Transplantation of neural stem cells (NSC) appears to be a promising regenerative therapies for various neurological disorders. However, NSC engraftment limited by the number of surviving cells. To maximize the effect of stem cell-mediated, time of implantation and the number of cells should be appropriately evaluated. Here, transgenic murine NSC line is optimized for high-level expression of the imaging reporter Luc2 and copGFP. NSC 150 000, 75 000, 15 000 or 1500 cell or Hanks buffered saline solution infused into the striatum of nude mice. NSCs survival was monitored by in vivo bioluminescence imaging (BLI) over 2 weeks and part of the brain that are histologically analyzed for the glial cells of the innate immune system.
Longitudinal Data vivo survival BLI revealed significantly reduced with the highest level of 150 000 engrafted NSCs. Cell loss is not correlated with the number of Iba-1 + immune cells or GFAP + astrocytes. histological quantification copGFP + cells at 14 days pasaimplantasi the data confirmed in vivo with the highest density copGFP + cells in 150-cell graft 000 and the highest survival rate for 1500 cells / graft. In conclusion, regenerative therapies should be rigorously evaluate the maximum number of stem cells to be transplanted in a single location, such as the results show that there is a critical limit cell is able to survive in the adult brain. Survival is limited by the availability of oxygen and nutrients, but not the inflammatory response caused by the implantation.
Gene therapy is a rapidly evolving field of molecular medicine. New, effective, and promoter-specific cancers in high demand by researchers seeking to treat cancer therapy via gene expression. Here, we are creating a combinatorial library of chimeric tumor-specific promoter module to identify new promoter with the desired function.
The library was constructed by combining the random fragments of the promoter of the eight human genes involved in the control of cell proliferation. Swimming promoter chimeric incorporated into lentiviral expression vector upstream CopGFP reporter genes, transduced into A431 cells, and enriched to an active promoter with cell sorting. Enriched library containing a very high proportion of active and tumor-specific promoter. This approach for generating combinatorial libraries of chimeric promoters may serve as a useful tool for selecting the promoter very specific and effective for cancer research and gene therapy.
Initial graft size and not the innate immune response limit survival of engrafted neural stem cells.
Transcriptome interstitial cells of Cajal express the unique gene signatures and selective.
Data scale transcriptome can reveal important clues in understanding the underlying molecular mechanism behind a particular cellular function and biological processes. Transcriptomics is a constantly evolving field of research use in biomarker discovery. Transcriptomic profile interstitial cells of Cajal (ICC), which serves as the slow-wave electrical pacemaker for gastrointestinal (GI) smooth muscle, has not been revealed.
ICC copGFP-label use of rats and flow cytometry, we isolated a population of ICC of murine small intestine and colon and obtain their transcriptome. In analyzing the transcriptome, we identified a set of unique markers including the ICC-restricted transcription factors, epigenetic enzyme / regulator, growth factors, receptors, protein kinase / phosphatase, and ion channels / transporters. This analysis provides new and unique insights into the cellular and biological functions of the ICC in GI physiology. In addition, we construct a genome browser interactive ICC
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.
PB-Cuo-shMCS-IRES-GFP-EF1-CymR-Puro Inducible shRNA Cloning and Expression Vector
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.
Description: The ANPRA/Aequorin expression vector is designed to co-express human atrial natriuretic peptide receptor A (ANPRA, also called natriuretic peptide receptor A/guanylate cyclase A) and jellyfish (Aequorea victoria) Aequorin in mammalian cells.
Description: GFP-Rac1 Expression Vector Set contains 3 vectors: Rac wild type, T17N dominant negative mutant, and Q61L constitutively active mutant. Each vector also contains a GFP reporter sequence.
Description: GFP-RhoA Expression Vector Set contains 3 vectors: RhoA wild type, T19N dominant negative mutant, and Q63L constitutively active mutant. Each vector also contains a GFP reporter sequence.
Description: GFP-Cdc42 Expression Vector Set contains 3 vectors: Cdc42 wild type, T17N dominant negative mutant, and Q61L constitutively active mutant. Each vector also contains a GFP reporter sequence.
Description: Active Rac1 Expression Vector Set contains 3 vectors expressing different constitutively active mutants of Rac1: Q61L, Q61L/F37A, and Q61L/Y40C.
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.
Description: Active H-Ras Expression Vector Set contains 3 vectors expressing different constitutively active mutants of H-Ras: V12, V12S35, and V12C40.
Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer.
Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer.
Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer.
SARS-CoV-2 Spike Glycoprotein Expression (S) Lentivector, pCDH-CMV-SARS-CoV-2-S-EF1α-Puro (Plasmid)
Description: Baculovirus cassette vector pAc-l-CH3 for the expression of human, humanized or chimeric IgG(lambda) in insect cells and secretion of assembled antibodies into the supernatant.
Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer.
Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer.
Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer.
SARS-CoV-2 Humanized Spike Glycoprotein (S) Expression Lentivector, pCDH-CMV-SARS-CoV-2-hS-EF1α-Puro (Plasmid)
Description: Making an adenovirus with traditional recombinant methods takes 2-3 months and requires tedious plaque recombination. More recent technologies have shortened this time somewhat, but still produce relatively high amounts of wild type (replication-competent) plaques, levels of which increase with serial amplification. The RAPAd® Adenoviral Expression Systems produce recombinant adenovirus with substantially reduced wild-type virus, while considerably shortening the production time to 2-3 weeks. Serial amplification of adenovirus produced using the RAPAd® system does not significantly increase replication-competent adenovirus levels. The system uses a backbone vector from which the 5' ITR, packaging signal and E1 sequences have been removed.
Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer.
Description: Clone your gene of interest and a gene-specific promoter into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293T or 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer.
Description: Making an adenovirus with traditional recombinant methods takes 2-3 months and requires tedious plaque recombination. More recent technologies have shortened this time somewhat, but still produce relatively high amounts of wild type (replication-competent) plaques, levels of which increase with serial amplification. The RAPAd® Adenoviral Expression Systems produce recombinant adenovirus with substantially reduced wild-type virus, while considerably shortening the production time to 2-3 weeks. Serial amplification of adenovirus produced using the RAPAd® system does not significantly increase replication-competent adenovirus levels. The system uses a backbone vector from which the 5' ITR, packaging signal and E1 sequences have been removed.
Super piggyBac Transposase expression vector (replaces PB200A-1), 50 rxns
Description: Pre-made over-expression lentivirus for human target: IL3RA, containing a RFP-Blasticidin dual selection marker.
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(http://med.unr.edu/physio/transcriptome) based UCSC genome database. To our knowledge, this is an online resource that provides a complete library of all known genetic transcripts expressed in primary ICC. Our genome browser offers a new perspective of an alternative expression of genes in the ICC and provide valuable reference for future functional studies.
Heather
