MRI Tracking of SPIO- and Fth1-Labeled Bone Marrow Mesenchymal Stromal Cell Transplantation for Treatment of Stroke.

We aim to identify a suitable method for long-term monitoring of the migration and proliferation of mesenchymal stromal cells in a rat model of stroke using transgene expression of ferritin by magnetic resonance imaging (MRI). bone marrow mesenchymal stromal cells (BMSCs) were transduced with lentivirus containing plasmid shuttle (pCDH-CMV-MCS-EF1-copGFP) brings ferritin heavy chain 1 (Fth1) gene. Ferritin expression in stromal cells was evaluated by western blotting and immunofluorescent staining.

Absorption of iron from Fth1-BMSCs measured by Prussian blue staining. Following the introduction of surgical occlusion of the middle cerebral artery, Fth1-BMSCs and superparamagnetic iron oxide- (SPIO-) labeled BMSCs were injected via the internal jugular vein. Imaging and the intensity of the signal being monitored by diffusion-weighted imaging (DWI), T2-weighted imaging (T2WI), and susceptibility-weighted imaging (SWI) in vitro and in vivo. Pathology conducted for comparison. We observed that the MRI signal intensity of SPIO-BMSCs gradually reduced over time.

Fth1-BMSCs showed the same signal intensity between 10 and 60 days. SWI shows hypointense lesions in SPIO-BMSC (traceable to 30 d) and Fth1-BMSC group. T2WI not sensitive enough to track Fth1-BMSCs. After transplantation, the cells stained with Prussian blue was observed around the central area of ​​infarction and infarction in both transplantation models. Fth1-BMSCs transplanted to treat focal cerebral infarction safe, reliable, and can be tracked by MRI. Fth1 more stable and suitable labeling of SPIO labeling for long-term tracking. SWI is more sensitive than T2W1 and suitable as MRI-tracking optimal sequence.

 MRI Tracking of SPIO- and Fth1-Labeled Bone Marrow Mesenchymal Stromal Cell Transplantation for Treatment of Stroke.
MRI Tracking of SPIO- and Fth1-Labeled Bone Marrow Mesenchymal Stromal Cell Transplantation for Treatment of Stroke.

Design and analysis of integrated reporter’s stable transgene expression is induced in human T cells and NK-cell line CAR.

the cytotoxic activity of T and NK-cells can be efficiently Retargeted against cancer cells using chimeric antigen receptor (CAR) and rTCRs. In the context of solid cancers, the use of armored cells CAR T and NK secrete a molecule anti-cancer extras such as cytokines, chemokines, antibodies, bite, cytokine receptor inverse, and inhibitors of the checkpoint, appears particularly promising, as this can help to overcome the immunosuppressive tumor micro , attracting an audience of immune cells, and increasing the CAR T / NK-cell persistence.

Placing such molecule expression under the control of the downstream transcription activation offers T / NK-cell-mediated CAR advantages of targeted delivery, high local concentrations and reduced toxicity. Some canonical DNA sequences are known to function as a promoter-induced activation in human T and B cells have been described to date and usually include multimers of NFkB and NFAT binding sites. However, relatively little is known about the sequence of DNA that can serve as a switch in a context-driven activation of NK cells.

pCDH-CMV-MCS2 cDNA Cloning and Expression Vector

CD501A-1 10 ug
EUR 483

pCDH-EF1-MCS cDNA Cloning and Expression Vector

CD502A-1 10 ug
EUR 483

pCDH-CMV-MCS-t2A-copGFP cDNA Cloning and Expression Vector

CD524A-1 10 ug
EUR 590

pCDH-CMV-MCS-EF1-RFP cDNA Cloning and Expression Vector

CD512B-1 10 ug
EUR 572

pCDH-CMV-MCS-EF1-Neo cDNA Cloning and Expression Vector

CD514B-1 10 ug
EUR 572

pCDH-CMV-MCS-EF1-Puro cDNA Cloning and Expression Vector

CD510B-1 10 ug
EUR 572

pCDH-EF1-MCS-IRES-GFP cDNA Cloning and Expression Vector

CD530A-2 10 ug
EUR 590

pCDH-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector

CD531A-2 10 ug
EUR 590

pCDH-EF1-MCS-IRES-Neo cDNA Cloning and Expression Vector

CD533A-2 10 ug
EUR 590

pCDH-MSCV-MCS-EF1-GFP cDNA Cloning and Expression Vector

CD711B-1 10 ug
EUR 572

pCDH-CMV-MCS-EF1-Hygro cDNA Cloning and Expression Vector

CD515B-1 10 ug
EUR 572

pCDH-UbC-MCS-EF1-Hygro cDNA Cloning and Expression Vector

CD615B-1 10 ug
EUR 572

pCDH-EF1-MCS-IRES-Puro cDNA Cloning and Expression Vector

CD532A-2 10 ug
EUR 590

pCDH-MSCV-MCS-EF1-Puro cDNA Cloning and Expression Vector

CD710B-1 10 ug
EUR 572

pCDH-CMV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector

CD513B-1 10 ug
EUR 572

pCDH-CMV-MCS-EF1-RFP+Puro cDNA Cloning and Expression Vector

CD516B-2 10 ug
EUR 572

PB-CMV-MCS-EF1-GFP cDNA cloning and expression vector

PB511B-1 10 ug
EUR 638

PB-CMV-MCS-EF1-RFP cDNA cloning and expression vector

PB512B-1 10 ug
EUR 638

pCDH-MSCV-MCS-EF1-GFP+Puro cDNA Cloning and Expression Vector

CD713B-1 10 ug
EUR 572

PB-CMV-MCS-EF1-Puro cDNA cloning and expression vector

PB510B-1 10 ug
EUR 638

PB-EF1-MCS-IRES-GFP cDNA cloning and expression vector

PB530A-2 10 ug
EUR 638

PB-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector

PB531A-2 10 ug
EUR 638

PB-EF1-MCS-IRES-Neo cDNA cloning and expression vector

PB533A-2 10 ug
EUR 638

PB-CMV-MCS-EF1-RedPuro cDNA Cloning and Expression Vector

PB514B-2 10 ug
EUR 638

pCDH-EF1-MCS-T2A-Puro cDNA Cloning and Expression Vector 10 µg

CD527A-1 10 ug
EUR 590

pCDH-CMV-MCS cDNA Cloning and Expression Vector

CD500B-1 10 ug
EUR 483

PB-CMV-MCS-EF1-GreenPuro cDNA cloning and expression vector

PB513B-1 10 ug
EUR 638

PB-MSCV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector

PB713B-1 10 ug
EUR 638

pCDH-EF1-MCS-BGH-PGK-GFP-T2A-Puro cDNA Cloning and Expression Vector

CD550A-1 10 ug
EUR 563

pCDH-UbC-MCS-IRES-GFP cDNA Cloning and Expression Vector

CD630A-1 10 ug
EUR 590

pCDH-MCS-T2A-Puro-MSCV cDNA Cloning and Expression Vector

CD522A-1 10 ug
EUR 590

pCDH-MSCV-MCS-IRES-GFP cDNA Cloning and Expression Vector

CD731B-1 10 ug
EUR 590

PB-Cuo-MCS-IRES-GFP-EF1-CymR-Puro Inducible cDNA Cloning and Expression Vector

PBQM812A-1 10 ug
EUR 1023

pCDH-MCS-T2A-copGFP-MSCV Cloning and Expression Vector

CD523A-1 10 ug
EUR 590

pSIH1-H1-copGFP shRNA Cloning and Expression Vector

SI501A-1 10 ug
EUR 507

pCDH-EF1-MCS-(PGK-GFP) Cloning and Expression Vector

CD811A-1 10 ug
EUR 579

pCDH-EF1-MCS-(PGK-Puro) Cloning and Expression Vector

CD810A-1 10 ug
EUR 579

PB-CuO-CMV-MCS-EF1α-CymR-Puro Inducible cDNA Cloning and Expression Vector

PBQM800A-1 10 µg
EUR 921

pAAVK-EF1α -MCS AAVanced Cloning and Expression Vector

AAV502A-1 10 ug
EUR 614

pAAVK-EF1α-MCS1-CMV-MCS2 AAVanced Cloning and Expression Vector

AAV503A-1 10 ug
EUR 614

pCDH-EF1-MCS-T2A-RFP (PGK-Puro) Cloning and Expression Vector

CD822A-1 10 ug
EUR 579

pCDH-EF1-MCS-T2A-GFP (PGK-Puro) Cloning and Expression Vector

CD823A-1 10 ug
EUR 579

pCDH-EF1-MCS-(PGK-GFP-T2A-Puro) Cloning and Expression Vector

CD813A-1 10 ug
EUR 579

PB-EF1-GreenPuro-H1-MCS shRNA cloning and expression vector

PBSI506A-1 10 ug
EUR 638

pAAVK-EF1α -MCS-T2A-EGFP AAVanced Cloning and Expression Vector

AAV526A-1 10 ug
EUR 614

pAAVK-EF1α -MCS-T2A-Puro AAVanced Cloning and Expression Vector

AAV527A-1 10 ug
EUR 614

pAAVK-EF1α –MCS-T2A-mRFP AAVanced Cloning and Expression Vector

AAV528A-1 10 ug
EUR 614

pSIH1-H1-Puro shRNA Cloning and Expression Vector

SI500A-1 10 ug
EUR 507

pSIH1-H1-H2Kk shRNA Cloning and Expression Vector

SI502A-1 10 ug
EUR 507

Cumate-Inducible (CuO) EEV cloning and expression vector

EEV610A-1 10 ug
EUR 614

pCDF1- MCS2- EF1- Puro Plasmid

PVT2338 2 ug
EUR 319.2

Constitutive (CAGs promoter) EEV cloning and expression vector

EEV600A-1 10 ug
EUR 614

PB-Cuo-shMCS-IRES-GFP-EF1-CymR-Puro Inducible shRNA Cloning and Expression Vector

PBQMSH812A-1 10 ug
EUR 1023

PB-CMV-GreenPuro-H1-MCS shRNA cloning and expression vector

PBSI505A-1 10 ug
EUR 638

pAAVK-EF1α-MCS1-CMV-EGFP AAVanced Cloning and Expression Vector

AAV536A-1 10 ug
EUR 614

pAAVK-EF1α-MCS1-CMV-Puro AAVanced Cloning and Expression Vector

AAV537A-1 10 ug
EUR 614

pAAVK-EF1α-MCS1-CMV-mRFP AAVanced Cloning and Expression Vector

AAV538A-1 10 ug
EUR 614

XStamp cloning and expression lentivector MSCV-Leader-MCS-C1C2-EF1-Puro

XSTP710PA-1 10 ug
EUR 730

pLenti-SFFV-MCS-EF1-copGFP Lentiviral Expression Vector

LV460 10 µg
EUR 416.5

pCDH- EF1- MCS- T2A- copGFP

PVT2336 2 ug
EUR 390

baculoCOMPLETE protein expression kit

400100 5 reactions
EUR 874.2

pCDH- CMV- MCS- EF1- copGFP Plasmid

PVT2334 2 ug
EUR 390

pCDH- CMV- MCS- EF1- CopGFP- T2A- Puro

PVT2333 2 ug
EUR 390

pCDH- MSCV- MCS- EF1- CopGFP- T2A- Puro

PVT11079 2 ug
EUR 444

pLenti-EF1-MCS Lentiviral Expression Vector

LV200 10 µg
EUR 416.5

baculoCOMPLETE protein expression kit + baculoQUANT all-in-one

400101 5 + 100 reactions
EUR 1419.8

pLenti-CAG-MCS- EF1-coGFP Lentiviral Expression Vector

LV360 10 µg
EUR 416.5

pLenti-CAG-MCS-PGK-copGFP Lentiviral Expression Vector

LV310 10 µg
EUR 416.5

pLenti-MCS-EF1-GFP Lentiviral Expression Vector

LV010 10 µg
EUR 416.5

pLenti-EF1-MCS-PGK-Neo Lentiviral Expression Vector

LV202 10 µg
EUR 416.5

pLenti-EF1-MCS-PGK-GFP Lentiviral Expression Vector

LV210 10 µg
EUR 416.5

pLenti-EF1-MCS-PGK-RFP Lentiviral Expression Vector

LV220 10 µg
EUR 416.5

pLenti-EF1-MCS-PGK-PURO Lentiviral Expression Vector

LV201 10 µg
EUR 416.5

pLenti-CAG-MCS-EF1-PURO Lentiviral Expression Vector

LV351 10 µg
EUR 416.5

pLenti-EF1-MCS-PGK-Hygro Lentiviral Expression Vector

LV203 10 µg
EUR 416.5

pLenti-SFFV-MCS-EF1-Puro Lentiviral Expression Vector

LV451 10 µg
EUR 416.5

pLenti-CAG-MCS-EF1-TdTomato Lentiviral Expression Vector

LV370 10 µg
EUR 416.5

pLenti-SFFV-MCS-EF1-TdTomato Lentiviral Expression Vector

LV470 10 µg
EUR 416.5

pRedPuro shRNA expression lentivector (EF1)

SI516A-1 10 ug
EUR 594

pGreenPuro shRNA expression lentivector (EF1)

SI506A-1 10 ug
EUR 594

pLenti-MCS-EF1-GFP-T2A-puro Lentiviral Expression Vector

LV011 10 µg
EUR 416.5

pLenti-MCS-EF1-RFP-T2A-puro Lentiviral Expression Vector

LV021 10 µg
EUR 416.5

CNGA2 Expression vector

60642 20 µg
EUR 900
Description: The CNGA2 expression vector is designed to express human cyclic nucleotide-gated cation channel 2 (CNGA2) in mammalian cells.

pYLEX1 - Expression Vector

FYY203-5MG 5mg Ask for price

Bicistronic Expression: pCHAC-MCS1-IRES-MCS2

ABP-PVL-BICISIRES 5 ug Ask for price

pLenti-EF1-MCS-PGK-GFP-T2A-PURO Lentiviral Expression Vector

LV211 10 µg
EUR 416.5

pLenti-EF1-MCS-PGK-GFP-T2A-Neo Lentiviral Expression Vector

LV212 10 µg
EUR 416.5

pLenti-EF1-MCS-PGK-RFP-T2A-PURO Lentiviral Expression Vector

LV221 10 µg
EUR 416.5

pCDH- CMV- mCherry- EF1- CopGFP- Puro

PVT11078 2 ug
EUR 444

Rac1 Expression Vector Set

STA-454 1 kit
EUR 1765.2
Description: Rac1 Expression Vector Set contains 3 vectors: Rac wild type, T17N dominant negative mutant, and G12V constitutively active mutant.

RhoA Expression Vector Set

STA-456 1 kit
EUR 1765.2
Description: RhoA Expression Vector Set contains 3 vectors: RhoA wild type, T19N dominant negative mutant, and G14V constitutively active mutant.

pLenti-EF1-MCS-PGK-GFP-T2A-Hygro Lentiviral Expression Vector

LV213 10 µg
EUR 416.5

pLenti-CAG-MCS-EF1-coGFP-T2A-PURO Lentiviral Expression Vector

LV361 10 µg
EUR 416.5

Cdc42 Expression Vector Set

STA-455 1 kit
EUR 1765.2
Description: Cdc42 Expression Vector Set contains 3 vectors: Cdc42 wild type, T17N dominant negative mutant, and Q61L constitutively active mutant.

pAAV-MCS Expression Vector

VPK-410 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pLenti-SFFV-MCS-EF1-coGFP-T2A-Puro Lentiviral Expression Vector

LV461 10 µg
EUR 416.5

pLenti-CAG-MCS-EF1-TdTomato-T2A-Puro Lentiviral Expression Vector

LV371 10 µg
EUR 416.5

pLenti-SFFV-MCS-EF1-TdTomato-T2A-Puro Lentiviral Expression Vector

LV471 10 µg
EUR 416.5

pscAAV-MCS Expression Vector

VPK-430 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

H-Ras Expression Vector Set

STA-457 1 kit
EUR 1765.2
Description: H-Ras Expression Vector Set contains 3 vectors: H-Ras wild type, T19N dominant negative mutant, and G12V constitutively active mutant.

pUC57-sgRNA expression vector

PVT18003 2 ug
EUR 309.6

ANPRA/Aequorin Expression vector

60641 20 µg
EUR 900
Description: The ANPRA/Aequorin expression vector is designed to co-express human atrial natriuretic peptide receptor A (ANPRA, also called natriuretic peptide receptor A/guanylate cyclase A) and jellyfish (Aequorea victoria) Aequorin in mammalian cells.

GFP-Rac1 Expression Vector Set

STA-450 1 kit
EUR 1939.2
Description: GFP-Rac1 Expression Vector Set contains 3 vectors: Rac wild type, T17N dominant negative mutant, and Q61L constitutively active mutant. Each vector also contains a GFP reporter sequence.

GFP-RhoA Expression Vector Set

STA-452 1 kit
EUR 1939.2
Description: GFP-RhoA Expression Vector Set contains 3 vectors: RhoA wild type, T19N dominant negative mutant, and Q63L constitutively active mutant. Each vector also contains a GFP reporter sequence.

GFP-Cdc42 Expression Vector Set

STA-451 1 kit
EUR 1939.2
Description: GFP-Cdc42 Expression Vector Set contains 3 vectors: Cdc42 wild type, T17N dominant negative mutant, and Q61L constitutively active mutant. Each vector also contains a GFP reporter sequence.

Active Rac1 Expression Vector Set

STA-458 1 kit
EUR 1765.2
Description: Active Rac1 Expression Vector Set contains 3 vectors expressing different constitutively active mutants of Rac1: Q61L, Q61L/F37A, and Q61L/Y40C.

pCMV-GFP-LC3 Expression Vector

CBA-401 100 µL
EUR 1243.2
Description: Expression vector contains a fusion of GFP and LC3. A separate GFP control vector is also included.

Tet-on circRNA Expression Vector

PVT14643 2 ug
EUR 843.6

pAAV-IRES-Neo Expression Vector

VPK-416 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-IRES-GFP Expression Vector

VPK-418 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-IRES-Bsd Expression Vector

VPK-419 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-IRES-Puro Expression Vector

VPK-415 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-IRES-Hygro Expression Vector

VPK-417 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

Active H-Ras Expression Vector Set

STA-459 1 kit
EUR 1765.2
Description: Active H-Ras Expression Vector Set contains 3 vectors expressing different constitutively active mutants of H-Ras: V12, V12S35, and V12C40.

AAVS1 Safe Harbor cDNA/miRNA Donor Vector [pAAVS1D-PGK.MCS-EF1a.copGFPpuro]

GE602A-1 10 ug
EUR 1190

pAAV-MCS Promoterless Expression Vector

VPK-411 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pSMPUW-Neo Lentiviral Expression Vector

VPK-213 10 µg
EUR 748.8
Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer.

pLenti-MCS Lentiviral Expression Vector

LV000 10 µg
EUR 416.5

pSMPUW-Puro Lentiviral Expression Vector

VPK-212 10 µg
EUR 748.8
Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer.

pSMPUW-Hygro Lentiviral Expression Vector

VPK-214 10 µg
EUR 748.8
Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer.

Baculovirus expression vector pAc-g-CH3

30R-AB001 5 ug
EUR 739.2
Description: Baculovirus cassette vector pAc-l-CH3 for the expression of human, humanized or chimeric IgG(lambda) in insect cells and secretion of assembled antibodies into the supernatant.

Exoenzyme C3 (Rho Inhibitor) Expression Vector

STA-460 1 kit
EUR 860.4
Description: This mammalian expression vector codes for Exoenzyme C3, a Rho inhibitor.

pSMPUW-GFP-LC3 Lentiviral Expression Vector

LTV-801 10 µg
EUR 1444.8
Description: Expression vector contains a fusion of GFP and LC3. A separate GFP control vector is also included.

pLenti-CMV-MCS Lentiviral Expression Vector

LV100 10 µg
EUR 416.5

pLenti-CAG-MCS Lentiviral Expression Vector

LV300 10 µg
EUR 416.5

pSMPUW-IRES-Neo Lentiviral Expression Vector

VPK-216 10 µg
EUR 748.8
Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer.

pLenti-SFFV-MCS Lentiviral Expression Vector

LV400 10 µg
EUR 416.5

pSMPUW-IRES-Puro Lentiviral Expression Vector

VPK-215 10 µg
EUR 748.8
Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer.

pSMPUW-IRES-Hygro Lentiviral Expression Vector

VPK-217 10 µg
EUR 748.8
Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer.

Mouse IL-23 Enhanced Episomal Expression Vector

EEV651A-1 100 ug
EUR 812

XPack CMV-XP-GFP-EF1-Puro Expression Lentivirus

XPAK530VA-1 >1 x 10^6 IFUs
EUR 759

XPack CMV-XP-GFP-EF1-Puro Expression Lentivector

XPAK530PA-1 10 ug
EUR 759

XPack CMV-XP-RFP-EF1-Puro Expression Lentivector

XPAK531PA-1 10 ug
EUR 759

XPack MSCV-XP-GFP-EF1-Puro Expression Lentivirus

XPAK730VA-1 >1 x 10^6 IFUs
EUR 759

pMXs-miRNA-GFP/Puro Retroviral Expression Vector

RTV-017 10 ?g
EUR 748.8
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

XPack MSCV-XP-GFP-EF1-Puro Expression Lentivector

XPAK730PA-1 10 ug
EUR 759

pCDH-CMV-MCS-EF1-copGFP+Puro cDNA Clon/Exp Vector [pre-packaged]

CD513VB-1 >1 x 10^6 IFUs
EUR 720

pCDH-CMV-MCS-EF1-copGFP cDNA Clon/Exp Vector [pre-packaged virus]

CD511VB-1 >1 x 10^6 IFUs
EUR 720

pLenti-MCS-PGK-PURO Lentiviral Expression Vector

LV001 10 µg
EUR 416.5

pSMPUW-IRES-Blasticidin Lentiviral Expression Vector

VPK-219 10 µg
EUR 748.8
Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer.

pSMPUW Universal Lentiviral Expression Vector (Promoterless)

VPK-211 10 µg
EUR 748.8
Description: Clone your gene of interest and a gene-specific promoter into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293T or 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer.

XPack CMV-XP-Luciferase-EF1-Puro Expression Lentivirus

XPAK532VA-1 >1 x 10^6 IFUs
EUR 759

XPack CMV-XP-Luciferase-EF1-Puro Expression Lentivector

XPAK532PA-1 10 ug
EUR 759

XPack MSCV-XP-Luciferase-EF1-Puro Expression Lentivector

XPAK732PA-1 10 ug
EUR 759

XPack MSCV-XP-Luciferase-EF1-Puro Expression Lentivirus

XPAK732VA-1 >1 x 10^6 IFUs
EUR 759

pLenti-CAG-MCS-PGK-NEO Lentiviral Expression Vector

LV302 10 µg
EUR 416.5

Excision only piggyBac Transposase expression vector, 50 rxns

PB220PA-1 10 ug
EUR 471

pLenti-CMV-MCS-EF1α-GFP Lentiviral Expression Vector

LV110 10 µg
EUR 416.5

pLenti-CMV-MCS-EF1α-RFP Lentiviral Expression Vector

LV120 10 µg
EUR 416.5

pLenti-CAG-MCS-PGK-Puro Lentiviral Expression Vector

LV301 10 µg
EUR 416.5

pLenti-CAG-MCS-PGK-HygR Lentiviral Expression Vector

LV303 10 µg
EUR 416.5

pLenti-SFFV-MCS-PGK-NEO Lentiviral Expression Vector

LV402 10 µg
EUR 416.5

pLenti-SFFV-MCS-PGK-GFP Lentiviral Expression Vector

LV410 10 µg
EUR 416.5

pLenti-CMV-MCS-EF1α-PURO Lentiviral Expression Vector

LV101 10 µg
EUR 416.5

pLenti-SFFV-MCS-PGK-Puro Lentiviral Expression Vector

LV401 10 µg
EUR 416.5

pLenti-SFFV-MCS-PGK-Hygro Lentiviral Expression Vector

LV403 10 µg
EUR 416.5

pLenti-CAG-MCS-PGK-TdTomato Lentiviral Expression Vector

LV320 10 µg
EUR 416.5

Protein expression

CRO-4-Ind each Ask for price

pLenti-SFFV-MCS-PGK-TdTomato Lentiviral Expression Vector

LV420 10 µg
EUR 416.5

YLEX Expression Kit

FYY201-1KT 1 kit Ask for price

Super piggyBac Transposase expression vector (replaces PB200A-1), 50 rxns

PB210PA-1 10 ug
EUR 471

293 Expression MAX-1

EXP-711-11 1ml
EUR 214
Description: 293 Expression MAX-1 is expressed from - -.

pLenti-CMV-MCS-EF1α-GFP-T2A-PURO Lentiviral Expression Vector

LV111 10 µg
EUR 416.5

pLenti-CMV-MCS-EF1α-RFP-T2A-PURO Lentiviral Expression Vector

LV121 10 µg
EUR 416.5

pLenti-CAG-MCS-PGK-coGFP-T2A-Puro Lentiviral Expression Vector

LV311 10 µg
EUR 416.5

pLenti-CAG-MCS-PGK-coGFP-T2A-NEO Lentiviral Expression Vector

LV312 10 µg
EUR 416.5

pLenti-CAG-MCS-PGK-coGFP-T2A-HygR Lentiviral Expression Vector

LV313 10 µg
EUR 416.5

pLenti-SFFV-MCS-PGK-coGFP-T2A-NEO Lentiviral Expression Vector

LV412 10 µg
EUR 416.5

Blunt E1 Expression Kit

abx098098-10rxns 10 rxns
EUR 560.4

Blunt E2 Expression Kit

abx098873-10rxns 10 rxns
EUR 560.4

RFP expression Adenovirus

AVP001 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made RFP expression adenovirus, provided in DMEM medium.

CFP expression Adenovirus

AVP002 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made CFP expression adenovirus, provided in DMEM medium.

GFP expression Adenovirus

AVP011 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made GFP expression adenovirus, provided in DMEM medium.

YFP expression Adenovirus

AVP012 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made YFP expression adenovirus, provided in DMEM medium.

BFP expression Adenovirus

AVP017 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made BFP expression adenovirus, provided in DMEM medium.

pLenti-SFFV-MCS-PGK-coGFP-T2A-Puro Lentiviral Expression Vector

LV411 10 µg
EUR 416.5

pLenti-SFFV-MCS-PGK-coGFP-T2A-HygR Lentiviral Expression Vector

LV413 10 µg
EUR 416.5

pLenti-CAG-MCS-PGK-TdTomato-T2A-Puro Lentiviral Expression Vector

LV321 10 µg
EUR 416.5

pLenti-SFFV-MCS-PGK-TdTomato-T2A-Puro Lentiviral Expression Vector

LV421 10 µg
EUR 416.5

h ADA Expression Lentivirus

LVP923 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: ADA, containing a RFP-Blasticidin dual selection marker.

h IL6 Expression Lentivirus

LVP1144 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.

h CD4 Expression Lentivirus

LVP1152 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: CD4, containing a RFP-Blasticidin dual selection marker.

h IL4 Expression Lentivirus

LVP1161 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: IL4, containing a RFP-Blasticidin dual selection marker.

h IL7 Expression Lentivirus

LVP1162 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: IL7, containing a RFP-Blasticidin dual selection marker.

h KDR Expression Lentivirus

LVP1176 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: KDR, containing a RFP-Blasticidin dual selection marker.

h FAS Expression Lentivirus

LVP1181 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: FAS, containing a RFP-Blasticidin dual selection marker.

h OCT4 expression Adenovirus

AVP013 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made adenovirus express human stem cell factor, OCT4, provided in DMEM medium.

h SOX2 expression Adenovirus

AVP014 1x109 IFU/ml x 50ul
EUR 418.8
Description: pre-made adenovirus express human stem cell factor, SOX2, provided in DMEM medium.

h CDR1 Expression Lentivirus

LVP917 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: CDR1, containing a RFP-Blasticidin dual selection marker.

h CD47 Expression Lentivirus

LVP918 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: CD47, containing a RFP-Blasticidin dual selection marker.

We set out to compare the functionality of some activation-induced promoter in primary human T cells, as well as in the line of NK cells and NK-92 construction YT.Lentiviral engineered to express two fluorescent reporters: mCherry under 4xNFAT, 2xNFkB, 5xNFkB, 10xNFkB, 30xNFkB promoter, as well as two promoter variants of CD69, and copGFP under a strong constitutive promoter of the gene of human EF1a. Pseudotyped lentiviral particles obtained using the construction transduced into human primary T cells and NK-92 and certain cell lines expressing YT CAR to PSMA. Transgenic cells derived activated by CD3 / CD28 beads (T cell) or via CAR (CAR-NK cell line).

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