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The Human IL-23 Decoy Receptor Inhibits T-Cells Producing IL-17 by Genetically Engineered Mesenchymal Stem Cells.
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The immunomodulatory and self-renewable features of human adipose mesenchymal stem cells (HAD-MSCs) mark their importance in regenerative medicine. Interleukin 23 (IL-23) as proinflammatory cytokines Hitting the regulatory T cells (Treg) and promote the response of T helper 17 (Th17) and helper 1 (Th1) cells T. This path begins inflammation and immunosuppression in several autoimmune diseases. The present study was to produce recombinant IL-23 decoy receptor (RIL- 23R) using own-MSCs as a good candidate for the former purpose vivo cell-based gene therapy reduces inflammation in autoimmune disease. have-MSCs isolated from lipoaspirate and then marked by differentiation.
RIL- 23R is designed and cloned into pCDH-813A- 1 lentiviral vectors. Transduction-MSCs have done in MOI (multiplicity of infection) = 50 with EFI pCDH- α- RIL- 23R- copGFP PGK. RIL- 23R expression and octamer-binding transcription factor 4 (OCT- 4) was determined by real-time polymerase chain reaction (real time-PCR). nature renews itself tested by OCT- 4. 23R designed RIL- bioactivity evaluated by IL-17 and IL-10 expression of splenocytes of mice. cell differentiation has confirmed the isolation right-MSCs from lipoaspirate.
Restriction enzyme digestion and sequencing verified the successful cloning RIL- 23R in CD813A-1 lentiviral vectors. Green fluorescent protein (GFP) positive transduction level is up to 90%, and real-time PCR showed expression levels of RIL-23R. October-4 has the same expression pattern nontransduced-MSCs have and have-MSCs transduced / RIL-23R which showed that lentiviral vectors does not affect the characteristics of own-MSCs. Downregulation of IL-17, and upregulation of IL-10 showed activity right-MSCs have engineered.
The results show that transduced own-MSCs / RIL- 23R, express IL-23 decoy receptors, may provide a useful approach to basic research on cell-based gene therapy for autoimmune disorders.
Bioluminescent imaging exogenous Stem Cells in Intact Guinea Pig Cochlear.
Stem cell-based therapy can be used to replace damaged or missing neurons in the cochlear nerve of a patient suffering from sensorineural hearing loss severe-to-profound. In order to achieve functional recovery in future clinical trials, knowledge about the survival of transplanted cells and their differentiation into functional neurons is a prerequisite. It calls for non-invasive in vivo visualization of cell and long-term monitoring of life and their fate after transplantation cochlea. We have investigated if the molecular optical imaging allows visualization of cells within the cochlea exogenous intact cadaver heads guinea pigs.
Transduced (stem) cells stably co-expressing fluorescent (copGFP) and bioluminescent (Luc2) reporter molecules, which are injected into the internal auditory meatus or directly to the cochlea through the round window. After the injection of cells into the internal auditory meatus, glowing light signal observed in the cavum conchae of the ear, indicating that the light generated by Luc2 passing through the tympanic membrane and external auditory meatus.
 pCDH-CMV-MCS-t2A-copGFP cDNA Cloning and Expression Vector |
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| CD524A-1 | SBI | 10 ug | EUR 653 |
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 pCDH-CMV-MCS-EF1-Puro cDNA Cloning and Expression Vector |
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| CD510B-1 | SBI | 10 ug | EUR 635 |
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 pCDH-CMV-MCS-EF1-RFP cDNA Cloning and Expression Vector |
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| CD512B-1 | SBI | 10 ug | EUR 635 |
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 pCDH-CMV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector |
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| CD513B-1 | SBI | 10 ug | EUR 635 |
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 pCDH-CMV-MCS-EF1-Neo cDNA Cloning and Expression Vector |
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| CD514B-1 | SBI | 10 ug | EUR 635 |
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 pCDH-CMV-MCS-EF1-Hygro cDNA Cloning and Expression Vector |
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| CD515B-1 | SBI | 10 ug | EUR 635 |
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 pCDH-CMV-MCS-EF1-RFP+Puro cDNA Cloning and Expression Vector |
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| CD516B-2 | SBI | 10 ug | EUR 635 |
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 pCDH-EF1-MCS-IRES-GFP cDNA Cloning and Expression Vector |
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| CD530A-2 | SBI | 10 ug | EUR 653 |
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 pCDH-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector |
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| CD531A-2 | SBI | 10 ug | EUR 653 |
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 pCDH-EF1-MCS-IRES-Puro cDNA Cloning and Expression Vector |
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| CD532A-2 | SBI | 10 ug | EUR 653 |
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 pCDH-EF1-MCS-IRES-Neo cDNA Cloning and Expression Vector |
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| CD533A-2 | SBI | 10 ug | EUR 653 |
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 pCDH-UbC-MCS-EF1-Hygro cDNA Cloning and Expression Vector |
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| CD615B-1 | SBI | 10 ug | EUR 635 |
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 pCDH-MSCV-MCS-EF1-Puro cDNA Cloning and Expression Vector |
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| CD710B-1 | SBI | 10 ug | EUR 635 |
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 pCDH-MSCV-MCS-EF1-GFP cDNA Cloning and Expression Vector |
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| CD711B-1 | SBI | 10 ug | EUR 635 |
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 pCDH-CMV-MCS cDNA Cloning and Expression Vector |
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| CD500B-1 | SBI | 10 ug | EUR 544 |
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 pCDH- CMV- MCS- EF1- copGFP Plasmid |
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| PVT2334 | Lifescience Market | 2 ug | EUR 325 |
 pCDH-MSCV-MCS-EF1-GFP+Puro cDNA Cloning and Expression Vector |
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| CD713B-1 | SBI | 10 ug | EUR 635 |
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 pCDH- EF1- MCS- T2A- copGFP |
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| PVT2336 | Lifescience Market | 2 ug | EUR 325 |
 pCDH-MCS-T2A-copGFP-MSCV Cloning and Expression Vector |
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| CD523A-1 | SBI | 10 ug | EUR 653 |
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 pCDH-EF1-MCS-T2A-Puro cDNA Cloning and Expression Vector 10 ug |
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| CD527A-1 | SBI | 10 ug | EUR 653 |
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 pCDH- CMV- MCS- EF1- CopGFP- T2A- Puro |
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| PVT2333 | Lifescience Market | 2 ug | EUR 325 |
 pCDF1-MCS2-EF1-copGFP cDNA Cloning and Expression Vector |
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| CD111B-1 | SBI | 10 ug | EUR 635 |
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 Cloning and Expression Vector) pCDH-EF1-MCS-(PGK-Puro) Cloning and Expression Vector |
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| CD810A-1 | SBI | 10 ug | EUR 642 |
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 Cloning and Expression Vector) pCDH-EF1-MCS-(PGK-GFP) Cloning and Expression Vector |
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| CD811A-1 | SBI | 10 ug | EUR 642 |
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 PB-CMV-MCS-EF1-Puro cDNA cloning and expression vector |
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| PB510B-1 | SBI | 10 ug | EUR 702 |
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 PB-CMV-MCS-EF1-GFP cDNA cloning and expression vector |
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| PB511B-1 | SBI | 10 ug | EUR 702 |
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 PB-CMV-MCS-EF1-RFP cDNA cloning and expression vector |
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| PB512B-1 | SBI | 10 ug | EUR 702 |
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 PB-CMV-MCS-EF1-GreenPuro cDNA cloning and expression vector |
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| PB513B-1 | SBI | 10 ug | EUR 702 |
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 PB-CMV-MCS-EF1-RedPuro cDNA Cloning and Expression Vector |
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| PB514B-2 | SBI | 10 ug | EUR 702 |
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 pCDH-EF1-MCS-BGH-PGK-GFP-T2A-Puro cDNA Cloning and Expression Vector |
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| CD550A-1 | SBI | 10 ug | EUR 625 |
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 pCDH-CMV-MCS-EF1-copGFP cDNA Clon/Exp Vector [pre-packaged virus] |
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| CD511VB-1 | SBI | >1 x 10^6 IFUs | EUR 786 |
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 pCDH-CMV-MCS-EF1-copGFP+Puro cDNA Clon/Exp Vector [pre-packaged] |
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| CD513VB-1 | SBI | >1 x 10^6 IFUs | EUR 786 |
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 pCDH-CMV-MCS-EF1-copGFP-T2A-Puro plasmid |
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| PVT17271 | Lifescience Market | 2 ug | EUR 474 |
 pCDH-MCS-T2A-Puro-MSCV cDNA Cloning and Expression Vector |
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| CD522A-1 | SBI | 10 ug | EUR 653 |
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 pCDH-UbC-MCS-IRES-GFP cDNA Cloning and Expression Vector |
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| CD630A-1 | SBI | 10 ug | EUR 653 |
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 pCDH-MSCV-MCS-IRES-GFP cDNA Cloning and Expression Vector |
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| CD731B-1 | SBI | 10 ug | EUR 653 |
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 pCDH- MSCV- MCS- EF1- CopGFP- T2A- Puro |
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| PVT11079 | Lifescience Market | 2 ug | EUR 370 |
 PB-EF1-MCS-IRES-GFP cDNA cloning and expression vector |
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| PB530A-2 | SBI | 10 ug | EUR 702 |
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 PB-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector |
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| PB531A-2 | SBI | 10 ug | EUR 702 |
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 PB-EF1-MCS-IRES-Neo cDNA cloning and expression vector |
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| PB533A-2 | SBI | 10 ug | EUR 702 |
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 PB-MSCV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector |
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| PB713B-1 | SBI | 10 ug | EUR 702 |
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 pCDH-CMV-MCS2 cDNA Cloning and Expression Vector |
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| CD501A-1 | SBI | 10 ug | EUR 544 |
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 Cloning and Expression Vector) pCDH-EF1-MCS-(PGK-GFP-T2A-Puro) Cloning and Expression Vector |
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| CD813A-1 | SBI | 10 ug | EUR 642 |
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 Cloning and Expression Vector) pCDH-EF1-MCS-T2A-RFP (PGK-Puro) Cloning and Expression Vector |
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| CD822A-1 | SBI | 10 ug | EUR 642 |
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 Cloning and Expression Vector) pCDH-EF1-MCS-T2A-GFP (PGK-Puro) Cloning and Expression Vector |
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| CD823A-1 | SBI | 10 ug | EUR 642 |
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 pCDH- CMV- MCS- EF1- puro |
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| PVT11075 | Lifescience Market | 2 ug | EUR 370 |
 pCDH- CMV- MCS- EF1- Hygro |
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| PVT11076 | Lifescience Market | 2 ug | EUR 370 |
 pAAVK-EF1? -MCS AAVanced Cloning and Expression Vector |
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| AAV502A-1 | SBI | 10 ug | EUR 677 |
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 pCDH-CMV-MCS-EF1-copGFP cDNA Clon/Exp Vector [pre-packaged Ultra-High Titer virus] |
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| CD511VB-UHT | SBI | >1x10^9 IFUs | EUR 1041 |
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 pCDH- CMV- mCherry- EF1- CopGFP- Puro |
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| PVT11078 | Lifescience Market | 2 ug | EUR 370 |
 pCDH-CMV-GSTA1-EF1-copGFP Plasmid |
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| PVT16093 | Lifescience Market | 2 ug | EUR 325 |
) Scramble control hairpin in pCDH-CMV-MCS-EF1-copGFP (CD511B-1) |
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| PMIRH000PA-1 | SBI | Bacterial Streak | EUR 656 |
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 pCDH-EF1-Luc-T2A-copGFP |
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| PVT17649 | Lifescience Market | 2 ug | EUR 424 |
 pCDH-CMV-MCS-EF1-EGFP-IRFP |
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| PVT17586 | Lifescience Market | 2 ug | EUR 424 |
 pCDH-CMV-MCS-EF1-aRFP cDNA Clon/Exp Vector [pre-packaged] |
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| CD512VA-1 | SBI | >1 x 10^6 IFUs | EUR 786 |
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 pCDH-CMV-Akaluc-EF1-CopGFP-T2A-Puro |
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| PVT14490 | Lifescience Market | 2 ug | EUR 599 |
 pCDH-CMV-MCS-EF1-Puro cDNA Vector cDNA Clon/Exp Vector [pre-packaged virus] |
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| CD510VB-1 | SBI | >1 x 10^6 IFUs | EUR 786 |
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 pCDH-CMV-exosome booster-EF1-copGFP-Puro vector |
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| PVT12109 | Lifescience Market | 2 ug | EUR 958 |
 PB-Cuo-MCS-IRES-GFP-EF1-CymR-Puro Inducible cDNA Cloning and Expression Vector |
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| PBQM812A-1 | SBI | 10 ug | EUR 1096 |
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 pCDH-EF1-MCS*-T2A-GFP |
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| CD526A-1 | SBI | 10 ug | EUR 653 |
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 pAAVK-EF1? -MCS-T2A-EGFP AAVanced Cloning and Expression Vector |
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| AAV526A-1 | SBI | 10 ug | EUR 677 |
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 pAAVK-EF1? -MCS-T2A-Puro AAVanced Cloning and Expression Vector |
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| AAV527A-1 | SBI | 10 ug | EUR 677 |
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 pAAVK-EF1? ?MCS-T2A-mRFP AAVanced Cloning and Expression Vector |
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| AAV528A-1 | SBI | 10 ug | EUR 677 |
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 PB-EF1-GreenPuro-H1-MCS shRNA cloning and expression vector |
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| PBSI506A-1 | SBI | 10 ug | EUR 702 |
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 pCDH-CMV-MCS-EF1-RFP+Puro cDNA Clon/Exp Vector [pre-packaged] |
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| CD516VB-2 | SBI | >1 x 10^6 IFUs | EUR 772 |
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 pCDH- CMV- MCS- EF1- turboRFP- T2A- Puro |
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| PVT11077 | Lifescience Market | 2 ug | EUR 370 |
 plasmid) pCDH-CMV-MCS-EF1-Puro (CD510B) plasmid |
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| PVT17272 | Lifescience Market | 2 ug | EUR 474 |
 pCDH-CMV-TXNL1-HA-EF1-CopGFP-T2A-Puro |
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| PVT17693 | Lifescience Market | 2 ug | EUR 258 |
 pCDH-CMV-CCDC68-HA-EF1-CopGFP-T2A-Puro |
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| PVT17760 | Lifescience Market | 2 ug | EUR 258 |
 pCDH-CMV-ccl3-m-EF1-CopGFP-T2A-Puro |
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| PVT19054 | Lifescience Market | 2 ug | EUR 300 |
 pCDH-CMV-PTX3-EF1-CopGFP-T2A-Puro Plasmid |
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| PVT17116 | Lifescience Market | 2 ug | EUR 325 |
 pCDH-CMV-efemp1-m-EF1-CopGFP-T2A-Puro |
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| PVT17438 | Lifescience Market | 2 ug | EUR 300 |
 pCDF1-MCS2-EF1-Puro cDNA Cloning and Expression Lentivector |
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| CD110B-1 | SBI | 10 ug | EUR 635 |
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 pCDH- EF1- MCS- T2A- Puro Plasmid |
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| PVT2335 | Lifescience Market | 2 ug | EUR 325 |
 pCDH- ECMV- MCS- 3*Flag- EF1 |
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| PVT11080 | Lifescience Market | 2 ug | EUR 370 |
 pCDH-CMV-MCS-EF1-Puro-FLAG-HIF2 alpha |
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| PVT12166 | Lifescience Market | 2 ug | EUR 370 |
 XStamp cloning and expression lentivector MSCV-Leader-MCS-C1C2-EF1-Puro |
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| XSTP710PA-1 | SBI | 10 ug | EUR 797 |
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 pSIH1-H1-copGFP shRNA Cloning and Expression Vector |
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| SI501A-1 | SBI | 10 ug | EUR 567 |
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 pCDH-Cuo-MCS-EF1-CymR-T2A-Puro |
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| QM800A-1 | SBI | 10 ug | EUR 1310 |
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 plasmid) pCDH-EF1-MCS-T2A-Puro (CD520A) plasmid |
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| PVT17273 | Lifescience Market | 2 ug | EUR 474 |
 pCDF1-MCS1 cDNA Cloning and Expression Vector |
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| CD100A-1 | SBI | 10 ug | EUR 544 |
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 PB-CMV-GreenPuro-H1-MCS shRNA cloning and expression vector |
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| PBSI505A-1 | SBI | 10 ug | EUR 702 |
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 pCDH-CuO-MCS-EF1-GFP dual promoter lentivector |
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| QM511B-1 | SBI | 10 ug | EUR 679 |
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 pCDH-CuO-MCS-EF1-RFP dual promoter lentivector |
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| QM512B-1 | SBI | 10 ug | EUR 679 |
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 pAAVK-EF1?-MCS1-CMV-MCS2 AAVanced Cloning and Expression Vector |
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| AAV503A-1 | SBI | 10 ug | EUR 677 |
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 pAAVK-EF1?-MCS1-CMV-EGFP AAVanced Cloning and Expression Vector |
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| AAV536A-1 | SBI | 10 ug | EUR 677 |
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 pAAVK-EF1?-MCS1-CMV-Puro AAVanced Cloning and Expression Vector |
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| AAV537A-1 | SBI | 10 ug | EUR 677 |
|
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 pAAVK-EF1?-MCS1-CMV-mRFP AAVanced Cloning and Expression Vector |
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| AAV538A-1 | SBI | 10 ug | EUR 677 |
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 pCDH-CuO-MCS empty cloning lentivector |
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| QM500A-1 | SBI | 10 ug | EUR 597 |
|
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 pCDH-CuO-MCS-EF1-GFP+Puro dual promoter and dual marker lentivector |
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| QM513B-1 | SBI | 10 ug | EUR 679 |
|
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 pCDH-CuO-MCS-EF1-RFP+Puro dual promoter and dual marker lentivector |
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| QM516B-2 | SBI | 10 ug | EUR 679 |
|
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 XPack CMV-XP-MCS-EF1-Puro Cloning Lentivector |
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| XPAK510PA-1 | SBI | 10 ug | EUR 797 |
|
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 pCDH-CMV-MCS-EF1a-GFP+BSD |
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| PVT17282 | Lifescience Market | 2 ug | EUR 391 |
 pCDH-CMV-exosome booster-EF1-Hygro |
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| PVT12359 | Lifescience Market | 2 ug | EUR 2991 |
 pCDH-CMV-Bach2-m-EF1-Puro |
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| PVT13474 | Lifescience Market | 2 ug | EUR 703 |
 pCDH-CMV-FGL1-EF1-Puro Plasmid |
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| PVT16069 | Lifescience Market | 2 ug | EUR 325 |
 pCDH-CMV-LAG3-EF1-Puro Plasmid |
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| PVT16070 | Lifescience Market | 2 ug | EUR 325 |
 pCDH-CMV-PDCD1-EF1-Hygro Plasmid |
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| PVT16072 | Lifescience Market | 2 ug | EUR 325 |
 pAAV-MCS Expression Vector |
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| VPK-410 | Cell Biolabs | 10 µg | EUR 647 |
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Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV. |
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 pscAAV-MCS Expression Vector |
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| VPK-430 | Cell Biolabs | 10 µg | EUR 647 |
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Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV. |
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 XPack MSCV-XP-MCS-EF1-Puro Cloning Lentivector |
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| XPAK710PA-1 | SBI | 10 ug | EUR 797 |
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 pCDH-CMV-3-FLAG-TOX3-EF1-Puro |
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| PVT13401 | Lifescience Market | 2 ug | EUR 703 |
 pCDH-CMV-GLUT1-EF1-turboRFP-T2A-Puro |
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| PVT13588 | Lifescience Market | 2 ug | EUR 599 |
 pCDH-CMV-mRFP1-EGFP-LC3B-EF1-Puro |
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| PVT14019 | Lifescience Market | 2 ug | EUR 911 |
 pCDH-EF1-Luc2-T2A-ZsGreen1 |
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| PVT17917 | Lifescience Market | 2 ug | EUR 424 |
 pAAV-MCS Promoterless Expression Vector |
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| VPK-411 | Cell Biolabs | 10 µg | EUR 647 |
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Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV. |
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 pMC.CMV-MCS-EF1-GFP-SV40PolyA |
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| MN511A-1 | SBI | 10 ug | EUR 847 |
|
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 pMC.CMV-MCS-EF1-RFP-SV40PolyA |
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| MN512A-1 | SBI | 10 ug | EUR 847 |
|
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 pCDH- EF1- luc2- T2A- Tdtomato Plasmid |
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| PVT2337 | Lifescience Market | 2 ug | EUR 241 |
) pCDH-EF1-CymR-T2A-Puro (plasmid) |
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| QM200PA-2 | SBI | 10 ug | EUR 597 |
|
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) pCDH-EF1-CymR-T2A-Puro (virus) |
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| QM200VA-2 | SBI | >1 x 10^6 IFUs | EUR 636 |
|
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) pCDH-EF1-CymR-T2A-RFP (plasmid) |
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| QM300PA-1 | SBI | 10 ug | EUR 597 |
|
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) pCDH-EF1-CymR-T2A-RFP (virus) |
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| QM300VA-1 | SBI | >1 x 10^6 IFUs | EUR 636 |
|
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) pCDH-EF1-CymR-T2A-Neo (plasmid) |
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| QM400PA-2 | SBI | 10 ug | EUR 581 |
|
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) pCDH-EF1-CymR-T2A-Neo (virus) |
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| QM400VA-2 | SBI | >1 x 10^6 IFUs | EUR 620 |
|
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 PB-Cuo-shMCS-IRES-GFP-EF1-CymR-Puro Inducible shRNA Cloning and Expression Vector |
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| PBQMSH812A-1 | SBI | 10 ug | EUR 1096 |
|
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 pCDH-CuO-MCS-IRES-GFP-EF1-CymR-T2A-Puro All-in-one inducible IRES lentivector |
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| QM812B-1 | SBI | 10 ug | EUR 1310 |
|
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) pGreenPuro shRNA expression lentivector (EF1) |
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| SI506A-1 | SBI | 10 ug | EUR 656 |
|
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) pRedPuro shRNA expression lentivector (EF1) |
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| SI516A-1 | SBI | 10 ug | EUR 656 |
|
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 XPack CMV-XP-GFP-EF1-Puro Expression Lentivector |
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| XPAK530PA-1 | SBI | 10 ug | EUR 826 |
|
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 XPack CMV-XP-GFP-EF1-Puro Expression Lentivirus |
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| XPAK530VA-1 | SBI | >1 x 10^6 IFUs | EUR 826 |
|
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 XPack CMV-XP-RFP-EF1-Puro Expression Lentivector |
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| XPAK531PA-1 | SBI | 10 ug | EUR 826 |
|
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 XPack CMV-XP-Luciferase-EF1-Puro Expression Lentivector |
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| XPAK532PA-1 | SBI | 10 ug | EUR 826 |
|
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 XPack CMV-XP-Luciferase-EF1-Puro Expression Lentivirus |
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| XPAK532VA-1 | SBI | >1 x 10^6 IFUs | EUR 826 |
|
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 EEV cloning and expression vector) Constitutive (CAGs promoter) EEV cloning and expression vector |
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| EEV600A-1 | SBI | 10 ug | EUR 677 |
|
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 EEV cloning and expression vector) Cumate-Inducible (CuO) EEV cloning and expression vector |
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| EEV610A-1 | SBI | 10 ug | EUR 677 |
|
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 pSIH1-H1-Puro shRNA Cloning and Expression Vector |
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| SI500A-1 | SBI | 10 ug | EUR 567 |
|
|||
 pSIH1-H1-H2Kk shRNA Cloning and Expression Vector |
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| SI502A-1 | SBI | 10 ug | EUR 567 |
|
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) pCDH-CMV-Piranha-EF1-GFP-T2A-Puro virus (>1x10^6 IFUs) |
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| PTPD513VA-1 | SBI | >1x10^6 IFUs | EUR 697 |
 pCDH-CuO-MCS-T2A-GFP co-inducible T2A lentivector |
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| QM521A-1 | SBI | 10 ug | EUR 679 |
|
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 pCDH-CuO-MCS-T2A-RFP co-inducible T2A lentivector |
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| QM522A-1 | SBI | 10 ug | EUR 679 |
|
|||
 pCDH-CuO-MCS-IRES-GFP co-inducible IRES lentivector |
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| QM530A-2 | SBI | 10 ug | EUR 679 |
|
|||
 pCDH-CuO-MCS-IRES-RFP co-inducible IRES lentivector |
|||
| QM531A-2 | SBI | 10 ug | EUR 679 |
|
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 pCDH- CMV- IDH1 |
|||
| PVT10169 | Lifescience Market | 2 ug | EUR 301 |
 pCDH- CMV- CAMK2G |
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| PVT10240 | Lifescience Market | 2 ug | EUR 301 |
 pCDH- CMV- Rnd2 |
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| PVT10402 | Lifescience Market | 2 ug | EUR 301 |
 T2A-GFP Co-Expression HR Targeting Vector [T2A-GFP-pA-loxP-EF1?-RFP-T2A-Puro-pA-LoxP-MCS] |
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| HR130PA-1 | SBI | 10 ug | EUR 1023 |
|
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 pFC-EF1-MCS-pA-PGK-RFP-T2A-Puro PhiC31 Donor Vector |
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| FC550A-1 | SBI | 10ug | EUR 773 |
|
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 pFC-PGK-MCS-pA-EF1-GFP-T2A-Puro PhiC31 Donor Vector |
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| FC551A-1 | SBI | 10ug | EUR 773 |
|
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 GFP-T2A-Luciferase Co-Expression HR Targeting Vector [GFP-T2A-Luc-pA-loxP-EF1?-RFP-T2A-Puro-pA-LoxP-MCS] |
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| HR150PA-1 | SBI | 10 ug | EUR 1023 |
|
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 PCDH |
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| EF007042 | Lifescience Market | 96 Tests | EUR 689 |
PCDH |
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| EF007043 | Lifescience Market | 96 Tests | EUR 689 |
 XPack MSCV-XP-GFP-EF1-Puro Expression Lentivector |
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| XPAK730PA-1 | SBI | 10 ug | EUR 826 |
|
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 XPack MSCV-XP-GFP-EF1-Puro Expression Lentivirus |
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| XPAK730VA-1 | SBI | >1 x 10^6 IFUs | EUR 826 |
|
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 XPack MSCV-XP-Luciferase-EF1-Puro Expression Lentivector |
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| XPAK732PA-1 | SBI | 10 ug | EUR 826 |
|
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 XPack MSCV-XP-Luciferase-EF1-Puro Expression Lentivirus |
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| XPAK732VA-1 | SBI | >1 x 10^6 IFUs | EUR 826 |
|
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) pMC.CMV-GFP-H1-MCS (shRNA expression parental minicircle plasmid) |
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| MNSI500A-1 | SBI | 10 ug | EUR 847 |
|
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 pCDH-CMV-HA-ABCB1 Plasmid |
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| PVTB00597-4a | Lifescience Market | 2 ug | EUR 356 |
 pHAGE-CMV-MCS-IRES-ZsGreen1 |
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| PVT14048 | Lifescience Market | 2 ug | EUR 703 |
) pCT-CMV-GFP-MCS-EF1-Puro (for adding fusion proteins at the C-terminus of GFP) |
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| CYTO800-PA-1 | SBI | 10 ug | EUR 684 |
|
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) pCT-CMV-MCS-GFP-EF1-Puro (for adding fusion proteins at the N-terminus of GFP) |
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| CYTO801-PA-1 | SBI | 10 ug | EUR 684 |
|
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) pMC.CMV-GFP-T2A-Puro-H1-MCS (shRNA expression parental minicircle plasmid) |
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| MNSI505A-1 | SBI | 10 ug | EUR 847 |
|
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) pMC.EF1-GFP-T2A-Puro-H1-MCS (shRNA expression parental minicircle plasmid) |
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| MNSI506A-1 | SBI | 10 ug | EUR 847 |
|
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 GFP-Fusion HR Targeting Vector [GFP-pA-LoxP-EF1?-RFP-T2A-Puro-pA-LoxP-MCS] |
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| HR120PA-1 | SBI | 10 ug | EUR 1023 |
|
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) AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS) |
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| GE622A-1 | SBI | 10 ug | EUR 1203 |
|
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 pGEN- MCS |
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| PVT10565 | Lifescience Market | 2 ug | EUR 370 |
 pLEX- MCS |
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| PVT11092 | Lifescience Market | 2 ug | EUR 301 |
 pLenti-CMV-MCS-GFP-SV-puro |
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| PVT13740 | Lifescience Market | 2 ug | EUR 599 |
 Plasmid) pMK231 (AAVS1 CMV-MCS-Puro) Plasmid |
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| PVT15992 | Lifescience Market | 2 ug | EUR 325 |
 PinPoint-HR attP Placement Vector ? Empty MCS for cloning HR Arms |
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| PIN400A-1 | SBI | 10ug | EUR 1268 |
|
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 pMXs-EF1? Retroviral Vector |
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| RTV-063 | Cell Biolabs | 10 µg | EUR 624 |
|
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging |
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 pFC-CMV-MCS-pA-SV40-Neo PhiC31 Donor Vector |
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| FC501A-1 | SBI | 10ug | EUR 773 |
|
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 pLenti- EF1 |
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| PVT11083 | Lifescience Market | 2 ug | EUR 301 |
 pMK1200- EF1 |
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| PVT11119 | Lifescience Market | 2 ug | EUR 301 |
 BLIV 2.0 Reporter: CMV-Luciferase-EF1a-copGFP Lentivector Plasmid |
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| BLIV511PA-1 | SBI | 10 ug | EUR 803 |
|
|||
 (pORF)) MCS ORF Vector (Human) (pORF) |
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| ORF023399 | ABM | 1.0 ug DNA | Ask for price |
 pLenti-EF1a-EGFP-Puro-CMV-MCS-3Flag |
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| PVT17274 | Lifescience Market | 2 ug | EUR 474 |
) CMV control lentivirus (Hygro) |
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| CMV-Null-Hygro | GenTarget | 1 x107 IFU/ml x 200ul | EUR 349 |
|
Description: Negative control lentivirus contains a null spacer insert under CMV promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It has the hygromycin selection under RSV promoter. |
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) CMV control lentivirus (Zeo) |
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| CMV-Null-Zeo | GenTarget | 1 x107 IFU/ml x 200ul | EUR 349 |
|
Description: Negative control lentivirus contains a null spacer insert under CMV promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It has the Zeocin selection under RSV promoter. |
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 pMC.CMV-MCS-SV40polyA |
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| MN501A-1 | SBI | 10 ug | EUR 735 |
|
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 pMC.EF1-MCS-SV40polyA |
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| MN502A-1 | SBI | 10 ug | EUR 735 |
|
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 pMyosin-MCS-PSmOrange |
|||
| PVT17668 | Lifescience Market | 2 ug | EUR 383 |
 pECMV-MCS-Myc |
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| PVT17857 | Lifescience Market | 2 ug | EUR 341 |
 pLEGFP-MCS-FLAG |
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| PVT18164 | Lifescience Market | 2 ug | EUR 341 |
 pAAV- MCS Plasmid |
|||
| PVT2102 | Lifescience Market | 2 ug | EUR 241 |
 pT7- His- MCS |
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| PVT10595 | Lifescience Market | 2 ug | EUR 266 |
 pEU- E01- MCS |
|||
| PVT11379 | Lifescience Market | 2 ug | EUR 370 |
 pAAVS1-P-MCS |
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| PVT12296 | Lifescience Market | 2 ug | EUR 703 |
 pMD-AOX-MCS |
|||
| PVT14289 | Lifescience Market | 2 ug | EUR 911 |
 pCDNA3.1-MCS-6 |
|||
| PVT15973 | Lifescience Market | 2 ug | EUR 325 |
 pEnCMV-MCS-3 |
|||
| PVT17410 | Lifescience Market | 2 ug | EUR 300 |
 One-Step gDNA Removal and cDNA Synthesis SuperMix |
|||
| 20-abx09885920ulSystems | Abbexa |
|
|
|
|||
 PB-CMV-miR-302/367-EF1-GFP iPSC Vector |
|||
| PB-MIR302 | SBI | 10 ug | EUR 1844 |
|
|||
) CMV Control lentiviral particles (Bsd) |
|||
| CMV-Null-Bsd | GenTarget | 1 x107 IFU/ml x 200ul | EUR 349 |
|
Description: Negative control lentivirus contains a null spacer insert under CMV promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It also has the blasticidin marker under RSV promoter. |
|||
) CMV Control lentiviral particles (Neo) |
|||
| CMV-Null-Neo | GenTarget | 1 x107 IFU/ml x 200ul | EUR 349 |
|
Description: Negative control lentivirus contains a null spacer insert under CMV promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It also has the Neomycin marker under RSV promoter. |
|||
) CMV Control lentiviral particles (Puro) |
|||
| CMV-Null-Puro | GenTarget | 1 x107 IFU/ml x 200ul | EUR 349 |
|
Description: Negative control lentivirus contains a null spacer insert under CMV promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It also has the Puromycin marker under RSV promoter. |
|||
 CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid |
|||
| CASLV105PA-1 | SBI | 10 ug | EUR 697 |
|
|||
 pMXs-EF1-Puro Retroviral Vector |
|||
| RTV-060 | Cell Biolabs | 10 µg | EUR 624 |
|
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging |
|||
 pMXs-EF1-GFP Retroviral Vector |
|||
| RTV-061 | Cell Biolabs | 10 µg | EUR 624 |
|
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging |
|||
 pMXs-EF1-Bsd Retroviral Vector |
|||
| RTV-062 | Cell Biolabs | 10 µg | EUR 624 |
|
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging |
|||
Similar results were obtained after injection of the cells through the round window membrane, directly into the scala tympani canal or in Rosenthal in the modiolus of the cochlea basal turn. Imaging of the auditory bulla shows that bioluminescent signal passing through the tympanic membrane and the cracks in the wall of the bulla bone. After opening the auditory bulla, bioluminescent signal was coming from a round window. This is the first study showing that bioluminescence imaging allows visualization of luciferase-expressing cells injected into the intact guinea pig cochlea.
