The Human IL-23 Decoy Receptor Inhibits T-Cells Producing IL-17 by Genetically Engineered Mesenchymal Stem Cells.

MRI Tracking of SPIO- and Fth1-Labeled Bone Marrow Mesenchymal Stromal Cell Transplantation for Treatment of Stroke.

The immunomodulatory and self-renewable features of human adipose mesenchymal stem cells (HAD-MSCs) mark their importance in regenerative medicine. Interleukin 23 (IL-23) as proinflammatory cytokines Hitting the regulatory T cells (Treg) and promote the response of T helper 17 (Th17) and helper 1 (Th1) cells T. This path begins inflammation and immunosuppression in several autoimmune diseases. The present study was to produce recombinant IL-23 decoy receptor (RIL- 23R) using own-MSCs as a good candidate for the former purpose vivo cell-based gene therapy reduces inflammation in autoimmune disease. have-MSCs isolated from lipoaspirate and then marked by differentiation.

RIL- 23R is designed and cloned into pCDH-813A- 1 lentiviral vectors. Transduction-MSCs have done in MOI (multiplicity of infection) = 50 with EFI pCDH- α- RIL- 23R- copGFP PGK. RIL- 23R expression and octamer-binding transcription factor 4 (OCT- 4) was determined by real-time polymerase chain reaction (real time-PCR). nature renews itself tested by OCT- 4. 23R designed RIL- bioactivity evaluated by IL-17 and IL-10 expression of splenocytes of mice. cell differentiation has confirmed the isolation right-MSCs from lipoaspirate.

Restriction enzyme digestion and sequencing verified the successful cloning RIL- 23R in CD813A-1 lentiviral vectors. Green fluorescent protein (GFP) positive transduction level is up to 90%, and real-time PCR showed expression levels of RIL-23R. October-4 has the same expression pattern nontransduced-MSCs have and have-MSCs transduced / RIL-23R which showed that lentiviral vectors does not affect the characteristics of own-MSCs. Downregulation of IL-17, and upregulation of IL-10 showed activity right-MSCs have engineered.

The results show that transduced own-MSCs / RIL- 23R, express IL-23 decoy receptors, may provide a useful approach to basic research on cell-based gene therapy for autoimmune disorders.

 The Human IL-23 Decoy Receptor Inhibits T-Cells Producing IL-17 by Genetically Engineered Mesenchymal Stem Cells.
The Human IL-23 Decoy Receptor Inhibits T-Cells Producing IL-17 by Genetically Engineered Mesenchymal Stem Cells.

Bioluminescent imaging exogenous Stem Cells in Intact Guinea Pig Cochlear.

Stem cell-based therapy can be used to replace damaged or missing neurons in the cochlear nerve of a patient suffering from sensorineural hearing loss severe-to-profound. In order to achieve functional recovery in future clinical trials, knowledge about the survival of transplanted cells and their differentiation into functional neurons is a prerequisite. It calls for non-invasive in vivo visualization of cell and long-term monitoring of life and their fate after transplantation cochlea. We have investigated if the molecular optical imaging allows visualization of cells within the cochlea exogenous intact cadaver heads guinea pigs.

Transduced (stem) cells stably co-expressing fluorescent (copGFP) and bioluminescent (Luc2) reporter molecules, which are injected into the internal auditory meatus or directly to the cochlea through the round window. After the injection of cells into the internal auditory meatus, glowing light signal observed in the cavum conchae of the ear, indicating that the light generated by Luc2 passing through the tympanic membrane and external auditory meatus.

pCDH-CMV-MCS-EF1-RFP cDNA Cloning and Expression Vector

CD512B-1 10 ug
EUR 635

pCDH-CMV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector

CD513B-1 10 ug
EUR 635

pCDH-CMV-MCS-EF1-Neo cDNA Cloning and Expression Vector

CD514B-1 10 ug
EUR 635

pCDH-CMV-MCS-EF1-Hygro cDNA Cloning and Expression Vector

CD515B-1 10 ug
EUR 635

pCDH-CMV-MCS-EF1-RFP+Puro cDNA Cloning and Expression Vector

CD516B-2 10 ug
EUR 635

pCDH-EF1-MCS-IRES-GFP cDNA Cloning and Expression Vector

CD530A-2 10 ug
EUR 653

pCDH-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector

CD531A-2 10 ug
EUR 653

pCDH-EF1-MCS-IRES-Puro cDNA Cloning and Expression Vector

CD532A-2 10 ug
EUR 653

pCDH-EF1-MCS-IRES-Neo cDNA Cloning and Expression Vector

CD533A-2 10 ug
EUR 653

pCDH-UbC-MCS-EF1-Hygro cDNA Cloning and Expression Vector

CD615B-1 10 ug
EUR 635

pCDH-MSCV-MCS-EF1-Puro cDNA Cloning and Expression Vector

CD710B-1 10 ug
EUR 635

pCDH-MSCV-MCS-EF1-GFP cDNA Cloning and Expression Vector

CD711B-1 10 ug
EUR 635

pCDH-CMV-MCS cDNA Cloning and Expression Vector

CD500B-1 10 ug
EUR 544

pCDH- CMV- MCS- EF1- copGFP Plasmid

PVT2334 2 ug
EUR 325

pCDH-MSCV-MCS-EF1-GFP+Puro cDNA Cloning and Expression Vector

CD713B-1 10 ug
EUR 635

pCDH- EF1- MCS- T2A- copGFP

PVT2336 2 ug
EUR 325

pCDH-MCS-T2A-copGFP-MSCV Cloning and Expression Vector

CD523A-1 10 ug
EUR 653

pCDH-EF1-MCS-T2A-Puro cDNA Cloning and Expression Vector 10 ug

CD527A-1 10 ug
EUR 653

pCDH- CMV- MCS- EF1- CopGFP- T2A- Puro

PVT2333 2 ug
EUR 325

pCDF1-MCS2-EF1-copGFP cDNA Cloning and Expression Vector

CD111B-1 10 ug
EUR 635

pCDH-EF1-MCS-(PGK-Puro) Cloning and Expression Vector

CD810A-1 10 ug
EUR 642

pCDH-EF1-MCS-(PGK-GFP) Cloning and Expression Vector

CD811A-1 10 ug
EUR 642

PB-CMV-MCS-EF1-Puro cDNA cloning and expression vector

PB510B-1 10 ug
EUR 702

PB-CMV-MCS-EF1-GFP cDNA cloning and expression vector

PB511B-1 10 ug
EUR 702

PB-CMV-MCS-EF1-RFP cDNA cloning and expression vector

PB512B-1 10 ug
EUR 702

PB-CMV-MCS-EF1-GreenPuro cDNA cloning and expression vector

PB513B-1 10 ug
EUR 702

PB-CMV-MCS-EF1-RedPuro cDNA Cloning and Expression Vector

PB514B-2 10 ug
EUR 702

pCDH-EF1-MCS-BGH-PGK-GFP-T2A-Puro cDNA Cloning and Expression Vector

CD550A-1 10 ug
EUR 625

pCDH-CMV-MCS-EF1-copGFP cDNA Clon/Exp Vector [pre-packaged virus]

CD511VB-1 >1 x 10^6 IFUs
EUR 786

pCDH-CMV-MCS-EF1-copGFP+Puro cDNA Clon/Exp Vector [pre-packaged]

CD513VB-1 >1 x 10^6 IFUs
EUR 786

pCDH-CMV-MCS-EF1-copGFP-T2A-Puro plasmid

PVT17271 2 ug
EUR 474

pCDH-MCS-T2A-Puro-MSCV cDNA Cloning and Expression Vector

CD522A-1 10 ug
EUR 653

pCDH-UbC-MCS-IRES-GFP cDNA Cloning and Expression Vector

CD630A-1 10 ug
EUR 653

pCDH-MSCV-MCS-IRES-GFP cDNA Cloning and Expression Vector

CD731B-1 10 ug
EUR 653

pCDH- MSCV- MCS- EF1- CopGFP- T2A- Puro

PVT11079 2 ug
EUR 370

PB-EF1-MCS-IRES-GFP cDNA cloning and expression vector

PB530A-2 10 ug
EUR 702

PB-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector

PB531A-2 10 ug
EUR 702

PB-EF1-MCS-IRES-Neo cDNA cloning and expression vector

PB533A-2 10 ug
EUR 702

PB-MSCV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector

PB713B-1 10 ug
EUR 702

pCDH-CMV-MCS2 cDNA Cloning and Expression Vector

CD501A-1 10 ug
EUR 544

pCDH-EF1-MCS-(PGK-GFP-T2A-Puro) Cloning and Expression Vector

CD813A-1 10 ug
EUR 642

pCDH-EF1-MCS-T2A-RFP (PGK-Puro) Cloning and Expression Vector

CD822A-1 10 ug
EUR 642

pCDH-EF1-MCS-T2A-GFP (PGK-Puro) Cloning and Expression Vector

CD823A-1 10 ug
EUR 642

pCDH- CMV- MCS- EF1- puro

PVT11075 2 ug
EUR 370

pCDH- CMV- MCS- EF1- Hygro

PVT11076 2 ug
EUR 370

pAAVK-EF1? -MCS AAVanced Cloning and Expression Vector

AAV502A-1 10 ug
EUR 677

pCDH-CMV-MCS-EF1-copGFP cDNA Clon/Exp Vector [pre-packaged Ultra-High Titer virus]

CD511VB-UHT >1x10^9 IFUs
EUR 1041

pCDH-CMV-GSTA1-EF1-copGFP Plasmid

PVT16093 2 ug
EUR 325

pCDH- CMV- mCherry- EF1- CopGFP- Puro

PVT11078 2 ug
EUR 370

Scramble control hairpin in pCDH-CMV-MCS-EF1-copGFP (CD511B-1)

PMIRH000PA-1 Bacterial Streak
EUR 656

pCDH-EF1-Luc-T2A-copGFP

PVT17649 2 ug
EUR 424

pCDH-CMV-MCS-EF1-EGFP-IRFP

PVT17586 2 ug
EUR 424

pCDH-CMV-MCS-EF1-aRFP cDNA Clon/Exp Vector [pre-packaged]

CD512VA-1 >1 x 10^6 IFUs
EUR 786

pCDH-CMV-Akaluc-EF1-CopGFP-T2A-Puro

PVT14490 2 ug
EUR 599

pCDH-CMV-MCS-EF1-Puro cDNA Vector cDNA Clon/Exp Vector [pre-packaged virus]

CD510VB-1 >1 x 10^6 IFUs
EUR 786

pCDH-CMV-exosome booster-EF1-copGFP-Puro vector

PVT12109 2 ug
EUR 958

PB-Cuo-MCS-IRES-GFP-EF1-CymR-Puro Inducible cDNA Cloning and Expression Vector

PBQM812A-1 10 ug
EUR 1096

pCDH-EF1-MCS*-T2A-GFP

CD526A-1 10 ug
EUR 653

pAAVK-EF1? -MCS-T2A-EGFP AAVanced Cloning and Expression Vector

AAV526A-1 10 ug
EUR 677

pAAVK-EF1? -MCS-T2A-Puro AAVanced Cloning and Expression Vector

AAV527A-1 10 ug
EUR 677

pAAVK-EF1? ?MCS-T2A-mRFP AAVanced Cloning and Expression Vector

AAV528A-1 10 ug
EUR 677

PB-EF1-GreenPuro-H1-MCS shRNA cloning and expression vector

PBSI506A-1 10 ug
EUR 702

pCDH-CMV-MCS-EF1-RFP+Puro cDNA Clon/Exp Vector [pre-packaged]

CD516VB-2 >1 x 10^6 IFUs
EUR 772

pCDH-CMV-MCS-EF1-Puro (CD510B) plasmid

PVT17272 2 ug
EUR 474

pCDH- CMV- MCS- EF1- turboRFP- T2A- Puro

PVT11077 2 ug
EUR 370

pCDH-CMV-PTX3-EF1-CopGFP-T2A-Puro Plasmid

PVT17116 2 ug
EUR 325

pCDH-CMV-efemp1-m-EF1-CopGFP-T2A-Puro

PVT17438 2 ug
EUR 300

pCDH-CMV-TXNL1-HA-EF1-CopGFP-T2A-Puro

PVT17693 2 ug
EUR 258

pCDH-CMV-CCDC68-HA-EF1-CopGFP-T2A-Puro

PVT17760 2 ug
EUR 258

pCDH-CMV-ccl3-m-EF1-CopGFP-T2A-Puro

PVT19054 2 ug
EUR 300

pCDF1-MCS2-EF1-Puro cDNA Cloning and Expression Lentivector

CD110B-1 10 ug
EUR 635

pCDH- ECMV- MCS- 3*Flag- EF1

PVT11080 2 ug
EUR 370

pCDH- EF1- MCS- T2A- Puro Plasmid

PVT2335 2 ug
EUR 325

pCDH-CMV-MCS-EF1-Puro-FLAG-HIF2 alpha

PVT12166 2 ug
EUR 370

XStamp cloning and expression lentivector MSCV-Leader-MCS-C1C2-EF1-Puro

XSTP710PA-1 10 ug
EUR 797

pSIH1-H1-copGFP shRNA Cloning and Expression Vector

SI501A-1 10 ug
EUR 567

pCDH-EF1-MCS-T2A-Puro (CD520A) plasmid

PVT17273 2 ug
EUR 474

pCDH-Cuo-MCS-EF1-CymR-T2A-Puro

QM800A-1 10 ug
EUR 1310

pCDF1-MCS1 cDNA Cloning and Expression Vector

CD100A-1 10 ug
EUR 544

PB-CMV-GreenPuro-H1-MCS shRNA cloning and expression vector

PBSI505A-1 10 ug
EUR 702

pCDH-CuO-MCS-EF1-GFP dual promoter lentivector

QM511B-1 10 ug
EUR 679

pCDH-CuO-MCS-EF1-RFP dual promoter lentivector

QM512B-1 10 ug
EUR 679

pAAVK-EF1?-MCS1-CMV-MCS2 AAVanced Cloning and Expression Vector

AAV503A-1 10 ug
EUR 677

pAAVK-EF1?-MCS1-CMV-EGFP AAVanced Cloning and Expression Vector

AAV536A-1 10 ug
EUR 677

pAAVK-EF1?-MCS1-CMV-Puro AAVanced Cloning and Expression Vector

AAV537A-1 10 ug
EUR 677

pAAVK-EF1?-MCS1-CMV-mRFP AAVanced Cloning and Expression Vector

AAV538A-1 10 ug
EUR 677

pCDH-CuO-MCS empty cloning lentivector

QM500A-1 10 ug
EUR 597

pCDH-CuO-MCS-EF1-GFP+Puro dual promoter and dual marker lentivector

QM513B-1 10 ug
EUR 679

pCDH-CuO-MCS-EF1-RFP+Puro dual promoter and dual marker lentivector

QM516B-2 10 ug
EUR 679

XPack CMV-XP-MCS-EF1-Puro Cloning Lentivector

XPAK510PA-1 10 ug
EUR 797

pCDH-CMV-MCS-EF1a-GFP+BSD

PVT17282 2 ug
EUR 391

pCDH-CMV-exosome booster-EF1-Hygro

PVT12359 2 ug
EUR 2991

pCDH-CMV-Bach2-m-EF1-Puro

PVT13474 2 ug
EUR 703

pCDH-CMV-FGL1-EF1-Puro Plasmid

PVT16069 2 ug
EUR 325

pCDH-CMV-LAG3-EF1-Puro Plasmid

PVT16070 2 ug
EUR 325

pCDH-CMV-PDCD1-EF1-Hygro Plasmid

PVT16072 2 ug
EUR 325

Similar results were obtained after injection of the cells through the round window membrane, directly into the scala tympani canal or in Rosenthal in the modiolus of the cochlea basal turn. Imaging of the auditory bulla shows that bioluminescent signal passing through the tympanic membrane and the cracks in the wall of the bulla bone. After opening the auditory bulla, bioluminescent signal was coming from a round window. This is the first study showing that bioluminescence imaging allows visualization of luciferase-expressing cells injected into the intact guinea pig cochlea.

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