUR 599 |
 pCDH-CuO-MCS-T2A-GFP co-inducible T2A lentivector |
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| QM521A-1 | SBI | 10 ug | EUR 679 |
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 pCDH-CuO-MCS-T2A-RFP co-inducible T2A lentivector |
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| QM522A-1 | SBI | 10 ug | EUR 679 |
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 pCDH-Cuo-MCS-EF1-CymR-T2A-Puro |
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| QM800A-1 | SBI | 10 ug | EUR 1310 |
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 pCDH- CMV- MCS- EF1- turboRFP- T2A- Puro |
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| PVT11077 | Lifescience Market | 2 ug | EUR 370 |
 plasmid) pCDH-EF1-MCS-T2A-Puro (CD520A) plasmid |
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| PVT17273 | Lifescience Market | 2 ug | EUR 474 |
 pSIH1-H1-copGFP shRNA Cloning and Expression Vector |
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| SI501A-1 | SBI | 10 ug | EUR 567 |
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 pCDH-CMV-TXNL1-HA-EF1-CopGFP-T2A-Puro |
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| PVT17693 | Lifescience Market | 2 ug | EUR 258 |
 pCDH-CMV-CCDC68-HA-EF1-CopGFP-T2A-Puro |
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| PVT17760 | Lifescience Market | 2 ug | EUR 258 |
 pCDH-CMV-ccl3-m-EF1-CopGFP-T2A-Puro |
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| PVT19054 | Lifescience Market | 2 ug | EUR 300 |
 pCDH-CMV-PTX3-EF1-CopGFP-T2A-Puro Plasmid |
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| PVT17116 | Lifescience Market | 2 ug | EUR 325 |
 pCDH-CMV-efemp1-m-EF1-CopGFP-T2A-Puro |
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| PVT17438 | Lifescience Market | 2 ug | EUR 300 |
 pCDH-CMV-MCS2 cDNA Cloning and Expression Vector |
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| CD501A-1 | SBI | 10 ug | EUR 544 |
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 pAAVK-EF1? -MCS AAVanced Cloning and Expression Vector |
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| AAV502A-1 | SBI | 10 ug | EUR 677 |
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 pCDF1-MCS2-EF1-copGFP cDNA Cloning and Expression Vector |
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| CD111B-1 | SBI | 10 ug | EUR 635 |
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 pCDH-CuO-MCS empty cloning lentivector |
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| QM500A-1 | SBI | 10 ug | EUR 597 |
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 BLIV 2.0 Reporter: MSCV-Luciferase-EF1a-copGFP-T2A-Puro Lentivector Plasmid |
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| BLIV713PA-1 | SBI | 10 ug | EUR 803 |
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 pCDH-CMV-MCS-EF1-copGFP cDNA Clon/Exp Vector [pre-packaged virus] |
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| CD511VB-1 | SBI | >1 x 10^6 IFUs | EUR 786 |
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 pCDH-CMV-MCS-EF1-copGFP+Puro cDNA Clon/Exp Vector [pre-packaged] |
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| CD513VB-1 | SBI | >1 x 10^6 IFUs | EUR 786 |
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) Scramble control hairpin in pCDH-CMV-MCS-EF1-copGFP (CD511B-1) |
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| PMIRH000PA-1 | SBI | Bacterial Streak | EUR 656 |
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 PB-CMV-GreenPuro-H1-MCS shRNA cloning and expression vector |
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| PBSI505A-1 | SBI | 10 ug | EUR 702 |
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 PB-EF1-GreenPuro-H1-MCS shRNA cloning and expression vector |
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| PBSI506A-1 | SBI | 10 ug | EUR 702 |
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 PB-CMV-MCS-EF1-Puro cDNA cloning and expression vector |
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| PB510B-1 | SBI | 10 ug | EUR 702 |
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 PB-CMV-MCS-EF1-GFP cDNA cloning and expression vector |
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| PB511B-1 | SBI | 10 ug | EUR 702 |
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 PB-CMV-MCS-EF1-RFP cDNA cloning and expression vector |
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| PB512B-1 | SBI | 10 ug | EUR 702 |
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 PB-CMV-MCS-EF1-GreenPuro cDNA cloning and expression vector |
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| PB513B-1 | SBI | 10 ug | EUR 702 |
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 PB-CMV-MCS-EF1-RedPuro cDNA Cloning and Expression Vector |
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| PB514B-2 | SBI | 10 ug | EUR 702 |
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 PB-EF1-MCS-IRES-GFP cDNA cloning and expression vector |
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| PB530A-2 | SBI | 10 ug | EUR 702 |
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 PB-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector |
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| PB531A-2 | SBI | 10 ug | EUR 702 |
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 PB-EF1-MCS-IRES-Neo cDNA cloning and expression vector |
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| PB533A-2 | SBI | 10 ug | EUR 702 |
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 XPack MSCV-XP-MCS-EF1-Puro Cloning Lentivector |
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| XPAK710PA-1 | SBI | 10 ug | EUR 797 |
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 BLIV 2.0 Reporter: MSCV-Luciferase-EF1a-copGFP-T2A-Puro Pre-packaged Virus |
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| BLIV713VA-1 | SBI | >2 x10^6 IFUs | EUR 803 |
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 pAAV-MCS Expression Vector |
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| VPK-410 | Cell Biolabs | 10 µg | EUR 647 |
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Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV. |
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 pscAAV-MCS Expression Vector |
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| VPK-430 | Cell Biolabs | 10 µg | EUR 647 |
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Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV. |
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 pCDH- CMV- mCherry- EF1- CopGFP- Puro |
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| PVT11078 | Lifescience Market | 2 ug | EUR 370 |
 pCDH-CMV-GSTA1-EF1-copGFP Plasmid |
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| PVT16093 | Lifescience Market | 2 ug | EUR 325 |
 T2A-GFP Co-Expression HR Targeting Vector [T2A-GFP-pA-loxP-EF1?-RFP-T2A-Puro-pA-LoxP-MCS] |
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| HR130PA-1 | SBI | 10 ug | EUR 1023 |
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 pCDH- CMV- MCS- EF1- puro |
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| PVT11075 | Lifescience Market | 2 ug | EUR 370 |
 pCDH- CMV- MCS- EF1- Hygro |
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| PVT11076 | Lifescience Market | 2 ug | EUR 370 |
 pCDH-EF1-Luc2-T2A-ZsGreen1 |
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| PVT17917 | Lifescience Market | 2 ug | EUR 424 |
 pCDH-CMV-MCS-EF1-copGFP cDNA Clon/Exp Vector [pre-packaged Ultra-High Titer virus] |
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| CD511VB-UHT | SBI | >1x10^9 IFUs | EUR 1041 |
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 pCDH-CMV-exosome booster-EF1-copGFP-Puro vector |
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| PVT12109 | Lifescience Market | 2 ug | EUR 958 |
 pAAV-MCS Promoterless Expression Vector |
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| VPK-411 | Cell Biolabs | 10 µg | EUR 647 |
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Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV. |
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) pMC.CMV-GFP-T2A-Puro-H1-MCS (shRNA expression parental minicircle plasmid) |
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| MNSI505A-1 | SBI | 10 ug | EUR 847 |
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) pMC.EF1-GFP-T2A-Puro-H1-MCS (shRNA expression parental minicircle plasmid) |
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| MNSI506A-1 | SBI | 10 ug | EUR 847 |
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 GFP-T2A-Luciferase Co-Expression HR Targeting Vector [GFP-T2A-Luc-pA-loxP-EF1?-RFP-T2A-Puro-pA-LoxP-MCS] |
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| HR150PA-1 | SBI | 10 ug | EUR 1023 |
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 pCDH- ECMV- MCS- 3*Flag- EF1 |
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| PVT11080 | Lifescience Market | 2 ug | EUR 370 |
 pCDH-CMV-MCS-EF1a-GFP+BSD |
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| PVT17282 | Lifescience Market | 2 ug | EUR 391 |
 pCDH-CMV-MCS-EF1-EGFP-IRFP |
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| PVT17586 | Lifescience Market | 2 ug | EUR 424 |
 pCDH- EF1- luc2- T2A- Tdtomato Plasmid |
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| PVT2337 | Lifescience Market | 2 ug | EUR 241 |
) pCDH-EF1-CymR-T2A-Puro (plasmid) |
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| QM200PA-2 | SBI | 10 ug | EUR 597 |
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) pCDH-EF1-CymR-T2A-Puro (virus) |
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| QM200VA-2 | SBI | >1 x 10^6 IFUs | EUR 636 |
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) pCDH-EF1-CymR-T2A-RFP (plasmid) |
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| QM300PA-1 | SBI | 10 ug | EUR 597 |
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) pCDH-EF1-CymR-T2A-RFP (virus) |
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| QM300VA-1 | SBI | >1 x 10^6 IFUs | EUR 636 |
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) pCDH-EF1-CymR-T2A-Neo (plasmid) |
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| QM400PA-2 | SBI | 10 ug | EUR 581 |
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) pCDH-EF1-CymR-T2A-Neo (virus) |
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| QM400VA-2 | SBI | >1 x 10^6 IFUs | EUR 620 |
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 pCDH-NFAT-Luc2-T2A-tdTomato Plasmid |
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| PVT16080 | Lifescience Market | 2 ug | EUR 325 |
 pGreen- Puro- pSIH1- H1- copGFP- T2A- Puro |
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| PVT11116 | Lifescience Market | 2 ug | EUR 370 |
 PB-Cuo-MCS-IRES-GFP-EF1-CymR-Puro Inducible cDNA Cloning and Expression Vector |
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| PBQM812A-1 | SBI | 10 ug | EUR 1096 |
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 MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid |
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| CASLV120PA-1 | SBI | 10 ug | EUR 697 |
|
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 pCDH-CuO-MCS-IRES-GFP-EF1-CymR-T2A-Puro All-in-one inducible IRES lentivector |
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| QM812B-1 | SBI | 10 ug | EUR 1310 |
|
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 plasmid) pCDH-CMV-MCS-EF1-Puro (CD510B) plasmid |
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| PVT17272 | Lifescience Market | 2 ug | EUR 474 |
 pCDF1-MCS1 cDNA Cloning and Expression Vector |
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| CD100A-1 | SBI | 10 ug | EUR 544 |
|
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 pCDH-CMV-GLUT1-EF1-turboRFP-T2A-Puro |
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| PVT13588 | Lifescience Market | 2 ug | EUR 599 |
 pCDH-ME-APOE-Luc2-T2A-tdTomato Plasmid |
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| PVT17253 | Lifescience Market | 2 ug | EUR 370 |
 pCDH-CuO-MCS-EF1-GFP+Puro dual promoter and dual marker lentivector |
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| QM513B-1 | SBI | 10 ug | EUR 679 |
|
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 pCDH-CuO-MCS-EF1-RFP+Puro dual promoter and dual marker lentivector |
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| QM516B-2 | SBI | 10 ug | EUR 679 |
|
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 pCDH-CuO-MCS-EF1-GFP dual promoter lentivector |
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| QM511B-1 | SBI | 10 ug | EUR 679 |
|
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 pCDH-CuO-MCS-EF1-RFP dual promoter lentivector |
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| QM512B-1 | SBI | 10 ug | EUR 679 |
|
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 pCDH-CMV-MCS-EF1-Puro-FLAG-HIF2 alpha |
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| PVT12166 | Lifescience Market | 2 ug | EUR 370 |
 EEV cloning and expression vector) Constitutive (CAGs promoter) EEV cloning and expression vector |
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| EEV600A-1 | SBI | 10 ug | EUR 677 |
|
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 EEV cloning and expression vector) Cumate-Inducible (CuO) EEV cloning and expression vector |
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| EEV610A-1 | SBI | 10 ug | EUR 677 |
|
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 pSIH1-H1-Puro shRNA Cloning and Expression Vector |
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| SI500A-1 | SBI | 10 ug | EUR 567 |
|
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 pSIH1-H1-H2Kk shRNA Cloning and Expression Vector |
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| SI502A-1 | SBI | 10 ug | EUR 567 |
|
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) pCDH-Cuo-RFP-T2A-GFP (positive control plasmid) |
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| QM350PA-1 | SBI | 10 ug | EUR 597 |
|
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) pCDH-Cuo-RFP-T2A-GFP (positive control virus) |
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| QM350VA-1 | SBI | >1 x 10^6 IFUs | EUR 636 |
|
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 pCDH-CuO-MCS-IRES-GFP co-inducible IRES lentivector |
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| QM530A-2 | SBI | 10 ug | EUR 679 |
|
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 pCDH-CuO-MCS-IRES-RFP co-inducible IRES lentivector |
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| QM531A-2 | SBI | 10 ug | EUR 679 |
|
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 XPack MSCV-XP-GFP-EF1-Puro Expression Lentivector |
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| XPAK730PA-1 | SBI | 10 ug | EUR 826 |
|
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 XPack MSCV-XP-GFP-EF1-Puro Expression Lentivirus |
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| XPAK730VA-1 | SBI | >1 x 10^6 IFUs | EUR 826 |
|
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 XPack MSCV-XP-Luciferase-EF1-Puro Expression Lentivector |
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| XPAK732PA-1 | SBI | 10 ug | EUR 826 |
|
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 XPack MSCV-XP-Luciferase-EF1-Puro Expression Lentivirus |
|||
| XPAK732VA-1 | SBI | >1 x 10^6 IFUs | EUR 826 |
|
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) pCDH-EF1a-Piranha-T2A-Puro virus (>1x10^6 IFUs) |
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| PTPD527VA-1 | SBI | >1x10^6 IFUs | EUR 697 |
 MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV120PA-KIT | SBI | 1 Kit | EUR 1132 |
|
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-T2A-Puro SmartNickase Lentivector Plasmid) Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid |
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| CASLV220PA-1 | SBI | 10 ug | EUR 697 |
|
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) Minicircle Dual Reporter: MSCV-RFP-T2A-Luciferase DNA (30 ug) |
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| BLIV506MC-1 | SBI | 30 ug | EUR 716 |
|
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) Minicircle Dual Reporter: MSCV-GFP-T2A-Luciferase DNA (30 ug) |
|||
| BLIV507MC-1 | SBI | 30 ug | EUR 716 |
|
|||
 pCDH-CMV-MCS-EF1-aRFP cDNA Clon/Exp Vector [pre-packaged] |
|||
| CD512VA-1 | SBI | >1 x 10^6 IFUs | EUR 786 |
|
|||
 pFC-EF1-MCS-pA-PGK-RFP-T2A-Puro PhiC31 Donor Vector |
|||
| FC550A-1 | SBI | 10ug | EUR 773 |
|
|||
 pFC-PGK-MCS-pA-EF1-GFP-T2A-Puro PhiC31 Donor Vector |
|||
| FC551A-1 | SBI | 10ug | EUR 773 |
|
|||
 pAAVK-EF1?-MCS1-CMV-MCS2 AAVanced Cloning and Expression Vector |
|||
| AAV503A-1 | SBI | 10 ug | EUR 677 |
|
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 pAAVK-EF1?-MCS1-CMV-EGFP AAVanced Cloning and Expression Vector |
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| AAV536A-1 | SBI | 10 ug | EUR 677 |
|
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 pAAVK-EF1?-MCS1-CMV-Puro AAVanced Cloning and Expression Vector |
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| AAV537A-1 | SBI | 10 ug | EUR 677 |
|
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 pAAVK-EF1?-MCS1-CMV-mRFP AAVanced Cloning and Expression Vector |
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| AAV538A-1 | SBI | 10 ug | EUR 677 |
|
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) Minicircle Dual Reporter: MSCV-RFP-T2A-Luciferase Parental Plasmid (10 ug) |
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| BLIV506MN-1 | SBI | 10 ug | EUR 972 |
|
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) Minicircle Dual Reporter: MSCV-GFP-T2A-Luciferase Parental Plasmid (10 ug) |
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| BLIV507MN-1 | SBI | 10 ug | EUR 972 |
|
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 pCDH-CMV-MCS-EF1-RFP+Puro cDNA Clon/Exp Vector [pre-packaged] |
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| CD516VB-2 | SBI | >1 x 10^6 IFUs | EUR 772 |
|
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 pCDF1-MCS2-EF1-Puro cDNA Cloning and Expression Lentivector |
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| CD110B-1 | SBI | 10 ug | EUR 635 |
|
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 MSCV-hspCas9-T2A-Puro SmartNuclease Pre-Packaged Lentiviral Particles [>10^6 IFUs] |
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| CASLV120VA-1 | SBI | 2 x 25ul | EUR 697 |
|
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) pCDH-CMV-Piranha-EF1-GFP-T2A-Puro virus (>1x10^6 IFUs) |
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| PTPD513VA-1 | SBI | >1x10^6 IFUs | EUR 697 |
 pCDH-CMV-MCS-EF1-Puro cDNA Vector cDNA Clon/Exp Vector [pre-packaged virus] |
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| CD510VB-1 | SBI | >1 x 10^6 IFUs | EUR 786 |
|
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 PCDH |
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| EF007042 | Lifescience Market | 96 Tests | EUR 689 |
PCDH |
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| EF007043 | Lifescience Market | 96 Tests | EUR 689 |
) pMC.CMV-GFP-H1-MCS (shRNA expression parental minicircle plasmid) |
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| MNSI500A-1 | SBI | 10 ug | EUR 847 |
|
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-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit) Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV220PA-KIT | SBI | 1 Kit | EUR 1132 |
|
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 MSCV-hspCas9-T2A-Puro-H1-gRNA linearized all-in-one SmartNuclease Lentivector Plasmid |
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| CASLV320PA-1 | SBI | 10 rxn | EUR 805 |
|
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) MSCV-Mouse Foxp3-EF1-GFP-T2A-Puro Stable Jurkat cell line (Puro resistant) |
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| TCL110C-1 | SBI | 5 x 10^6 cells/ml | EUR 1682 |
|
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) MSCV-Human Foxp3-EF1-GFP-T2A-Puro Stable Jurkat cell line (Puro resistant) |
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| TCL210C-1 | SBI | 5 x 10^6 cells/ml | EUR 1682 |
|
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) MSCV-Mouse RORgt-EF1-GFP-T2A-Puro Stable Jurkat cell line (Puro resistant) |
|||
| TCL310C-1 | SBI | 5 x 10^6 cells/ml | EUR 1682 |
|
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) MSCV-Human RORgt-EF1-GFP-T2A-Puro Stable Jurkat cell line (Puro resistant) |
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| TCL410C-1 | SBI | 5 x 10^6 cells/ml | EUR 1682 |
|
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 MSCV JMJD3 Plasmid |
|||
| PVT17098 | Lifescience Market | 2 ug | EUR 325 |
 XPack CMV-XP-MCS-EF1-Puro Cloning Lentivector |
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| XPAK510PA-1 | SBI | 10 ug | EUR 797 |
|
|||
 GFP-Fusion HR Targeting Vector [GFP-pA-LoxP-EF1?-RFP-T2A-Puro-pA-LoxP-MCS] |
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| HR120PA-1 | SBI | 10 ug | EUR 1023 |
|
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 GFP-Fusion HR Targeting Vector [GFP-pA-LoxP-EF1a-RFP-T2A-Hygro-pA-LoxP-MCS] |
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| HR220PA-1 | SBI | 10 ug | EUR 1023 |
|
|||
-T2A-Puro SmartNickase Pre-Packaged Lentiviral Particles [>10^6 IFUs]) Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Pre-Packaged Lentiviral Particles [>10^6 IFUs] |
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| CASLV220VA-1 | SBI | 2 x 25ul | EUR 697 |
|
|||
 PB-Cuo-shMCS-IRES-GFP-EF1-CymR-Puro Inducible shRNA Cloning and Expression Vector |
|||
| PBQMSH812A-1 | SBI | 10 ug | EUR 1096 |
|
|||
 pGEN- MCS |
|||
| PVT10565 | Lifescience Market | 2 ug | EUR 370 |
 pLEX- MCS |
|||
| PVT11092 | Lifescience Market | 2 ug | EUR 301 |
 MSCV-hspCas9-H1-gRNA SmartNuclease vector |
|||
| CAS960A-1 | SBI | 10 ug | EUR 805 |
|
|||
 PinPoint-HR attP Placement Vector ? Empty MCS for cloning HR Arms |
|||
| PIN400A-1 | SBI | 10ug | EUR 1268 |
|
|||
-T2A-Puro-H1-gRNA linearized all-in-one SmartNuclease Lentivector Plasmid) Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro-H1-gRNA linearized all-in-one SmartNuclease Lentivector Plasmid |
|||
| CASLV420PA-1 | SBI | 10 rxn | EUR 805 |
|
|||
 pCDH- CMV- IDH1 |
|||
| PVT10169 | Lifescience Market | 2 ug | EUR 301 |
 pCDH- CMV- CAMK2G |
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| PVT10240 | Lifescience Market | 2 ug | EUR 301 |
 pCDH- CMV- Rnd2 |
|||
| PVT10402 | Lifescience Market | 2 ug | EUR 301 |
 (pORF)) MCS ORF Vector (Human) (pORF) |
|||
| ORF023399 | ABM | 1.0 ug DNA | Ask for price |
 pMC.CMV-MCS-SV40polyA |
|||
| MN501A-1 | SBI | 10 ug | EUR 735 |
|
|||
 pMC.EF1-MCS-SV40polyA |
|||
| MN502A-1 | SBI | 10 ug | EUR 735 |
|
|||
 pMyosin-MCS-PSmOrange |
|||
| PVT17668 | Lifescience Market | 2 ug | EUR 383 |
 pECMV-MCS-Myc |
|||
| PVT17857 | Lifescience Market | 2 ug | EUR 341 |
 pLEGFP-MCS-FLAG |
|||
| PVT18164 | Lifescience Market | 2 ug | EUR 341 |
 pAAV- MCS Plasmid |
|||
| PVT2102 | Lifescience Market | 2 ug | EUR 241 |
 pT7- His- MCS |
|||
| PVT10595 | Lifescience Market | 2 ug | EUR 266 |
 pEU- E01- MCS |
|||
| PVT11379 | Lifescience Market | 2 ug | EUR 370 |
 pAAVS1-P-MCS |
|||
| PVT12296 | Lifescience Market | 2 ug | EUR 703 |
 pMD-AOX-MCS |
|||
| PVT14289 | Lifescience Market | 2 ug | EUR 911 |
 pCDNA3.1-MCS-6 |
|||
| PVT15973 | Lifescience Market | 2 ug | EUR 325 |
 pEnCMV-MCS-3 |
|||
| PVT17410 | Lifescience Market | 2 ug | EUR 300 |
) pPS-MSCV-GFP-RFP (plasmid) |
|||
| LV605PA-1 | SBI | 10 ug | EUR 656 |
|
|||
 PCDH- X/Y Antibody |
|||
| ABD8867 | Lifescience Market | 100 ug | EUR 438 |
 PCDH-X / Y Antibody |
|||
| 20-abx218014 | Abbexa |
|
|
|
|||
 PCDH-X/Y Antibody |
|||
| 45549-100ul | SAB | 100ul | EUR 252 |
PCDH-X/Y Antibody |
|||
| 45549-50ul | SAB | 50ul | EUR 187 |
PCDH-X/Y Antibody |
|||
| DF8867 | Affbiotech | 200ul | EUR 304 |
|
Description: PCDH-X/Y Antibody detects endogenous levels of total PCDH-X/Y. |
|||
 pCDH-513B-CTH Plasmid |
|||
| PVTB00700-4c | Lifescience Market | 2 ug | EUR 356 |
 pCDH-puro-CSF1R Plasmid |
|||
| PVTB00726-4a | Lifescience Market | 2 ug | EUR 356 |
 pCDH- puro- Bcl- XL |
|||
| PVT10290 | Lifescience Market | 2 ug | EUR 301 |
 pYLEX1 - Expression Vector |
|||
| FYY203-5MG | Yeastern Biotech | 5mg | Ask for price |
 pORB-MCS-sIRES-VSVG |
|||
| ABP-BM-sIRESVG | Allele Biotech | 5 ug | Ask for price |
|
|||
) MCS Recombinant Protein (Human) |
|||
| RP070194 | ABM | 100 ug | Ask for price |
 pCS2-EGFP-MCS-3 |
|||
| PVT17867 | Lifescience Market | 2 ug | EUR 300 |
CONCLUSION
Recombinant eukaryotic expression vector pCDH-FAP successfully constructed. These results provide a basis for further study on the function of FAP in vitro.
