The Human IL-23 Decoy Receptor Inhibits T-Cells Producing IL-17 by Genetically Engineered Mesenchymal Stem Cells.

MRI Tracking of SPIO- and Fth1-Labeled Bone Marrow Mesenchymal Stromal Cell Transplantation for Treatment of Stroke.

The immunomodulatory and self-renewable features of human adipose mesenchymal stem cells (HAD-MSCs) mark their importance in regenerative medicine. Interleukin 23 (IL-23) as proinflammatory cytokines Hitting the regulatory T cells (Treg) and promote the response of T helper 17 (Th17) and helper 1 (Th1) cells T. This path begins inflammation and immunosuppression in several autoimmune diseases. The present study was to produce recombinant IL-23 decoy receptor (RIL- 23R) using own-MSCs as a good candidate for the former purpose vivo cell-based gene therapy reduces inflammation in autoimmune disease. have-MSCs isolated from lipoaspirate and then marked by differentiation.

RIL- 23R is designed and cloned into pCDH-813A- 1 lentiviral vectors. Transduction-MSCs have done in MOI (multiplicity of infection) = 50 with EFI pCDH- α- RIL- 23R- copGFP PGK. RIL- 23R expression and octamer-binding transcription factor 4 (OCT- 4) was determined by real-time polymerase chain reaction (real time-PCR). nature renews itself tested by OCT- 4. 23R designed RIL- bioactivity evaluated by IL-17 and IL-10 expression of splenocytes of mice. cell differentiation has confirmed the isolation right-MSCs from lipoaspirate.

Restriction enzyme digestion and sequencing verified the successful cloning RIL- 23R in CD813A-1 lentiviral vectors. Green fluorescent protein (GFP) positive transduction level is up to 90%, and real-time PCR showed expression levels of RIL-23R. October-4 has the same expression pattern nontransduced-MSCs have and have-MSCs transduced / RIL-23R which showed that lentiviral vectors does not affect the characteristics of own-MSCs. Downregulation of IL-17, and upregulation of IL-10 showed activity right-MSCs have engineered.

The results show that transduced own-MSCs / RIL- 23R, express IL-23 decoy receptors, may provide a useful approach to basic research on cell-based gene therapy for autoimmune disorders.

 The Human IL-23 Decoy Receptor Inhibits T-Cells Producing IL-17 by Genetically Engineered Mesenchymal Stem Cells.
The Human IL-23 Decoy Receptor Inhibits T-Cells Producing IL-17 by Genetically Engineered Mesenchymal Stem Cells.

Bioluminescent imaging exogenous Stem Cells in Intact Guinea Pig Cochlear.

Stem cell-based therapy can be used to replace damaged or missing neurons in the cochlear nerve of a patient suffering from sensorineural hearing loss severe-to-profound. In order to achieve functional recovery in future clinical trials, knowledge about the survival of transplanted cells and their differentiation into functional neurons is a prerequisite. It calls for non-invasive in vivo visualization of cell and long-term monitoring of life and their fate after transplantation cochlea. We have investigated if the molecular optical imaging allows visualization of cells within the cochlea exogenous intact cadaver heads guinea pigs.

Transduced (stem) cells stably co-expressing fluorescent (copGFP) and bioluminescent (Luc2) reporter molecules, which are injected into the internal auditory meatus or directly to the cochlea through the round window. After the injection of cells into the internal auditory meatus, glowing light signal observed in the cavum conchae of the ear, indicating that the light generated by Luc2 passing through the tympanic membrane and external auditory meatus.

pCDH-CMV-MCS-EF1-RFP cDNA Cloning and Expression Vector

CD512B-1 10 ug
EUR 762

pCDH-CMV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector

CD513B-1 10 ug
EUR 762

pCDH-CMV-MCS-EF1-Neo cDNA Cloning and Expression Vector

CD514B-1 10 ug
EUR 762

pCDH-CMV-MCS-EF1-Hygro cDNA Cloning and Expression Vector

CD515B-1 10 ug
EUR 762

pCDH-CMV-MCS-EF1-RFP+Puro cDNA Cloning and Expression Vector

CD516B-2 10 ug
EUR 762

pCDH-CMV-MCS cDNA Cloning and Expression Vector

CD500B-1 10 ug
EUR 652.8

pCDH-EF1-MCS-IRES-GFP cDNA Cloning and Expression Vector

CD530A-2 10 ug
EUR 783.6

pCDH-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector

CD531A-2 10 ug
EUR 783.6

pCDH-EF1-MCS-IRES-Puro cDNA Cloning and Expression Vector

CD532A-2 10 ug
EUR 783.6

pCDH-EF1-MCS-IRES-Neo cDNA Cloning and Expression Vector

CD533A-2 10 ug
EUR 783.6

pCDH-UbC-MCS-EF1-Hygro cDNA Cloning and Expression Vector

CD615B-1 10 ug
EUR 762

pCDH-MSCV-MCS-EF1-Puro cDNA Cloning and Expression Vector

CD710B-1 10 ug
EUR 762

pCDH-MSCV-MCS-EF1-GFP cDNA Cloning and Expression Vector

CD711B-1 10 ug
EUR 762

pCDH-MSCV-MCS-EF1-GFP+Puro cDNA Cloning and Expression Vector

CD713B-1 10 ug
EUR 762

pCDH- CMV- MCS- EF1- copGFP Plasmid

PVT2334 2 ug
EUR 390

pCDH-MCS-T2A-copGFP-MSCV Cloning and Expression Vector

CD523A-1 10 ug
EUR 783.6

pCDH- EF1- MCS- T2A- copGFP

PVT2336 2 ug
EUR 390

pCDH-EF1-MCS-T2A-Puro cDNA Cloning and Expression Vector 10 ug

CD527A-1 10 ug
EUR 783.6

pCDF1-MCS2-EF1-copGFP cDNA Cloning and Expression Vector

CD111B-1 10 ug
EUR 762

pCDH-EF1-MCS-(PGK-Puro) Cloning and Expression Vector

CD810A-1 10 ug
EUR 770.4

pCDH-EF1-MCS-(PGK-GFP) Cloning and Expression Vector

CD811A-1 10 ug
EUR 770.4

pCDH- CMV- MCS- EF1- CopGFP- T2A- Puro

PVT2333 2 ug
EUR 390

PB-CMV-MCS-EF1-Puro cDNA cloning and expression vector

PB510B-1 10 ug
EUR 842.4

PB-CMV-MCS-EF1-GFP cDNA cloning and expression vector

PB511B-1 10 ug
EUR 842.4

PB-CMV-MCS-EF1-RFP cDNA cloning and expression vector

PB512B-1 10 ug
EUR 842.4

PB-CMV-MCS-EF1-GreenPuro cDNA cloning and expression vector

PB513B-1 10 ug
EUR 842.4

PB-CMV-MCS-EF1-RedPuro cDNA Cloning and Expression Vector

PB514B-2 10 ug
EUR 842.4

pCDH-EF1-MCS-BGH-PGK-GFP-T2A-Puro cDNA Cloning and Expression Vector

CD550A-1 10 ug
EUR 750

pCDH-CMV-MCS-EF1-copGFP cDNA Clon/Exp Vector [pre-packaged virus]

CD511VB-1 >1 x 10^6 IFUs
EUR 943.2

pCDH-CMV-MCS-EF1-copGFP+Puro cDNA Clon/Exp Vector [pre-packaged]

CD513VB-1 >1 x 10^6 IFUs
EUR 943.2

pCDH-MCS-T2A-Puro-MSCV cDNA Cloning and Expression Vector

CD522A-1 10 ug
EUR 783.6

pCDH-UbC-MCS-IRES-GFP cDNA Cloning and Expression Vector

CD630A-1 10 ug
EUR 783.6

pCDH-MSCV-MCS-IRES-GFP cDNA Cloning and Expression Vector

CD731B-1 10 ug
EUR 783.6

pCDH-CMV-MCS-EF1-copGFP-T2A-Puro plasmid

PVT17271 2 ug
EUR 568.8

PB-EF1-MCS-IRES-GFP cDNA cloning and expression vector

PB530A-2 10 ug
EUR 842.4

PB-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector

PB531A-2 10 ug
EUR 842.4

PB-EF1-MCS-IRES-Neo cDNA cloning and expression vector

PB533A-2 10 ug
EUR 842.4

PB-MSCV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector

PB713B-1 10 ug
EUR 842.4

pCDH-CMV-MCS2 cDNA Cloning and Expression Vector

CD501A-1 10 ug
EUR 652.8

pCDH- MSCV- MCS- EF1- CopGFP- T2A- Puro

PVT11079 2 ug
EUR 444

pCDH-EF1-MCS-(PGK-GFP-T2A-Puro) Cloning and Expression Vector

CD813A-1 10 ug
EUR 770.4

pCDH-EF1-MCS-T2A-RFP (PGK-Puro) Cloning and Expression Vector

CD822A-1 10 ug
EUR 770.4

pCDH-EF1-MCS-T2A-GFP (PGK-Puro) Cloning and Expression Vector

CD823A-1 10 ug
EUR 770.4

pAAVK-EF1? -MCS AAVanced Cloning and Expression Vector

AAV502A-1 10 ug
EUR 812.4

pCDH- CMV- MCS- EF1- puro

PVT11075 2 ug
EUR 444

pCDH- CMV- MCS- EF1- Hygro

PVT11076 2 ug
EUR 444

pCDH-CMV-MCS-EF1-copGFP cDNA Clon/Exp Vector [pre-packaged Ultra-High Titer virus]

CD511VB-UHT >1x10^9 IFUs
EUR 1249.2

pCDH- CMV- mCherry- EF1- CopGFP- Puro

PVT11078 2 ug
EUR 444

pCDH-CMV-GSTA1-EF1-copGFP Plasmid

PVT16093 2 ug
EUR 390

Scramble control hairpin in pCDH-CMV-MCS-EF1-copGFP (CD511B-1)

PMIRH000PA-1 Bacterial Streak
EUR 787.2

pCDH-CMV-MCS-EF1-aRFP cDNA Clon/Exp Vector [pre-packaged]

CD512VA-1 >1 x 10^6 IFUs
EUR 943.2

pCDH-EF1-Luc-T2A-copGFP

PVT17649 2 ug
EUR 508.8

pCDH-CMV-MCS-EF1-EGFP-IRFP

PVT17586 2 ug
EUR 508.8

pCDH-CMV-MCS-EF1-Puro cDNA Vector cDNA Clon/Exp Vector [pre-packaged virus]

CD510VB-1 >1 x 10^6 IFUs
EUR 943.2

pCDH-CMV-Akaluc-EF1-CopGFP-T2A-Puro

PVT14490 2 ug
EUR 718.8

pCDH-CMV-exosome booster-EF1-copGFP-Puro vector

PVT12109 2 ug
EUR 1149.6

PB-Cuo-MCS-IRES-GFP-EF1-CymR-Puro Inducible cDNA Cloning and Expression Vector

PBQM812A-1 10 ug
EUR 1315.2

pAAVK-EF1? -MCS-T2A-EGFP AAVanced Cloning and Expression Vector

AAV526A-1 10 ug
EUR 812.4

pAAVK-EF1? -MCS-T2A-Puro AAVanced Cloning and Expression Vector

AAV527A-1 10 ug
EUR 812.4

pAAVK-EF1? ?MCS-T2A-mRFP AAVanced Cloning and Expression Vector

AAV528A-1 10 ug
EUR 812.4

PB-EF1-GreenPuro-H1-MCS shRNA cloning and expression vector

PBSI506A-1 10 ug
EUR 842.4

pCDH-CMV-MCS-EF1-RFP+Puro cDNA Clon/Exp Vector [pre-packaged]

CD516VB-2 >1 x 10^6 IFUs
EUR 926.4

pCDH-EF1-MCS*-T2A-GFP

CD526A-1 10 ug
EUR 783.6

pCDF1-MCS2-EF1-Puro cDNA Cloning and Expression Lentivector

CD110B-1 10 ug
EUR 762

pCDH- CMV- MCS- EF1- turboRFP- T2A- Puro

PVT11077 2 ug
EUR 444

pCDH-CMV-MCS-EF1-Puro (CD510B) plasmid

PVT17272 2 ug
EUR 568.8

pCDH-CMV-efemp1-m-EF1-CopGFP-T2A-Puro

PVT17438 2 ug
EUR 360

pCDH-CMV-TXNL1-HA-EF1-CopGFP-T2A-Puro

PVT17693 2 ug
EUR 309.6

pCDH-CMV-CCDC68-HA-EF1-CopGFP-T2A-Puro

PVT17760 2 ug
EUR 309.6

pCDH-CMV-ccl3-m-EF1-CopGFP-T2A-Puro

PVT19054 2 ug
EUR 360

pCDH-CMV-PTX3-EF1-CopGFP-T2A-Puro Plasmid

PVT17116 2 ug
EUR 390

pCDH- EF1- MCS- T2A- Puro Plasmid

PVT2335 2 ug
EUR 390

pCDH- ECMV- MCS- 3*Flag- EF1

PVT11080 2 ug
EUR 444

pCDH-CMV-MCS-EF1-Puro-FLAG-HIF2 alpha

PVT12166 2 ug
EUR 444

XStamp cloning and expression lentivector MSCV-Leader-MCS-C1C2-EF1-Puro

XSTP710PA-1 10 ug
EUR 956.4

pSIH1-H1-copGFP shRNA Cloning and Expression Vector

SI501A-1 10 ug
EUR 680.4

pCDF1-MCS1 cDNA Cloning and Expression Vector

CD100A-1 10 ug
EUR 652.8

pCDH-Cuo-MCS-EF1-CymR-T2A-Puro

QM800A-1 10 ug
EUR 1572

pCDH-EF1-MCS-T2A-Puro (CD520A) plasmid

PVT17273 2 ug
EUR 568.8

PB-CMV-GreenPuro-H1-MCS shRNA cloning and expression vector

PBSI505A-1 10 ug
EUR 842.4

pAAVK-EF1?-MCS1-CMV-MCS2 AAVanced Cloning and Expression Vector

AAV503A-1 10 ug
EUR 812.4

pAAVK-EF1?-MCS1-CMV-EGFP AAVanced Cloning and Expression Vector

AAV536A-1 10 ug
EUR 812.4

pAAVK-EF1?-MCS1-CMV-Puro AAVanced Cloning and Expression Vector

AAV537A-1 10 ug
EUR 812.4

pAAVK-EF1?-MCS1-CMV-mRFP AAVanced Cloning and Expression Vector

AAV538A-1 10 ug
EUR 812.4

pCDH-CuO-MCS-EF1-GFP dual promoter lentivector

QM511B-1 10 ug
EUR 814.8

pCDH-CuO-MCS-EF1-RFP dual promoter lentivector

QM512B-1 10 ug
EUR 814.8

pCDH-CuO-MCS empty cloning lentivector

QM500A-1 10 ug
EUR 716.4

XPack CMV-XP-MCS-EF1-Puro Cloning Lentivector

XPAK510PA-1 10 ug
EUR 956.4

pCDH-CuO-MCS-EF1-GFP+Puro dual promoter and dual marker lentivector

QM513B-1 10 ug
EUR 814.8

pCDH-CuO-MCS-EF1-RFP+Puro dual promoter and dual marker lentivector

QM516B-2 10 ug
EUR 814.8

pCDH-CMV-MCS-EF1a-GFP+BSD

PVT17282 2 ug
EUR 469.2

pCDH-CMV-exosome booster-EF1-Hygro

PVT12359 2 ug
EUR 3589.2

pCDH-CMV-Bach2-m-EF1-Puro

PVT13474 2 ug
EUR 843.6

pCDH-CMV-FGL1-EF1-Puro Plasmid

PVT16069 2 ug
EUR 390

pCDH-CMV-LAG3-EF1-Puro Plasmid

PVT16070 2 ug
EUR 390

pCDH-CMV-PDCD1-EF1-Hygro Plasmid

PVT16072 2 ug
EUR 390

pAAV-MCS Expression Vector

VPK-410 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pscAAV-MCS Expression Vector

VPK-430 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

XPack MSCV-XP-MCS-EF1-Puro Cloning Lentivector

XPAK710PA-1 10 ug
EUR 956.4

pCDH-CMV-3-FLAG-TOX3-EF1-Puro

PVT13401 2 ug
EUR 843.6

pCDH-CMV-GLUT1-EF1-turboRFP-T2A-Puro

PVT13588 2 ug
EUR 718.8

pCDH-CMV-mRFP1-EGFP-LC3B-EF1-Puro

PVT14019 2 ug
EUR 1093.2

pAAV-MCS Promoterless Expression Vector

VPK-411 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pCDH-EF1-Luc2-T2A-ZsGreen1

PVT17917 2 ug
EUR 508.8

pMC.CMV-MCS-EF1-GFP-SV40PolyA

MN511A-1 10 ug
EUR 1016.4

pMC.CMV-MCS-EF1-RFP-SV40PolyA

MN512A-1 10 ug
EUR 1016.4

PB-Cuo-shMCS-IRES-GFP-EF1-CymR-Puro Inducible shRNA Cloning and Expression Vector

PBQMSH812A-1 10 ug
EUR 1315.2

pCDH-EF1-CymR-T2A-Puro (plasmid)

QM200PA-2 10 ug
EUR 716.4

pCDH-EF1-CymR-T2A-Puro (virus)

QM200VA-2 >1 x 10^6 IFUs
EUR 763.2

pCDH-EF1-CymR-T2A-RFP (plasmid)

QM300PA-1 10 ug
EUR 716.4

pCDH-EF1-CymR-T2A-RFP (virus)

QM300VA-1 >1 x 10^6 IFUs
EUR 763.2

pCDH-EF1-CymR-T2A-Neo (plasmid)

QM400PA-2 10 ug
EUR 697.2

pCDH-EF1-CymR-T2A-Neo (virus)

QM400VA-2 >1 x 10^6 IFUs
EUR 744

pCDH- EF1- luc2- T2A- Tdtomato Plasmid

PVT2337 2 ug
EUR 289.2

pCDH-CuO-MCS-IRES-GFP-EF1-CymR-T2A-Puro All-in-one inducible IRES lentivector

QM812B-1 10 ug
EUR 1572

Constitutive (CAGs promoter) EEV cloning and expression vector

EEV600A-1 10 ug
EUR 812.4

Cumate-Inducible (CuO) EEV cloning and expression vector

EEV610A-1 10 ug
EUR 812.4

pSIH1-H1-Puro shRNA Cloning and Expression Vector

SI500A-1 10 ug
EUR 680.4

pSIH1-H1-H2Kk shRNA Cloning and Expression Vector

SI502A-1 10 ug
EUR 680.4

XPack CMV-XP-GFP-EF1-Puro Expression Lentivector

XPAK530PA-1 10 ug
EUR 991.2

XPack CMV-XP-GFP-EF1-Puro Expression Lentivirus

XPAK530VA-1 >1 x 10^6 IFUs
EUR 991.2

XPack CMV-XP-RFP-EF1-Puro Expression Lentivector

XPAK531PA-1 10 ug
EUR 991.2

XPack CMV-XP-Luciferase-EF1-Puro Expression Lentivector

XPAK532PA-1 10 ug
EUR 991.2

XPack CMV-XP-Luciferase-EF1-Puro Expression Lentivirus

XPAK532VA-1 >1 x 10^6 IFUs
EUR 991.2

pGreenPuro shRNA expression lentivector (EF1)

SI506A-1 10 ug
EUR 787.2

pRedPuro shRNA expression lentivector (EF1)

SI516A-1 10 ug
EUR 787.2

pCDH-CMV-Piranha-EF1-GFP-T2A-Puro virus (>1x10^6 IFUs)

PTPD513VA-1 >1x10^6 IFUs
EUR 836.4

pCDH- CMV- Rnd2

PVT10402 2 ug
EUR 361.2

pCDH- CMV- IDH1

PVT10169 2 ug
EUR 361.2

pCDH- CMV- CAMK2G

PVT10240 2 ug
EUR 361.2

pCDH-CuO-MCS-T2A-GFP co-inducible T2A lentivector

QM521A-1 10 ug
EUR 814.8

pCDH-CuO-MCS-T2A-RFP co-inducible T2A lentivector

QM522A-1 10 ug
EUR 814.8

pCDH-CuO-MCS-IRES-GFP co-inducible IRES lentivector

QM530A-2 10 ug
EUR 814.8

pCDH-CuO-MCS-IRES-RFP co-inducible IRES lentivector

QM531A-2 10 ug
EUR 814.8

T2A-GFP Co-Expression HR Targeting Vector [T2A-GFP-pA-loxP-EF1?-RFP-T2A-Puro-pA-LoxP-MCS]

HR130PA-1 10 ug
EUR 1227.6

pFC-EF1-MCS-pA-PGK-RFP-T2A-Puro PhiC31 Donor Vector

FC550A-1 10ug
EUR 927.6

pFC-PGK-MCS-pA-EF1-GFP-T2A-Puro PhiC31 Donor Vector

FC551A-1 10ug
EUR 927.6

GFP-T2A-Luciferase Co-Expression HR Targeting Vector [GFP-T2A-Luc-pA-loxP-EF1?-RFP-T2A-Puro-pA-LoxP-MCS]

HR150PA-1 10 ug
EUR 1227.6

XPack MSCV-XP-GFP-EF1-Puro Expression Lentivector

XPAK730PA-1 10 ug
EUR 991.2

XPack MSCV-XP-GFP-EF1-Puro Expression Lentivirus

XPAK730VA-1 >1 x 10^6 IFUs
EUR 991.2

XPack MSCV-XP-Luciferase-EF1-Puro Expression Lentivector

XPAK732PA-1 10 ug
EUR 991.2

XPack MSCV-XP-Luciferase-EF1-Puro Expression Lentivirus

XPAK732VA-1 >1 x 10^6 IFUs
EUR 991.2

PCDH

EF007042 96 Tests
EUR 826.8

PCDH

EF007043 96 Tests
EUR 826.8

pMC.CMV-GFP-H1-MCS (shRNA expression parental minicircle plasmid)

MNSI500A-1 10 ug
EUR 1016.4

pCDH-CMV-HA-ABCB1 Plasmid

PVTB00597-4a 2 ug
EUR 427.2

pHAGE-CMV-MCS-IRES-ZsGreen1

PVT14048 2 ug
EUR 843.6

pCT-CMV-GFP-MCS-EF1-Puro (for adding fusion proteins at the C-terminus of GFP)

CYTO800-PA-1 10 ug
EUR 820.8

pCT-CMV-MCS-GFP-EF1-Puro (for adding fusion proteins at the N-terminus of GFP)

CYTO801-PA-1 10 ug
EUR 820.8

pLenti-CMV-MCS-GFP-SV-puro

PVT13740 2 ug
EUR 718.8

pMK231 (AAVS1 CMV-MCS-Puro) Plasmid

PVT15992 2 ug
EUR 390

pMC.CMV-GFP-T2A-Puro-H1-MCS (shRNA expression parental minicircle plasmid)

MNSI505A-1 10 ug
EUR 1016.4

pMC.EF1-GFP-T2A-Puro-H1-MCS (shRNA expression parental minicircle plasmid)

MNSI506A-1 10 ug
EUR 1016.4

pGEN- MCS

PVT10565 2 ug
EUR 444

pLEX- MCS

PVT11092 2 ug
EUR 361.2

PinPoint-HR attP Placement Vector ? Empty MCS for cloning HR Arms

PIN400A-1 10ug
EUR 1521.6

GFP-Fusion HR Targeting Vector [GFP-pA-LoxP-EF1?-RFP-T2A-Puro-pA-LoxP-MCS]

HR120PA-1 10 ug
EUR 1227.6

AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS)

GE622A-1 10 ug
EUR 1443.6

CMV control lentivirus (Hygro)

CMV-Null-Hygro 1 x107 IFU/ml x 200ul
EUR 418.8
Description: Negative control lentivirus contains a null spacer insert under CMV promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It has the hygromycin selection under RSV promoter.

CMV control lentivirus (Zeo)

CMV-Null-Zeo 1 x107 IFU/ml x 200ul
EUR 418.8
Description: Negative control lentivirus contains a null spacer insert under CMV promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It has the Zeocin selection under RSV promoter.

pFC-CMV-MCS-pA-SV40-Neo PhiC31 Donor Vector

FC501A-1 10ug
EUR 927.6

pLenti-EF1a-EGFP-Puro-CMV-MCS-3Flag

PVT17274 2 ug
EUR 568.8

CMV Control lentiviral particles (Bsd)

CMV-Null-Bsd 1 x107 IFU/ml x 200ul
EUR 418.8
Description: Negative control lentivirus contains a null spacer insert under CMV promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It also has the blasticidin marker under RSV promoter.

CMV Control lentiviral particles (Neo)

CMV-Null-Neo 1 x107 IFU/ml x 200ul
EUR 418.8
Description: Negative control lentivirus contains a null spacer insert under CMV promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It also has the Neomycin marker under RSV promoter.

CMV Control lentiviral particles (Puro)

CMV-Null-Puro 1 x107 IFU/ml x 200ul
EUR 418.8
Description: Negative control lentivirus contains a null spacer insert under CMV promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It also has the Puromycin marker under RSV promoter.

BLIV 2.0 Reporter: CMV-Luciferase-EF1a-copGFP Lentivector Plasmid

BLIV511PA-1 10 ug
EUR 963.6

pMXs-EF1? Retroviral Vector

RTV-063 10 µg
EUR 748.8
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

MCS ORF Vector (Human) (pORF)

ORF023399 1.0 ug DNA Ask for price

One-Step gDNA Removal and cDNA Synthesis SuperMix

20-abx09885920ulSystems
  • EUR 1228.80
  • EUR 895.20
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

CMV Control lentiviral particles (GFP-Bsd)

CMV-Null-GB 1 x107 IFU/ml x 200ul
EUR 418.8
Description: Negative control lentivirus contains a null spacer insert under CMV promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It also has the GFP-Blasticidin fusion marker under RSV promoter.

CMV Control lentiviral particles (GFP-Puro)

CMV-Null-GP 1 x107 IFU/ml x 200ul
EUR 418.8
Description: Negative control lentivirus contains a null spacer insert under CMV promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It also has the GFP-Puromycin fusion marker under RSV promoter.

CMV Control lentiviral particles (RFP-Bsd)

CMV-Null-RB 1 x107 IFU/ml x 200ul
EUR 418.8
Description: Negative control lentivirus contains a null spacer insert under CMV promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It also has the RFP-Blasticidin fusion marker under RSV promoter.

CMV Control lentiviral particles (RFP-Puro)

CMV-Null-RP 1 x107 IFU/ml x 200ul
EUR 418.8
Description: Negative control lentivirus contains a null spacer insert under CMV promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It also has the RFP-Puromycin fusion marker under RSV promoter.

pLenti- EF1

PVT11083 2 ug
EUR 361.2

pMK1200- EF1

PVT11119 2 ug
EUR 361.2

PB-CMV-miR-302/367-EF1-GFP iPSC Vector

PB-MIR302 10 ug
EUR 2212.8

pMC.CMV-MCS-SV40polyA

MN501A-1 10 ug
EUR 882

pMC.EF1-MCS-SV40polyA

MN502A-1 10 ug
EUR 882

pEnCMV-MCS-3

PVT17410 2 ug
EUR 360

pMyosin-MCS-PSmOrange

PVT17668 2 ug
EUR 459.6

pECMV-MCS-Myc

PVT17857 2 ug
EUR 409.2

pLEGFP-MCS-FLAG

PVT18164 2 ug
EUR 409.2

pAAV- MCS Plasmid

PVT2102 2 ug
EUR 289.2

pT7- His- MCS

PVT10595 2 ug
EUR 319.2

pEU- E01- MCS

PVT11379 2 ug
EUR 444

pAAVS1-P-MCS

PVT12296 2 ug
EUR 843.6

pMD-AOX-MCS

PVT14289 2 ug
EUR 1093.2

pCDNA3.1-MCS-6

PVT15973 2 ug
EUR 390

pGreenPuro shRNA expression lentivector (CMV)

SI505A-1 10 ug
EUR 787.2

BLIV 2.0 Reporter: CMV-Luciferase-EF1a-copGFP Pre-packaged Virus

BLIV511VA-1 >2 x10^6 IFUs
EUR 963.6

Similar results were obtained after injection of the cells through the round window membrane, directly into the scala tympani canal or in Rosenthal in the modiolus of the cochlea basal turn. Imaging of the auditory bulla shows that bioluminescent signal passing through the tympanic membrane and the cracks in the wall of the bulla bone. After opening the auditory bulla, bioluminescent signal was coming from a round window. This is the first study showing that bioluminescence imaging allows visualization of luciferase-expressing cells injected into the intact guinea pig cochlea.

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